Recombinant; Rabbit anti Human RACK1 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab124329-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab124329-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab124329-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab124329-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human RACK1 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant RACK1 Antibody - Primary antibody, specific to RACK1, Rabbit IgG |
|---|---|
| Synonyms | Cell proliferation-inducing gene 21 protein antibody; GBLP_HUMAN antibody; Gnb2-rs1 antibody; Gnb2l1 antibody; Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody; Guanine nucleotide binding protein beta polypeptide 2 like 1 |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | RACK1 |
| Immunogen | A synthetic peptide derived from human RACK1 (AA 271-286) |
| Positive Control | WB: MCF7, THP-1, HEK293, Jutkat, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human RACK1 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant RACK1 Antibody (Ab124329) - Western Blot
All lanes: Recombinant RACK1 Antibody (Ab124329) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 32 kDa
Exposure time: 3.7 s
Recombinant RACK1 Antibody (Ab124329) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant RACK1 Antibody (Ab124329). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0606732 | Certificate of Analysis | Jun 11, 2024 | Ab124329 |
| ZJ24F0606733 | Certificate of Analysis | Jun 11, 2024 | Ab124329 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Elayanambi Sundaramoorthy,Marilyn Leonard,Raymond Mak,Jeffrey Liao,Amitkumar Fulzele,Eric J Bennett. (2017-01-31) ZNF598 and RACK1 Regulate Mammalian Ribosome-Associated Quality Control Function by Mediating Regulatory 40S Ribosomal Ubiquitylation.. Molecular cell, 65 ((4)): (751-760). [PMID:28132843] |
| 3. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 4. Guillemot, F F, Billault, A A and Auffray, C C.. (1989) Physical linkage of a guanine nucleotide-binding protein-related gene to the chicken major histocompatibility complex.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2499885] |
| 5. Chang, B Y BY, Conroy, K B KB, Machleder, E M EM and Cartwright, C A CA.. (1998) RACK1, a receptor for activated C kinase and a homolog of the beta subunit of G proteins, inhibits activity of src tyrosine kinases and growth of NIH 3T3 cells.. Molecular and cellular biology, [PMID:9584165] |
| 6. Baumann, M M and 6 more authors.. (2000) The PKC targeting protein RACK1 interacts with the Epstein-Barr virus activator protein BZLF1.. European journal of biochemistry, [PMID:10849009] |
| 7. Chang, B Y BY, Chiang, M M and Cartwright, C A CA.. (2001) The interaction of Src and RACK1 is enhanced by activation of protein kinase C and tyrosine phosphorylation of RACK1.. The Journal of biological chemistry, (8): [PMID:11279199] |
| 8. Gallina, A A, Rossi, F F and Milanesi, G G.. (2001) Rack1 binds HIV-1 Nef and can act as a Nef-protein kinase C adaptor.. Virology, (25): [PMID:11312657] |
| 9. Hermanto, Ulrich U, Zong, Cong S CS, Li, Weiqun W and Wang, Lu-Hai LH.. (2002) RACK1, an insulin-like growth factor I (IGF-I) receptor-interacting protein, modulates IGF-I-dependent integrin signaling and promotes cell spreading and contact with extracellular matrix.. Molecular and cellular biology, [PMID:11884618] |
| 10. Liedtke, Carole M CM, Yun, C H Chris CH, Kyle, Nicole N and Wang, Dandan D.. (2002) Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor.. The Journal of biological chemistry, (21): [PMID:11956211] |
