Recombinant; Rabbit anti Human PU.1/Spi1 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab123929-10μl | 10μl | In stock | $69.90 | |
| Ab123929-50μl | 50μl | In stock | $189.90 | |
| Ab123929-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab123929-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human PU.1/Spi1 Antibody; WB; Unconjugated
| Product Name | Recombinant PU.1/Spi1 Antibody - Primary antibody, specific to PU.1/Spi1, Rabbit IgG |
|---|---|
| Synonyms | transcription factor spi1 antibody | 31 kDa Transforming Protein antibody | 31 kDa-transforming protein antibody | cb1086 antibody | Hematopoietic transcription factor PU.1 antibody | OFantibody | oncogene spi1 antibody | PU.1 antibody | SFFV virus-induce |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | PU.1/Spi1 |
| Immunogen | A synthetic peptide derived from human PU.1/Spi1 (AA 25-38) |
| Positive Control | WB: K562, THP-1, Ramos, U937, RAW 264.7 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human PU.1/Spi1 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant PU.1/Spi1 Antibody (Ab123929) - Western Blot
All lanes: Recombinant PU.1/Spi1 Antibody (Ab123929) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 35, 37 kDa
Exposure time: 54.3 s
Recombinant PU.1/Spi1 Antibody (Ab123929) - Western Blot
All lanes: Recombinant PU.1/Spi1 Antibody (Ab123929) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 35 kDa
Exposure time: 74.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303551 | Certificate of Analysis | Mar 27, 2024 | Ab123929 |
| ZJ24F0303550 | Certificate of Analysis | Mar 21, 2024 | Ab123929 |
| 1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 2. Ray, D D, Culine, S S, Tavitain, A A and Moreau-Gachelin, F F.. (1990) The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors.. Oncogene, [PMID:1693183] |
| 3. Rao, S S, Matsumura, A A, Yoon, J J and Simon, M C MC.. (1999) SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1.. The Journal of biological chemistry, (16): [PMID:10196196] |
| 4. Mao, S S, Frank, R C RC, Zhang, J J, Miyazaki, Y Y and Nimer, S D SD.. (1999) Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias.. Molecular and cellular biology, [PMID:10207087] |
| 5. Dahl, Richard R, Iyer, Sangeeta R SR, Owens, Kristin S KS, Cuylear, Dorothy D DD and Simon, M Celeste MC.. (2007) The transcriptional repressor GFI-1 antagonizes PU.1 activity through protein-protein interaction.. The Journal of biological chemistry, (2): [PMID:17197705] |
| 6. Basso, Katia K and 6 more authors.. (2012) BCL6 positively regulates AID and germinal center gene expression via repression of miR-155.. The Journal of experimental medicine, (17): [PMID:23166356] |
| 7. Wada, Taizo T and 8 more authors.. (2015) A Novel In-Frame Deletion in the Leucine Zipper Domain of C/EBPε Leads to Neutrophil-Specific Granule Deficiency.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:26019275] |
