Rabbit anti Human PSMD14 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function: Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant PSMD14 Antibody (Ab123697) - Western Blot All lanes: Recombinant PSMD14 Antibody (Ab123697) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 35 kDa Observed band size: 34 kDa Exposure time: 53.2 s
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2.Perez C, Li J, Parlati F, Rouffet M, Ma Y, Mackinnon AL, Chou TF, Deshaies RJ, Cohen SM.. (2017) Discovery of an Inhibitor of the Proteasome Subunit Rpn11.. J Med Chem, 60 (4):(1343-1361). [PMID:28191850]
3.Long MJC, Lawson AP, Baggio R, Qian Y, Rozhansky L, Fasci D, El Oualid F, Weerapana E, Hedstrom L.. (2019) Diarylcarbonates are a new class of deubiquitinating enzyme inhibitor.. Bioorg Med Chem Lett, 29 (2):(204-211). [PMID:30528168]
4.Kanayama, H O HO and 7 more authors.. (1992) Demonstration that a human 26S proteolytic complex consists of a proteasome and multiple associated protein components and hydrolyzes ATP and ubiquitin-ligated proteins by closely linked mechanisms.. European journal of biochemistry, (1):[PMID:1317798]
5.Spataro, V V and 6 more authors.. (1997) Resistance to diverse drugs and ultraviolet light conferred by overexpression of a novel human 26 S proteasome subunit.. The Journal of biological chemistry, (28):[PMID:9374539]
6.Wang, Xiaorong X and 5 more authors.. (2007) Mass spectrometric characterization of the affinity-purified human 26S proteasome complex.. Biochemistry, (20):[PMID:17323924]
7.Cooper, Eric M EM and 5 more authors.. (2009) K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1.. The EMBO journal, (18):[PMID:19214193]
8.Andersen, Katrine M KM and 6 more authors.. (2009) Thioredoxin Txnl1/TRP32 is a redox-active cofactor of the 26 S proteasome.. The Journal of biological chemistry, (29):[PMID:19349277]
9.Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18):[PMID:19690332]
10.Butler, Laura R LR and 9 more authors.. (2012) The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response.. The EMBO journal, (3):[PMID:22909820]
11.Li, Jing J and 21 more authors.. (2017) Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11.. Nature chemical biology, [PMID:28244987]
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