Recombinant; Rabbit anti Human Rab5A Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab124226-10μl | 10μl | In stock | $66.90 | |
| Ab124226-50μl | 50μl | In stock | $189.90 | |
| Ab124226-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab124226-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human Rab5A Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant Rab5A Antibody - Primary antibody, specific to Rab5A, Rabbit IgG |
|---|---|
| Synonyms | RAB 5 antibody|RAB 5A antibody|RAB5A antibody|RAB5A member RAS oncogene family antibody|RAB5A_HUMAN antibody|RAS associated protein RAB5A antibody|Ras related protein Rab 5A antibody|Ras-related protein Rab-5A antibody|RAB5A|RAB5|EC 3.6.5.2 |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.7 mg/mL |
| Host species | Rabbit |
| Specificity | Rab5A |
| Immunogen | A synthetic peptide derived from human Rab5 (AA 3-19) |
| Positive Control | WB: K562, HeLa, MCF7, HEK293, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. ICC/IF: HT-1080 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Rab5A Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Rab5A Antibody (Ab124226) - Western Blot
All lanes: Recombinant Rab5A Antibody (Ab124226) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 14.6 s
Recombinant Rab5A Antibody (Ab124226) - IF/ICC
Immunocytochemistry analysis of Rab5 (green) in HT-1080 using Recombinant Rab5A Antibody (Ab124226), and cells were counterstained with DAPI (blue).
Recombinant Rab5A Antibody (Ab124226) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant Rab5A Antibody (Ab124226). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303559 | Certificate of Analysis | Mar 21, 2024 | Ab124226 |
| ZJ24F0303558 | Certificate of Analysis | Mar 21, 2024 | Ab124226 |
| 1. Zahraoui, A A, Touchot, N N, Chardin, P P and Tavitian, A A.. (1989) The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion.. The Journal of biological chemistry, (25): [PMID:2501306] |
| 2. Farnsworth, C C CC, Seabra, M C MC, Ericsson, L H LH, Gelb, M H MH and Glomset, J A JA.. (1994) Rab geranylgeranyl transferase catalyzes the geranylgeranylation of adjacent cysteines in the small GTPases Rab1A, Rab3A, and Rab5A.. Proceedings of the National Academy of Sciences of the United States of America, (6): [PMID:7991565] |
| 3. Callaghan, J J, Nixon, S S, Bucci, C C, Toh, B H BH and Stenmark, H H.. (1999) Direct interaction of EEA1 with Rab5b.. European journal of biochemistry, (1): [PMID:10491193] |
| 4. Hoffenberg, S S and 10 more authors.. (2000) A novel membrane-anchored Rab5 interacting protein required for homotypic endosome fusion.. The Journal of biological chemistry, (11): [PMID:10818110] |
| 5. Nielsen, E E and 7 more authors.. (2000) Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain.. The Journal of cell biology, (30): [PMID:11062261] |
| 6. Tall, G G GG, Barbieri, M A MA, Stahl, P D PD and Horazdovsky, B F BF.. (2001) Ras-activated endocytosis is mediated by the Rab5 guanine nucleotide exchange activity of RIN1.. Developmental cell, [PMID:11703925] |
| 7. Zhu, Guangyu G and 5 more authors.. (2003) High resolution crystal structures of human Rab5a and five mutants with substitutions in the catalytically important phosphate-binding loop.. The Journal of biological chemistry, (24): [PMID:12433916] |
| 8. Fouraux, Michael A MA and 8 more authors.. (2004) Rabip4' is an effector of rab5 and rab4 and regulates transport through early endosomes.. Molecular biology of the cell, [PMID:14617813] |
| 9. Gauthier-Campbell, Catherine C, Bredt, David S DS, Murphy, Timothy H TH and El-Husseini, Alaa El-Din Ael-D.. (2004) Regulation of dendritic branching and filopodia formation in hippocampal neurons by specific acylated protein motifs.. Molecular biology of the cell, [PMID:14978216] |
| 10. Zhu, Guangyu G and 5 more authors.. (2004) Structural basis of Rab5-Rabaptin5 interaction in endocytosis.. Nature structural & molecular biology, [PMID:15378032] |
| 11. Hadano, Shinji S and 7 more authors.. (2004) ALS2CL, the novel protein highly homologous to the carboxy-terminal half of ALS2, binds to Rab5 and modulates endosome dynamics.. FEBS letters, (24): [PMID:15388334] |
| 12. Eathiraj, Sudharshan S, Pan, Xiaojing X, Ritacco, Christopher C and Lambright, David G DG.. (2005) Structural basis of family-wide Rab GTPase recognition by rabenosyn-5.. Nature, (21): [PMID:16034420] |
| 13. Hunker, C M CM and 5 more authors.. (2006) Rab5-activating protein 6, a novel endosomal protein with a role in endocytosis.. Biochemical and biophysical research communications, (17): [PMID:16410077] |
| 14. Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27): [PMID:16641997] |
| 15. Chi, An A and 16 more authors.. (2006) Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.. Journal of proteome research, [PMID:17081065] |
| 16. Mishra, Ashwini A, Eathiraj, Sudharshan S, Corvera, Silvia S and Lambright, David G DG.. (2010) Structural basis for Rab GTPase recognition and endosome tethering by the C2H2 zinc finger of Early Endosomal Autoantigen 1 (EEA1).. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:20534488] |
| 17. Mamo, Aline A, Jules, Felix F, Dumaresq-Doiron, Karine K, Costantino, Santiago S and Lefrancois, Stephane S.. (2012) The role of ceroid lipofuscinosis neuronal protein 5 (CLN5) in endosomal sorting.. Molecular and cellular biology, [PMID:22431521] |
| 18. Baietti, Maria Francesca MF and 11 more authors.. (2012) Syndecan-syntenin-ALIX regulates the biogenesis of exosomes.. Nature cell biology, (3): [PMID:22660413] |
| 19. Blümer, Julia J and 7 more authors.. (2013) RabGEFs are a major determinant for specific Rab membrane targeting.. The Journal of cell biology, (4): [PMID:23382462] |
| 20. Kirsten, Marie L ML, Baron, Rudi A RA, Seabra, Miguel C MC and Ces, Oscar O.. (2013) Rab1a and Rab5a preferentially bind to binary lipid compositions with higher stored curvature elastic energy.. Molecular membrane biology, [PMID:23815289] |
| 21. Nakatsu, Fubito F and 6 more authors.. (2015) Sac2/INPP5F is an inositol 4-phosphatase that functions in the endocytic pathway.. The Journal of cell biology, (13): [PMID:25869668] |
| 22. Steger, Martin M and 11 more authors.. (2017) Systematic proteomic analysis of LRRK2-mediated Rab GTPase phosphorylation establishes a connection to ciliogenesis.. eLife, (10): [PMID:29125462] |
| 23. Gan, Jiyao and 10 more authors.. (2020) The Salmonella Effector SseK3 Targets Small Rab GTPases.. Frontiers in cellular and infection microbiology, [PMID:32974215] |
