Recombinant PODXL Antibody - Primary antibody, specific to PODXL, Rabbit IgG

    Application:
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab122517
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab122517-10μl
10μl
In stock
$66.90
Ab122517-50μl
50μl
In stock
$189.90
Ab122517-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab122517-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,809.90

Recombinant; Rabbit anti Human PODXL Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant PODXL Antibody - Primary antibody, specific to PODXL, Rabbit IgG
SynonymsGCTM-2 antigen antibody | Gp2 antibody | Gp200 antibody | MGC138240 antibody | PC antibody | PCLP antibody | PCLP-1 antibody | PCLP1 antibody | Pcx antibody | Podocalyxin antibody | Podocalyxin like antibody | Podocalyxin like protein antibody | Podocalyx
Specifications & PurityExactAb™, Validated, Recombinant, 0.6 mg/mL
Host speciesRabbit
SpecificityPODXL
ImmunogenA synthetic peptide derived from human PODXL (AA 300-479).
Positive ControlWB: HEK293, Raji, HeLa and HT-29 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human PODXL Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function
Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.6 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant PODXL Antibody (Ab122517) - Western Blot
All lanes: Recombinant PODXL Antibody (Ab122517) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution

Predicted band size: 59 kDa
Observed band size: 164,183 kDa
Exposure time: 76.5 s

Recombinant PODXL Antibody (Ab122517) - Western Blot
All lanes: Recombinant PODXL Antibody (Ab122517) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 59 kDa
Observed band size: 164, 183 kDa
Exposure time: 28.3 s

Associated Targets(Human)

PODXL Tbio Podocalyxin (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
UBQLN1 Tbio Ubiquilin-1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

Certificates

Certificate of Analysis(COA)

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Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0302993Certificate of AnalysisMar 04, 2024 Ab122517
ZJ24F0302992Certificate of AnalysisMar 04, 2024 Ab122517

Related Documents

References

1. Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al..  (2003)  Human chromosome 7: DNA sequence and biology..  Science,  300  (5620): (767-72).  [PMID:12690205] [10.1021/op500134e]
2. Kershaw, D B DB and 6 more authors..  (1997)  Molecular cloning and characterization of human podocalyxin-like protein. Orthologous relationship to rabbit PCLP1 and rat podocalyxin..  The Journal of biological chemistry,    (20):   [PMID:9188463]
3. Schopperle, W Michael WM, Kershaw, David B DB and DeWolf, William C WC..  (2003)  Human embryonal carcinoma tumor antigen, Gp200/GCTM-2, is podocalyxin..  Biochemical and biophysical research communications,    (10):   [PMID:12504081]
4. Hillier, Ladeana W LW and 106 more authors..  (2003)  The DNA sequence of human chromosome 7..  Nature,    (10):   [PMID:12853948]
5. Sizemore, Steven S, Cicek, Muzaffer M, Sizemore, Nywana N, Ng, Kwok Peng KP and Casey, Graham G..  (2007)  Podocalyxin increases the aggressive phenotype of breast and prostate cancer cells in vitro through its interaction with ezrin..  Cancer research,    (1):   [PMID:17616675]
6. Larrucea, Susana S and 5 more authors..  (2008)  Expression of podocalyxin enhances the adherence, migration, and intercellular communication of cells..  Experimental cell research,    (10):   [PMID:18456258]
7. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,    (15):   [PMID:21406692]

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