Basic Description

Product Name	Recombinant RALA Antibody - Primary antibody, specific to RALA, Rabbit IgG
Synonyms	MGC48949 antibody \| Ral a antibody \| Ral A protein antibody \| RAL antibody \| RALA antibody \| RALA_HUMAN antibody \| Ras family small GTP binding protein RALA antibody \| RAS like protein Aantibody \| Ras related protein RalA antibody \| Ras-related protein Ra
Specifications & Purity	ExactAb™, Validated, Recombinant, 1.0 mg/mL
Host species	Rabbit
Specificity	RALA
Immunogen	A synthetic peptide derived from human RalA (AA 120-206)
Positive Control	WB: MCF7, Hep G2, HeLa, MOLT-4, HEK293 and HT-29 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human RALA Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function: Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant RALA Antibody (Ab124478) - Western Blot
All lanes: Recombinant RALA Antibody (Ab124478) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa
Exposure time: 6.1 s

Associated Targets(Human)

RALA Tbio Ras-related protein Ral-A (0 Activities)

Discover Target: RALA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0405035	Certificate of Analysis	Apr 29, 2024	Ab124478
ZJ24F0405034	Certificate of Analysis	Apr 29, 2024	Ab124478

FAQs and Articles

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Protocol of Western Blotting For High Molecular Weights

Categories: Protocols

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

References

1. Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al.. (2003) Human chromosome 7: DNA sequence and biology.. Science, 300 (5620): (767-72). [PMID:12690205] [10.1021/op500134e]
2. Kinsella, B T BT, Erdman, R A RA and Maltese, W A WA.. (1991) Carboxyl-terminal isoprenylation of ras-related GTP-binding proteins encoded by rac1, rac2, and ralA.. The Journal of biological chemistry, (25): [PMID:1903399]
3. Polakis, P G PG and 5 more authors.. (1989) Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets.. The Journal of biological chemistry, (5): [PMID:2550440]
4. Chardin, P P and Tavitian, A A.. (1989) Coding sequences of human ralA and ralB cDNAs.. Nucleic acids research, (12): [PMID:2662142]
5. Jullien-Flores, V V and 8 more authors.. (1995) Bridging Ral GTPase to Rho pathways. RLIP76, a Ral effector with CDC42/Rac GTPase-activating protein activity.. The Journal of biological chemistry, (22): [PMID:7673236]
6. Rebhun, J F JF, Chen, H H and Quilliam, L A LA.. (2000) Identification and characterization of a new family of guanine nucleotide exchange factors for the ras-related GTPase Ral.. The Journal of biological chemistry, (5): [PMID:10747847]
7. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948]
8. Moskalenko, Serge S and 7 more authors.. (2003) Ral GTPases regulate exocyst assembly through dual subunit interactions.. The Journal of biological chemistry, (19): [PMID:14525976]
9. Holbourn, Kenneth P KP, Sutton, J Mark JM, Evans, Hazel R HR, Shone, Clifford C CC and Acharya, K Ravi KR.. (2005) Molecular recognition of an ADP-ribosylating Clostridium botulinum C3 exoenzyme by RalA GTPase.. Proceedings of the National Academy of Sciences of the United States of America, (12): [PMID:15809419]
10. Jin, Rongsheng R and 5 more authors.. (2005) Exo84 and Sec5 are competitive regulatory Sec6/8 effectors to the RalA GTPase.. The EMBO journal, (15): [PMID:15920473]
11. Pautsch, Alexander A, Vogelsgesang, Martin M, Tränkle, Jens J, Herrmann, Christian C and Aktories, Klaus K.. (2005) Crystal structure of the C3bot-RalA complex reveals a novel type of action of a bacterial exoenzyme.. The EMBO journal, (19): [PMID:16177825]
12. Gromley, Adam A and 8 more authors.. (2005) Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission.. Cell, (7): [PMID:16213214]
13. Falsetti, Samuel C SC and 7 more authors.. (2007) Geranylgeranyltransferase I inhibitors target RalB to inhibit anchorage-dependent growth and induce apoptosis and RalA to inhibit anchorage-independent growth.. Molecular and cellular biology, [PMID:17875936]
14. Cascone, Ilaria I and 6 more authors.. (2008) Distinct roles of RalA and RalB in the progression of cytokinesis are supported by distinct RalGEFs.. The EMBO journal, (17): [PMID:18756269]
15. Aziziyeh, Adel I AI and 7 more authors.. (2009) Dual regulation of lysophosphatidic acid (LPA1) receptor signalling by Ral and GRK.. Cellular signalling, [PMID:19306925]
16. Balasubramanian, Nagaraj N and 5 more authors.. (2010) RalA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling.. Current biology : CB, (12): [PMID:20005108]
17. Kashatus, David F DF and 5 more authors.. (2011) RALA and RALBP1 regulate mitochondrial fission at mitosis.. Nature cell biology, (7): [PMID:21822277]
18. Hiatt, Susan M SM and 32 more authors.. (2018) De novo mutations in the GTP/GDP-binding region of RALA, a RAS-like small GTPase, cause intellectual disability and developmental delay.. PLoS genetics, [PMID:30500825]
19. Okamoto, Nobuhiko N, Takata, Atsushi A, Miyake, Noriko N and Matsumoto, Naomichi N.. (2019) RALA mutation in a patient with autism spectrum disorder and Noonan syndrome-like phenotype.. Congenital anomalies, (13): [PMID:30761613]

Solution Calculators

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SKU	Size	Availability	Price
Ab124478-10μl	10μl	In stock	$69.90
Ab124478-50μl	50μl	In stock	$189.90
Ab124478-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab124478-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,599.90

Recombinant RALA Antibody - Primary antibody, specific to RALA, Rabbit IgG