| 11. Chang, Betty Y BY, Harte, Rachel A RA and Cartwright, Christine A CA.. (2002) RACK1: a novel substrate for the Src protein-tyrosine kinase.. Oncogene, (31): [PMID:12400005] |
| 12. Rigas, Anastasia C AC, Ozanne, Daniel M DM, Neal, David E DE and Robson, Craig N CN.. (2003) The scaffolding protein RACK1 interacts with androgen receptor and promotes cross-talk through a protein kinase C signaling pathway.. The Journal of biological chemistry, (14): [PMID:12958311] |
| 13. Guil, Sònia S and 6 more authors.. (2003) Alternative splicing of the human proto-oncogene c-H-ras renders a new Ras family protein that trafficks to cytoplasm and nucleus.. Cancer research, (1): [PMID:14500341] |
| 14. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 15. Nery, Flávia C FC, Passos, Dario O DO, Garcia, Vera S VS and Kobarg, Jörg J.. (2004) Ki-1/57 interacts with RACK1 and is a substrate for the phosphorylation by phorbol 12-myristate 13-acetate-activated protein kinase C.. The Journal of biological chemistry, (19): [PMID:14699138] |
| 16. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455] |
| 17. Arya, Ranjana R and 6 more authors.. (2004) Muscle ring finger protein-1 inhibits PKC{epsilon} activation and prevents cardiomyocyte hypertrophy.. The Journal of cell biology, (20): [PMID:15596539] |
| 18. Kraus, Sarah S, Gioeli, Daniel D, Vomastek, Tomas T, Gordon, Vicki V and Weber, Michael J MJ.. (2006) Receptor for activated C kinase 1 (RACK1) and Src regulate the tyrosine phosphorylation and function of the androgen receptor.. Cancer research, (15): [PMID:17108144] |
| 19. Chuang, N-N NN and Huang, C-C CC.. (2007) Interaction of integrin beta1 with cytokeratin 1 in neuroblastoma NMB7 cells.. Biochemical Society transactions, [PMID:17956333] |
| 20. Ohgaki, Ryuichi R, Fukura, Naomi N, Matsushita, Masafumi M, Mitsui, Keiji K and Kanazawa, Hiroshi H.. (2008) Cell surface levels of organellar Na+/H+ exchanger isoform 6 are regulated by interaction with RACK1.. The Journal of biological chemistry, (15): [PMID:18057008] |
| 21. Parent, Audrey A, Laroche, Geneviève G, Hamelin, Emilie E and Parent, Jean-Luc JL.. (2008) RACK1 regulates the cell surface expression of the G protein-coupled receptor for thromboxane A(2).. Traffic (Copenhagen, Denmark), [PMID:18088317] |
| 22. Cao, Gang G and 6 more authors.. (2008) RACK1 inhibits TRPM6 activity via phosphorylation of the fused alpha-kinase domain.. Current biology : CB, (12): [PMID:18258429] |
| 23. Bourd-Boittin, Katia K, Le Pabic, Hélène H, Bonnier, Dominique D, L'Helgoualc'h, Annie A and Théret, Nathalie N.. (2008) RACK1, a new ADAM12 interacting protein. Contribution to liver fibrogenesis.. The Journal of biological chemistry, (19): [PMID:18621736] |
| 24. Edelmann, Mariola J MJ and 8 more authors.. (2009) Structural basis and specificity of human otubain 1-mediated deubiquitination.. The Biochemical journal, (1): [PMID:18954305] |
| 25. Kiely, Patrick A PA and 6 more authors.. (2009) Phosphorylation of RACK1 on tyrosine 52 by c-Abl is required for insulin-like growth factor I-mediated regulation of focal adhesion kinase.. The Journal of biological chemistry, (24): [PMID:19423701] |
| 26. Ikebuchi, Yuki Y and 6 more authors.. (2009) Receptor for activated C-kinase 1 regulates the cellular localization and function of ABCB4.. Hepatology research : the official journal of the Japan Society of Hepatology, [PMID:19674157] |
| 27. Ruan, Yuanyuan Y and 11 more authors.. (2009) RACK1 associates with CLEC-2 and promotes its ubiquitin-proteasome degradation.. Biochemical and biophysical research communications, (11): [PMID:19785988] |
