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Recombinant PSMD14 Antibody - Primary antibody, specific to PSMD14, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.5 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human PSMD14 Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab123697
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab123697-10μl
10μl
In stock
$69.90
Ab123697-50μl
50μl
In stock
$189.90
Ab123697-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab123697-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Recombinant; Rabbit anti Human PSMD14 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant PSMD14 Antibody - Primary antibody, specific to PSMD14, Rabbit IgG
Synonyms26S proteasome non-ATPase regulatory subunit 14 antibody | 26S proteasome regulatory subunit rpn11 antibody | 26S proteasome-associated PAD1 homolog 1 antibody | 26S proteasome-associated PAD1 homolog antibody | PAD1 antibody | PAD1, yeast, homolog of ant
Specifications & PurityExactAb™, Validated, Recombinant, 0.5 mg/mL
Host speciesRabbit
SpecificityPSMD14
ImmunogenA synthetic peptide derived from human PSMD14 (AA 150-200)
Positive ControlWB: HeLa, K562, LNCaP, U2OS, SK-OV-3, NIH/3T3 and C6 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human PSMD14 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function:
Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant PSMD14 Antibody (Ab123697) - Western Blot
All lanes: Recombinant PSMD14 Antibody (Ab123697) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 34 kDa
Exposure time: 53.2 s


Associated Targets

PSMD14 Tchem 26S proteasome non-ATPase regulatory subunit 14 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505550Certificate of AnalysisMay 14, 2024 Ab123697
ZJ24F0505551Certificate of AnalysisMay 14, 2024 Ab123697

Related Documents

References

1. Fan H, Angelo NG, Warren JD, Nathan CF, Lin G.  (2014)  Oxathiazolones Selectively Inhibit the Human Immunoproteasome over the Constitutive Proteasome..  ACS Med Chem Lett,  (4): (405-10).  [PMID:24900849]
2. Perez C, Li J, Parlati F, Rouffet M, Ma Y, Mackinnon AL, Chou TF, Deshaies RJ, Cohen SM..  (2017)  Discovery of an Inhibitor of the Proteasome Subunit Rpn11..  J Med Chem,  60  (4): (1343-1361).  [PMID:28191850]
3. Long MJC, Lawson AP, Baggio R, Qian Y, Rozhansky L, Fasci D, El Oualid F, Weerapana E, Hedstrom L..  (2019)  Diarylcarbonates are a new class of deubiquitinating enzyme inhibitor..  Bioorg Med Chem Lett,  29  (2): (204-211).  [PMID:30528168]
4. Kanayama, H O HO and 7 more authors..  (1992)  Demonstration that a human 26S proteolytic complex consists of a proteasome and multiple associated protein components and hydrolyzes ATP and ubiquitin-ligated proteins by closely linked mechanisms..  European journal of biochemistry,    (1):   [PMID:1317798]
5. Spataro, V V and 6 more authors..  (1997)  Resistance to diverse drugs and ultraviolet light conferred by overexpression of a novel human 26 S proteasome subunit..  The Journal of biological chemistry,    (28):   [PMID:9374539]
6. Wang, Xiaorong X and 5 more authors..  (2007)  Mass spectrometric characterization of the affinity-purified human 26S proteasome complex..  Biochemistry,    (20):   [PMID:17323924]
7. Cooper, Eric M EM and 5 more authors..  (2009)  K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1..  The EMBO journal,    (18):   [PMID:19214193]
8. Andersen, Katrine M KM and 6 more authors..  (2009)  Thioredoxin Txnl1/TRP32 is a redox-active cofactor of the 26 S proteasome..  The Journal of biological chemistry,    (29):   [PMID:19349277]
9. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
10. Butler, Laura R LR and 9 more authors..  (2012)  The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response..  The EMBO journal,    (3):   [PMID:22909820]
11. Li, Jing J and 21 more authors..  (2017)  Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11..  Nature chemical biology,      [PMID:28244987]

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