| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab123697-10μl | 10μl | In stock | $69.90 | |
| Ab123697-50μl | 50μl | In stock | $189.90 | |
| Ab123697-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab123697-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human PSMD14 Antibody; WB; Unconjugated
| Product Name | Recombinant PSMD14 Antibody - Primary antibody, specific to PSMD14, Rabbit IgG |
|---|---|
| Synonyms | 26S proteasome non-ATPase regulatory subunit 14 antibody | 26S proteasome regulatory subunit rpn11 antibody | 26S proteasome-associated PAD1 homolog 1 antibody | 26S proteasome-associated PAD1 homolog antibody | PAD1 antibody | PAD1, yeast, homolog of ant |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | PSMD14 |
| Immunogen | A synthetic peptide derived from human PSMD14 (AA 150-200) |
| Positive Control | WB: HeLa, K562, LNCaP, U2OS, SK-OV-3, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human PSMD14 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant PSMD14 Antibody (Ab123697) - Western Blot
All lanes: Recombinant PSMD14 Antibody (Ab123697) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 34 kDa
Exposure time: 53.2 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505550 | Certificate of Analysis | May 14, 2024 | Ab123697 |
| ZJ24F0505551 | Certificate of Analysis | May 14, 2024 | Ab123697 |
| 1. Fan H, Angelo NG, Warren JD, Nathan CF, Lin G. (2014) Oxathiazolones Selectively Inhibit the Human Immunoproteasome over the Constitutive Proteasome.. ACS Med Chem Lett, 5 (4): (405-10). [PMID:24900849] [10.1021/op500134e] |
| 2. Perez C, Li J, Parlati F, Rouffet M, Ma Y, Mackinnon AL, Chou TF, Deshaies RJ, Cohen SM.. (2017) Discovery of an Inhibitor of the Proteasome Subunit Rpn11.. J Med Chem, 60 (4): (1343-1361). [PMID:28191850] [10.1021/acs.jmedchem.6b01379] |
| 3. Long MJC, Lawson AP, Baggio R, Qian Y, Rozhansky L, Fasci D, El Oualid F, Weerapana E, Hedstrom L.. (2019) Diarylcarbonates are a new class of deubiquitinating enzyme inhibitor.. Bioorg Med Chem Lett, 29 (2): (204-211). [PMID:30528168] [10.1016/j.bmcl.2018.11.055] |
| 4. Kanayama, H O HO and 7 more authors.. (1992) Demonstration that a human 26S proteolytic complex consists of a proteasome and multiple associated protein components and hydrolyzes ATP and ubiquitin-ligated proteins by closely linked mechanisms.. European journal of biochemistry, (1): [PMID:1317798] |
| 5. Spataro, V V and 6 more authors.. (1997) Resistance to diverse drugs and ultraviolet light conferred by overexpression of a novel human 26 S proteasome subunit.. The Journal of biological chemistry, (28): [PMID:9374539] |
| 6. Wang, Xiaorong X and 5 more authors.. (2007) Mass spectrometric characterization of the affinity-purified human 26S proteasome complex.. Biochemistry, (20): [PMID:17323924] |
| 7. Cooper, Eric M EM and 5 more authors.. (2009) K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1.. The EMBO journal, (18): [PMID:19214193] |
| 8. Andersen, Katrine M KM and 6 more authors.. (2009) Thioredoxin Txnl1/TRP32 is a redox-active cofactor of the 26 S proteasome.. The Journal of biological chemistry, (29): [PMID:19349277] |
| 9. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 10. Butler, Laura R LR and 9 more authors.. (2012) The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response.. The EMBO journal, (3): [PMID:22909820] |
| 11. Li, Jing J and 21 more authors.. (2017) Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11.. Nature chemical biology, [PMID:28244987] |
