Recombinant Proteasome 20S alpha 2/HC3 Antibody - Primary antibody, specific to Proteasome 20S alpha 2/HC3, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab123373
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab123373-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab123373-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab123373-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab123373-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Recombinant; Rabbit anti Human Proteasome 20S alpha 2/HC3 Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant Proteasome 20S alpha 2/HC3 Antibody - Primary antibody, specific to Proteasome 20S alpha 2/HC3, Rabbit IgG
SynonymsMacropain subunit C3 | Multicatalytic endopeptidase complex subunit C3 | Proteasome component C3 | HC3 antibody | Macropain subunit C3 antibody | MU antibody | Multicatalytic endopeptidase complex subunit C3 antibody | PMSA2 antibody | Proteasome (prosome
Specifications & PurityExactAb™, Validated, Recombinant, 0.2 mg/mL
Host speciesRabbit
SpecificityProteasome 20S alpha 2/HC3
ImmunogenA synthetic peptide derived from human Proteasome 20S alpha 2 (AA 58-69)
Positive ControlWB: K562, HeLa, A549, Hep G2 and NIH/3T3 cell lysates. IHC: Human tonsil tissue. ICC/IF: U87-MG cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Proteasome 20S alpha 2/HC3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.2 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Proteasome 20S alpha 2/HC3 Antibody (Ab123373) - Western Blot
All lanes: Recombinant Proteasome 20S alpha 2/HC3 Antibody (Ab123373) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 25 kDa
Exposure time: 100.0 s 

Recombinant Proteasome 20S alpha 2/HC3 Antibody (Ab123373) - Western Blot
All lanes: Recombinant Proteasome 20S alpha 2/HC3 Antibody (Ab123373) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 25 kDa
Exposure time: 48.6 s 

Recombinant Proteasome 20S alpha 2/HC3 Antibody (Ab123373) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant Proteasome 20S alpha 2/HC3 Antibody (Ab123373). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Recombinant Proteasome 20S alpha 2/HC3 Antibody (Ab123373) - IF/ICC
Immunocytochemistry analysis of Proteasome alpha 2 (green) in U87-MG using Recombinant Proteasome 20S alpha 2/HC3 Antibody (Ab123373) and DAPI (blue).

Associated Targets(Human)

PSMA2 Tbio Proteasome subunit alpha type-2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/1000

">

1/500-1/1000

IF/ICC 1/50-1/200

">

1/50-1/200

IHC1/50 - 1/100

">

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0606492Certificate of AnalysisJun 04, 2024 Ab123373
ZJ24F0606493Certificate of AnalysisJun 04, 2024 Ab123373

Related Documents

References

1. Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al..  (2003)  Human chromosome 7: DNA sequence and biology..  Science,  300  (5620): (767-72).  [PMID:12690205] [10.1021/op500134e]
2. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861] [10.1021/op500134e]
3. Fan H, Angelo NG, Warren JD, Nathan CF, Lin G.  (2014)  Oxathiazolones Selectively Inhibit the Human Immunoproteasome over the Constitutive Proteasome..  ACS Med Chem Lett,  (4): (405-10).  [PMID:24900849] [10.1021/op500134e]
4. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
5. Giżyńska M, Witkowska J, Karpowicz P, Rostankowski R, Chocron ES, Pickering AM, Osmulski P, Gaczynska M, Jankowska E..  (2019)  Proline- and Arginine-Rich Peptides as Flexible Allosteric Modulators of Human Proteasome Activity..  J Med Chem,  62  (1): (359-370).  [PMID:30452262] [10.1021/acs.jmedchem.8b01025]
6. Long MJC, Lawson AP, Baggio R, Qian Y, Rozhansky L, Fasci D, El Oualid F, Weerapana E, Hedstrom L..  (2019)  Diarylcarbonates are a new class of deubiquitinating enzyme inhibitor..  Bioorg Med Chem Lett,  29  (2): (204-211).  [PMID:30528168] [10.1016/j.bmcl.2018.11.055]
7. Zhang G,Li S,Wang F,Jones AC,Goldberg AFG,Lin B,Virgil S,Stoltz BM,Deshaies RJ,Chou TF.  (2021)  A covalent p97/VCP ATPase inhibitor can overcome resistance to CB-5083 and NMS-873 in colorectal cancer cells..  Eur J Med Chem,  213  (113148-113148).  [PMID:33476933] [10.1016/j.ejmech.2020.113148]
8. Fiolek TJ,Magyar CL,Wall TJ,Davies SB,Campbell MV,Savich CJ,Tepe JJ,Mosey RA.  (2021)  Dihydroquinazolines enhance 20S proteasome activity and induce degradation of α-synuclein, an intrinsically disordered protein associated with neurodegeneration..  Bioorg Med Chem Lett,  36  (127821-127821).  [PMID:33513387] [10.1016/j.bmcl.2021.127821]
9. Tamura, T T and 7 more authors..  (1991)  Molecular cloning and sequence analysis of cDNAs for five major subunits of human proteasomes (multi-catalytic proteinase complexes)..  Biochimica et biophysica acta,    (2):   [PMID:2025653]
10. Kristensen, P P, Johnsen, A H AH, Uerkvitz, W W, Tanaka, K K and Hendil, K B KB..  (1994)  Human proteasome subunits from 2-dimensional gels identified by partial sequencing..  Biochemical and biophysical research communications,    (30):   [PMID:7811265]
11. Groettrup, M M and 8 more authors..  (1996)  A role for the proteasome regulator PA28alpha in antigen presentation..  Nature,    (9):   [PMID:8610016]
12. Takabe, W W and 9 more authors..  (2000)  Gene expression induced by BO-653, probucol and BHQ in human endothelial cells..  Journal of atherosclerosis and thrombosis,      [PMID:11521686]
13. Rush, John J and 9 more authors..  (2005)  Immunoaffinity profiling of tyrosine phosphorylation in cancer cells..  Nature biotechnology,      [PMID:15592455]
14. Leong, Wai Fook WF and Chow, Vincent T K VT..  (2006)  Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection..  Cellular microbiology,      [PMID:16548883]
15. Sjöblom, Tobias T and 28 more authors..  (2006)  The consensus coding sequences of human breast and colorectal cancers..  Science (New York, N.Y.),    (13):   [PMID:16959974]
16. Wang, Xiaorong X and 5 more authors..  (2007)  Mass spectrometric characterization of the affinity-purified human 26S proteasome complex..  Biochemistry,    (20):   [PMID:17323924]
17. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
18. Harshbarger, Wayne W, Miller, Chase C, Diedrich, Chandler C and Sacchettini, James J..  (2015)  Crystal structure of the human 20S proteasome in complex with carfilzomib..  Structure (London, England : 1993),    (3):   [PMID:25599644]

Solution Calculators