Recombinant POLR2E/rpb5 Antibody - Primary antibody, specific to POLR2E, Rabbit IgG

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab122593
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab122593-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$99.90
Ab122593-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab122593-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$399.90
Ab122593-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Recombinant; Rabbit anti Human POLR2E/rpb5 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameRecombinant POLR2E/rpb5 Antibody - Primary antibody, specific to POLR2E, Rabbit IgG
SynonymsRPB5 | XAP4 | RPABC1 | hRPB25 | hsRPB5 | and III subunit ABC1 antibody | and III subunit RPABC1 antibody | DNA directed RNA polymerase II 23 kda polypeptide antibody | DNA directed RNA polymerase II polypeptide E antibody | DNA directed RNA polymerase II
Specifications & PurityExactAb™, Validated, Recombinant, 0.3 mg/mL
Host speciesRabbit
SpecificityPOLR2E
ImmunogenA synthetic peptide derived from human POLR2E (AA 41-54)
Positive ControlWB: K562, HeLa, A431, Hep G2, U937, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human POLR2E/rpb5 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.3 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant POLR2E/rpb5 Antibody (Ab122593) - Western Blot
All lanes: Recombinant POLR2E/rpb5 Antibody (Ab122593) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 27 kDa
Exposure time: 1.0 s


Recombinant POLR2E/rpb5 Antibody (Ab122593) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant POLR2E/rpb5 Antibody (Ab122593). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

POLR2E Tbio DNA-directed RNA polymerases I, II, and III subunit RPABC1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IHC1/50 - 1/100

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1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0606488Certificate of AnalysisJun 04, 2024 Ab122593
ZJ24F0606489Certificate of AnalysisJun 04, 2024 Ab122593

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