A synthetic peptide derived from human PODXL (AA 300-479).
Positive Control
WB: HEK293, Raji, HeLa and HT-29 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human PODXL Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.6 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant PODXL Antibody (Ab122517) - Western Blot All lanes: Recombinant PODXL Antibody (Ab122517) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 59 kDa Observed band size: 164,183 kDa Exposure time: 76.5 s
Recombinant PODXL Antibody (Ab122517) - Western Blot All lanes: Recombinant PODXL Antibody (Ab122517) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 59 kDa Observed band size: 164, 183 kDa Exposure time: 28.3 s
1.Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al.. (2003) Human chromosome 7: DNA sequence and biology.. Science, 300 (5620):(767-72). [PMID:12690205]
2.Kershaw, D B DB and 6 more authors.. (1997) Molecular cloning and characterization of human podocalyxin-like protein. Orthologous relationship to rabbit PCLP1 and rat podocalyxin.. The Journal of biological chemistry, (20):[PMID:9188463]
3.Schopperle, W Michael WM, Kershaw, David B DB and DeWolf, William C WC.. (2003) Human embryonal carcinoma tumor antigen, Gp200/GCTM-2, is podocalyxin.. Biochemical and biophysical research communications, (10):[PMID:12504081]
4.Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10):[PMID:12853948]
5.Sizemore, Steven S, Cicek, Muzaffer M, Sizemore, Nywana N, Ng, Kwok Peng KP and Casey, Graham G.. (2007) Podocalyxin increases the aggressive phenotype of breast and prostate cancer cells in vitro through its interaction with ezrin.. Cancer research, (1):[PMID:17616675]
6.Larrucea, Susana S and 5 more authors.. (2008) Expression of podocalyxin enhances the adherence, migration, and intercellular communication of cells.. Experimental cell research, (10):[PMID:18456258]
7.Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15):[PMID:21406692]
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