| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab123953-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab123953-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $179.90 | |
| Ab123953-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $279.90 | |
| Ab123953-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human Pumilio 1 Antibody; WB; Unconjugated
| Product Name | Recombinant Pumilio 1 Antibody - Primary antibody, specific to Pumilio 1, Rabbit IgG |
|---|---|
| Synonyms | CHBL2 antibody | DCIP antibody | Dendritic cell derived IFNG induced protein antibody | Dendritic cell-derived IFNG-induced protein antibody | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody | dNTPase antibody | HD domain containing 1 ant |
| Specifications & Purity | Recombinant, ExactAb™, Validated, See COA |
| Host species | Rabbit |
| Specificity | Pumilio 1 |
| Immunogen | A synthetic peptide derived from human Pumilio 1 (AA 140-200) |
| Positive Control | WB: HeLa, K562, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Pumilio 1 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Pumilio 1 Antibody (Ab123953) - Western Blot
All lanes: Recombinant Pumilio 1 Antibody (Ab123953) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 126 kDa
Observed band size: 137 kDa
Exposure time: 1.8 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/2000"> 1/1000 - 1/2000 |
Enter Lot Number to search for COA:
| 1. Nagase, T T and 9 more authors.. (1995) Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:7788527] |
| 2. Wang, X X, Zamore, P D PD and Hall, T M TM.. (2001) Crystal structure of a Pumilio homology domain.. Molecular cell, [PMID:11336708] |
| 3. Wang, Xiaoqiang X, McLachlan, Juanita J, Zamore, Phillip D PD and Hall, Traci M Tanaka TM.. (2002) Modular recognition of RNA by a human pumilio-homology domain.. Cell, (23): [PMID:12202039] |
| 4. Spassov, Danislav S DS and Jurecic, Roland R.. (2002) Cloning and comparative sequence analysis of PUM1 and PUM2 genes, human members of the Pumilio family of RNA-binding proteins.. Gene, (16): [PMID:12459267] |
| 5. Dazard, Jean-Eudes JE and 5 more authors.. (2003) Genome-wide comparison of human keratinocyte and squamous cell carcinoma responses to UVB irradiation: implications for skin and epithelial cancer.. Oncogene, (15): [PMID:12771951] |
| 6. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 7. Gupta, Yogesh K YK, Nair, Deepak T DT, Wharton, Robin P RP and Aggarwal, Aneel K AK.. (2008) Structures of human Pumilio with noncognate RNAs reveal molecular mechanisms for binding promiscuity.. Structure (London, England : 1993), [PMID:18328718] |
| 8. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 9. Galgano, Alessia A and 5 more authors.. (2008) Comparative analysis of mRNA targets for human PUF-family proteins suggests extensive interaction with the miRNA regulatory system.. PloS one, (8): [PMID:18776931] |
| 10. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 11. Kedde, Martijn M and 5 more authors.. (2010) A Pumilio-induced RNA structure switch in p27-3' UTR controls miR-221 and miR-222 accessibility.. Nature cell biology, [PMID:20818387] |
| 12. Lu, Gang G and Hall, Traci M Tanaka TM.. (2011) Alternate modes of cognate RNA recognition by human PUMILIO proteins.. Structure (London, England : 1993), (9): [PMID:21397187] |
| 13. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 14. Filipovska, Aleksandra A, Razif, Muhammad F M MF, Nygård, Karoline K A KK and Rackham, Oliver O.. (2011) A universal code for RNA recognition by PUF proteins.. Nature chemical biology, (15): [PMID:21572425] |
| 15. Dong, Shuyun S and 8 more authors.. (2011) Specific and modular binding code for cytosine recognition in Pumilio/FBF (PUF) RNA-binding domains.. The Journal of biological chemistry, (29): [PMID:21653694] |
| 16. Miles, Wayne O WO, Tschöp, Katrin K, Herr, Anabel A, Ji, Jun-Yuan JY and Dyson, Nicholas J NJ.. (2012) Pumilio facilitates miRNA regulation of the E2F3 oncogene.. Genes & development, (15): [PMID:22345517] |
| 17. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 18. Van Etten, Jamie J and 6 more authors.. (2012) Human Pumilio proteins recruit multiple deadenylases to efficiently repress messenger RNAs.. The Journal of biological chemistry, (19): [PMID:22955276] |
| 19. Loedige, Inga I, Gaidatzis, Dimos D, Sack, Ragna R, Meister, Gunter G and Filipowicz, Witold W.. (2013) The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function.. Nucleic acids research, (7): [PMID:23125361] |
| 20. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315] |
| 21. Narita, Ryo R and 7 more authors.. (2014) A novel function of human Pumilio proteins in cytoplasmic sensing of viral infection.. PLoS pathogens, [PMID:25340845] |
| 22. Gennarino, Vincenzo A VA and 13 more authors.. (2015) Pumilio1 haploinsufficiency leads to SCA1-like neurodegeneration by increasing wild-type Ataxin1 levels.. Cell, (12): [PMID:25768905] |
| 23. Lee, Sungyul S and 8 more authors.. (2016) Noncoding RNA NORAD Regulates Genomic Stability by Sequestering PUMILIO Proteins.. Cell, (14): [PMID:26724866] |
| 24. Treiber, Thomas T and 9 more authors.. (2017) A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.. Molecular cell, (20): [PMID:28431233] |
| 25. Gennarino, Vincenzo A VA and 40 more authors.. (2018) A Mild PUM1 Mutation Is Associated with Adult-Onset Ataxia, whereas Haploinsufficiency Causes Developmental Delay and Seizures.. Cell, (22): [PMID:29474920] |
