Recombinant; Rabbit anti Human Rad50 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab124364-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab124364-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $207.90 | |
| Ab124364-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $297.90 | |
| Ab124364-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,307.90 |
Recombinant; Rabbit anti Human Rad50 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant Rad50 Antibody - Primary antibody, specific to RAD50, Rabbit IgG |
|---|---|
| Synonyms | DNA repair protein RAD50 antibody; HRAD50 antibody; HsRAD50 antibody; Mrell antibody; NBSLD antibody; Rad 50 antibody; RAD50 2antibody; rad50 antibody; RAD50 homolog (S cerevisiae) antibody; RAD50 homolog antibody; RAD50 PEN antibody; RAD50, S. cerevisiae |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | RAD50 |
| Immunogen | Recombinant protein of human Rad50 (AA 1-425). |
| Positive Control | WB: MOLT-4, Jurkat and K562 cell lysates. IHC: Human Cholangiocarcinoma. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Rad50 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kD |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Rad50 Antibody (Ab124364) - Western Blot
All lanes: Recombinant Rad50 Antibody (Ab124364) at 1/1500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 153 kDa
Observed band size: 151 kDa
Exposure time: 5.0 s
Recombinant Rad50 Antibody (Ab124364) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma tissue using Recombinant Rad50 Antibody (Ab124364). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800953 | Certificate of Analysis | Sep 04, 2023 | Ab124364 |
| ZJ23F0800954 | Certificate of Analysis | Sep 04, 2023 | Ab124364 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Dolganov, G M GM and 6 more authors.. (1996) Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair.. Molecular and cellular biology, [PMID:8756642] |
| 3. Carney, J P JP and 8 more authors.. (1998) The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response.. Cell, (1): [PMID:9590181] |
| 4. Paull, T T TT and Gellert, M M.. (1998) The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks.. Molecular cell, [PMID:9651580] |
| 5. Trujillo, K M KM, Yuan, S S SS, Lee, E Y EY and Sung, P P.. (1998) Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95.. The Journal of biological chemistry, (21): [PMID:9705271] |
| 6. Kim, K K KK and 6 more authors.. (1999) Molecular cloning and characterization of splice variants of human RAD50 gene.. Gene, (22): [PMID:10415333] |
| 7. Zhong, Q Q and 8 more authors.. (1999) Association of BRCA1 with the hRad50-hMre11-p95 complex and the DNA damage response.. Science (New York, N.Y.), (30): [PMID:10426999] |
| 8. Wang, Y Y and 5 more authors.. (2000) BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.. Genes & development, (15): [PMID:10783165] |
| 9. Zhao, S S and 13 more authors.. (2000) Functional link between ataxia-telangiectasia and Nijmegen breakage syndrome gene products.. Nature, (25): [PMID:10839544] |
| 10. Zhu, X D XD, Küster, B B, Mann, M M, Petrini, J H JH and de Lange, T T.. (2000) Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres.. Nature genetics, [PMID:10888888] |
| 11. Xiao, J J, Liu, C C CC, Chen, P L PL and Lee, W H WH.. (2001) RINT-1, a novel Rad50-interacting protein, participates in radiation-induced G(2)/M checkpoint control.. The Journal of biological chemistry, (2): [PMID:11096100] |
| 12. de Jager, M M and 5 more authors.. (2001) Human Rad50/Mre11 is a flexible complex that can tether DNA ends.. Molecular cell, [PMID:11741547] |
| 13. Stracker, Travis H TH, Carson, Christian T CT and Weitzman, Matthew D MD.. (2002) Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex.. Nature, (18): [PMID:12124628] |
| 14. de Jager, Martijn M, Wyman, Claire C, van Gent, Dik C DC and Kanaar, Roland R.. (2002) DNA end-binding specificity of human Rad50/Mre11 is influenced by ATP.. Nucleic acids research, (15): [PMID:12384589] |
| 15. Heikkinen, K K, Karppinen, S-M SM, Soini, Y Y, Mäkinen, M M and Winqvist, R R.. (2003) Mutation screening of Mre11 complex genes: indication of RAD50 involvement in breast and ovarian cancer susceptibility.. Journal of medical genetics, [PMID:14684699] |
| 16. Lee, Ji-Hoon JH and Paull, Tanya T TT.. (2004) Direct activation of the ATM protein kinase by the Mre11/Rad50/Nbs1 complex.. Science (New York, N.Y.), (2): [PMID:15064416] |
| 17. Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16): [PMID:15372022] |
| 18. Zhang, Xiaoshan X and 5 more authors.. (2004) Artemis is a phosphorylation target of ATM and ATR and is involved in the G2/M DNA damage checkpoint response.. Molecular and cellular biology, [PMID:15456891] |
| 19. Chen, Ling L and 10 more authors.. (2005) Ataxia-telangiectasia-mutated dependent phosphorylation of Artemis in response to DNA damage.. Cancer science, [PMID:15723659] |
| 20. Bhoumik, Anindita A and 5 more authors.. (2005) ATM-dependent phosphorylation of ATF2 is required for the DNA damage response.. Molecular cell, (27): [PMID:15916964] |
| 21. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332] |
| 22. Waltes, Regina R and 11 more authors.. (2009) Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder.. American journal of human genetics, [PMID:19409520] |
| 23. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 24. Lee, Kyung Yong KY and 6 more authors.. (2015) MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex.. Nature communications, (28): [PMID:26215093] |
| 25. Staples, Christopher J CJ and 12 more authors.. (2016) MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response.. Cell reports, (6): [PMID:27568553] |
