Recombinant; Rabbit anti Human Rad21 Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab124351-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab124351-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $207.90 | |
| Ab124351-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $297.90 | |
| Ab124351-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,307.90 |
Recombinant; Rabbit anti Human Rad21 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant Rad21 Antibody - Primary antibody, specific to RAD21, Rabbit IgG |
|---|---|
| Synonyms | CDLS4 antibody|Double-strand-break repair protein rad21 homolog antibody|hHR21 antibody|HR21 antibody|HRAD21 antibody|KIAA0078 antibody|MCD1 antibody|Nuclear matrix protein 1 antibody|NXP-1 antibody|NXP1 antibody|Protein involved in DNA double-strand brea |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | RAD21 |
| Immunogen | A synthetic peptide derived from human Rad21 (AA 600-631). |
| Positive Control | WB: Hela, A549, K562 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cell. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Rad21 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: · WB: 1/500-1/1000 · IHC: 1/50-1/100 Protein Function： Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
|
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kD |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Rad21 Antibody (Ab124351) - Western Blot
All lanes: Recombinant Rad21 Antibody (Ab124351) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 130 kDa
Observed band size: 117 kDa
Exposure time: 5.8 s
Recombinant Rad21 Antibody (Ab124351) - ICC/IF
Immunocytochemistry analysis of Rad21 (green) in HeLa cells using Recombinant Rad21 Antibody (Ab124351) and cells were counterstained with DAPI (blue).
Recombinant Rad21 Antibody (Ab124351) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant Rad21 Antibody (Ab124351). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800849 | Certificate of Analysis | Aug 17, 2023 | Ab124351 |
| ZJ23F0800850 | Certificate of Analysis | Aug 17, 2023 | Ab124351 |
| 1. Nomura, N N and 9 more authors.. (1994) Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:7584044] |
| 2. McKay, M J MJ and 7 more authors.. (1996) Sequence conservation of the rad21 Schizosaccharomyces pombe DNA double-strand break repair gene in human and mouse.. Genomics, (1): [PMID:8812457] |
| 3. Sadano, H H, Sugimoto, H H, Sakai, F F, Nomura, N N and Osumi, T T.. (2000) NXP-1, a human protein related to Rad21/Scc1/Mcd1, is a component of the nuclear matrix.. Biochemical and biophysical research communications, (7): [PMID:10623634] |
| 4. Losada, A A, Yokochi, T T, Kobayashi, R R and Hirano, T T.. (2000) Identification and characterization of SA/Scc3p subunits in the Xenopus and human cohesin complexes.. The Journal of cell biology, (7): [PMID:10931856] |
| 5. Hoque, M T MT and Ishikawa, F F.. (2001) Human chromatid cohesin component hRad21 is phosphorylated in M phase and associated with metaphase centromeres.. The Journal of biological chemistry, (16): [PMID:11073952] |
| 6. Severin, D M DM and 7 more authors.. (2001) Novel DNA sequence variants in the hHR21 DNA repair gene in radiosensitive cancer patients.. International journal of radiation oncology, biology, physics, (1): [PMID:11483345] |
| 7. Hauf, S S, Waizenegger, I C IC and Peters, J M JM.. (2001) Cohesin cleavage by separase required for anaphase and cytokinesis in human cells.. Science (New York, N.Y.), (17): [PMID:11509732] |
| 8. Gregson, H C HC and 5 more authors.. (2001) A potential role for human cohesin in mitotic spindle aster assembly.. The Journal of biological chemistry, (14): [PMID:11590136] |
| 9. Chen, Feng F and 6 more authors.. (2002) Caspase proteolysis of the cohesin component RAD21 promotes apoptosis.. The Journal of biological chemistry, (10): [PMID:11875078] |
| 10. Sumara, Izabela I and 6 more authors.. (2002) The dissociation of cohesin from chromosomes in prophase is regulated by Polo-like kinase.. Molecular cell, [PMID:11931760] |
| 11. Hakimi, Mohamed-Ali MA and 7 more authors.. (2002) A chromatin remodelling complex that loads cohesin onto human chromosomes.. Nature, (29): [PMID:12198550] |
| 12. Pati, Debananda D, Zhang, Nenggang N and Plon, Sharon E SE.. (2002) Linking sister chromatid cohesion and apoptosis: role of Rad21.. Molecular and cellular biology, [PMID:12417729] |
| 13. Rankin, Susannah S, Ayad, Nagi G NG and Kirschner, Marc W MW.. (2005) Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates.. Molecular cell, (15): [PMID:15837422] |
| 14. Parish, Joanna L JL and 5 more authors.. (2006) The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells.. Journal of cell science, (1): [PMID:17105772] |
| 15. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 16. Shintomi, Keishi K and Hirano, Tatsuya T.. (2009) Releasing cohesin from chromosome arms in early mitosis: opposing actions of Wapl-Pds5 and Sgo1.. Genes & development, (15): [PMID:19696148] |
| 17. Bermudez, Vladimir P VP and 6 more authors.. (2012) In vitro loading of human cohesin on DNA by the human Scc2-Scc4 loader complex.. Proceedings of the National Academy of Sciences of the United States of America, (12): [PMID:22628566] |
| 18. Deardorff, Matthew A MA and 26 more authors.. (2012) RAD21 mutations cause a human cohesinopathy.. American journal of human genetics, (8): [PMID:22633399] |
| 19. Bonora, Elena E and 25 more authors.. (2015) Mutations in RAD21 disrupt regulation of APOB in patients with chronic intestinal pseudo-obstruction.. Gastroenterology, [PMID:25575569] |
| 20. Kruszka, Paul P and 34 more authors.. (2019) Cohesin complex-associated holoprosencephaly.. Brain : a journal of neurology, (23): [PMID:31334757] |