| 28. Heinrich, C C and 9 more authors.. (2010) Copine-III interacts with ErbB2 and promotes tumor cell migration.. Oncogene, (18): [PMID:20010870] |
| 29. Cao, Xi-Xi XX and 7 more authors.. (2011) RACK1 promotes breast carcinoma migration/metastasis via activation of the RhoA/Rho kinase pathway.. Breast cancer research and treatment, [PMID:20499158] |
| 30. Gonçalves, Kaliandra A KA and 6 more authors.. (2010) Solution structure of the human signaling protein RACK1.. BMC structural biology, (8): [PMID:20529362] |
| 31. Wu, Yinyuan Y and 8 more authors.. (2010) RACK1 promotes Bax oligomerization and dissociates the interaction of Bax and Bcl-XL.. Cellular signalling, [PMID:20541605] |
| 32. Schäffler, Katrin K and 6 more authors.. (2010) A stimulatory role for the La-related protein 4B in translation.. RNA (New York, N.Y.), [PMID:20573744] |
| 33. Reiner, Cindy L CL and 5 more authors.. (2010) RACK1 associates with muscarinic receptors and regulates M(2) receptor trafficking.. PloS one, (20): [PMID:20976005] |
| 34. Yang, Ruiqing R and 10 more authors.. (2011) La-related protein 4 binds poly(A), interacts with the poly(A)-binding protein MLLE domain via a variant PAM2w motif, and can promote mRNA stability.. Molecular and cellular biology, [PMID:21098120] |
| 35. Wang, Feng F and 5 more authors.. (2011) RACK1 regulates VEGF/Flt1-mediated cell migration via activation of a PI3K/Akt pathway.. The Journal of biological chemistry, (18): [PMID:21212275] |
| 36. Thorslund, Sara E SE and 9 more authors.. (2011) The RACK1 signaling scaffold protein selectively interacts with Yersinia pseudotuberculosis virulence function.. PloS one, (10): [PMID:21347310] |
| 37. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 38. Yang, Jungwoo J and 12 more authors.. (2012) Receptor for activated C kinase 1 (RACK1) inhibits function of transient receptor potential (TRP)-type channel Pkd2L1 through physical interaction.. The Journal of biological chemistry, (24): [PMID:22174419] |
| 39. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 40. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 41. Ruiz Carrillo, David D and 6 more authors.. (2012) Structure of human Rack1 protein at a resolution of 2.45 Å.. Acta crystallographica. Section F, Structural biology and crystallization communications, (1): [PMID:22869111] |
| 42. Anger, Andreas M AM and 6 more authors.. (2013) Structures of the human and Drosophila 80S ribosome.. Nature, (2): [PMID:23636399] |
| 43. Diering, Graham H; Numata, Yuka; Fan, Steven; Church, John and Numata, Masayuki.. (2013) Endosomal acidification by Na+/H+ exchanger NHE5 regulates TrkA cell-surface targeting and NGF-induced PI3K signaling.. Molecular biology of the cell, [PMID:24006492] |
| 44. Ban, Nenad N and 24 more authors.. (2014) A new system for naming ribosomal proteins.. Current opinion in structural biology, [PMID:24524803] |
| 45. Majzoub, Karim K and 12 more authors.. (2014) RACK1 controls IRES-mediated translation of viruses.. Cell, (20): [PMID:25416947] |
| 46. Nielsen, Maja Holch MH, Flygaard, Rasmus Kock RK and Jenner, Lasse Bohl LB.. (2017) Structural analysis of ribosomal RACK1 and its role in translational control.. Cellular signalling, [PMID:28161490] |
| 47. Jha, Sujata and 8 more authors.. (2017) Trans-kingdom mimicry underlies ribosome customization by a poxvirus kinase.. Nature, (29): [PMID:28636603] |
| 48. Olesen, Christina Wilkens CW and 9 more authors.. (2018) Trafficking, localization and degradation of the Na+,HCO3- co-transporter NBCn1 in kidney and breast epithelial cells.. Scientific reports, (9): [PMID:29743600] |
