| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab121935-10μl | 10μl | In stock | $66.90 | |
| Ab121935-50μl | 50μl | In stock | $189.90 | |
| Ab121935-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab121935-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human Pirh2 Antibody; WB; Unconjugated
| Product Name | Recombinant Pirh2 Antibody - Primary antibody, specific to RCHY1, Rabbit IgG |
|---|---|
| Synonyms | Androgen receptor N terminal interacting protein antibody | Androgen receptor N-terminal-interacting protein antibody | ARNIP antibody | CH-rich-interacting match with PLAG1 antibody | CHIMP antibody | E3 ubiquitin-protein ligase Pirh2 antibody | hARNIP a |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.5 mg/mL |
| Host species | Rabbit |
| Specificity | RCHY1 |
| Immunogen | A synthetic peptide derived from human RCHY1 (AA 160-261). |
| Positive Control | WB: HEK293, HT-29, LNCaP and K562 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Pirh2 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Pirh2 Antibody (Ab121935) - Western Blot
All lanes: Recombinant Pirh2 Antibody (Ab121935) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 23.9 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0202934 | Certificate of Analysis | Mar 01, 2024 | Ab121935 |
| ZJ24F0202935 | Certificate of Analysis | Mar 01, 2024 | Ab121935 |
| 1. Logan, Ian R IR, Sapountzi, Vasileia V, Gaughan, Luke L, Neal, David E DE and Robson, Craig N CN.. (2004) Control of human PIRH2 protein stability: involvement of TIP60 and the proteosome.. The Journal of biological chemistry, (19): [PMID:14701804] |
| 2. Zhang, Liping L, Li, Jinjun J, Wang, Chunyan C, Ma, Yating Y and Huo, Keke K.. (2005) A new human gene hNTKL-BP1 interacts with hPirh2.. Biochemical and biophysical research communications, (29): [PMID:15781263] |
| 3. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 4. Logan, Ian R IR and 5 more authors.. (2006) Human PIRH2 enhances androgen receptor signaling through inhibition of histone deacetylase 1 and is overexpressed in prostate cancer.. Molecular and cellular biology, [PMID:16914734] |
| 5. Maruyama, Satoru S and 6 more authors.. (2008) Ubiquitylation of epsilon-COP by PIRH2 and regulation of the secretion of PSA.. Molecular and cellular biochemistry, [PMID:17721809] |
| 6. Zheng, Gang G, Ning, Jinying J and Yang, Yu-Chung YC.. (2007) PLAGL2 controls the stability of Pirh2, an E3 ubiquitin ligase for p53.. Biochemical and biophysical research communications, (14): [PMID:17950244] |
| 7. Hattori, Takayuki T and 7 more authors.. (2007) Pirh2 promotes ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor p27Kip1.. Cancer research, (15): [PMID:18006823] |
| 8. Zahedi, René P RP and 8 more authors.. (2008) Phosphoproteome of resting human platelets.. Journal of proteome research, [PMID:18088087] |
| 9. Sheng, Yi Y and 11 more authors.. (2008) Molecular basis of Pirh2-mediated p53 ubiquitylation.. Nature structural & molecular biology, [PMID:19043414] |
| 10. Corcoran, Chad A CA and 5 more authors.. (2009) Identification and characterization of two novel isoforms of Pirh2 ubiquitin ligase that negatively regulate p53 independent of RING finger domains.. The Journal of biological chemistry, (14): [PMID:19483087] |
| 11. Wu, Gang G and 5 more authors.. (2010) A novel hPirh2 splicing variant without ubiquitin protein ligase activity interacts with p53 and is down-regulated in hepatocellular carcinoma.. FEBS letters, (2): [PMID:20452352] |
| 12. Jung, Yong-Sam YS, Hakem, Anne A, Hakem, Razqallah R and Chen, Xinbin X.. (2011) Pirh2 E3 ubiquitin ligase monoubiquitinates DNA polymerase eta to suppress translesion DNA synthesis.. Molecular and cellular biology, [PMID:21791603] |
| 13. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
