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Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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SKU | Size | Availability | Price | Qty |
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Ab124484-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
Ab124484-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $189.90 | |
Ab124484-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
Ab124484-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
mAb (OTI2A6); Mouse anti Human RALBP1 Antibody; WB, IHC, IF/ICC, IP; Unconjugated
Product Name | Recombinant RALBP1 Antibody - Primary antibody, specific to RALBP1, Rabbit IgG |
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Synonyms | RLIP1 antibody | 76 kDa Ral-interacting protein antibody | 76-kDa Ral-interacting protein antibody | Dinitrophenyl S-glutathione ATPase antibody | DNP-SG ATPase antibody | Ral-interacting protein 1 antibody | Ral-interacting protein 1, 76-KD antibody | Ra |
Specifications & Purity | KO Validation, ExactAb™, Validated, See COA |
Biochemical and Physiological Mechanisms | Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of |
Host species | Mouse |
Specificity | RALBP1 |
Immunogen | Recombinant f ull length protein of human RALBP1. |
Positive Control | WB: HeLa, Hep G2, MCF7, HT-29, NIH/3T3 and C6 cell lysates. IHC: Human breast adenocarcinoma, colon adenocarcinoma, kidney, ovary adenocarcinoma, endometrium adenocarcinoma, prostate, prostate carcinoma tissues. IF/ICC: COS7 cells transiently transfected |
Conjugation | Unconjugated |
Grade | ExactAb™, KO Validation, Validated |
Product Description |
Mouse anti Human RALBP1 Antibody, Monoclonal (OTI2A6), could be used for WB, IHC, IF/ICC, IP and so on.
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Isotype | Rabbit IgG |
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SDS-PAGE | 150 kDa |
Purification Method | Protein G purified |
Form | Liquid |
Concentration | See COA |
Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
Shipped In | Ice chest + Ice pads |
Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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Application | Dilution info |
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WB |
1/1000
">
1/1000
|
IF/ICC | 1/100 "> 1/100 |
IHC | 1/50 |
Enter Lot Number to search for COA:
1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
2. Jullien-Flores, V V and 8 more authors.. (1995) Bridging Ral GTPase to Rho pathways. RLIP76, a Ral effector with CDC42/Rac GTPase-activating protein activity.. The Journal of biological chemistry, (22): [PMID:7673236] |
3. Ikeda, M M, Ishida, O O, Hinoi, T T, Kishida, S S and Kikuchi, A A.. (1998) Identification and characterization of a novel protein interacting with Ral-binding protein 1, a putative effector protein of Ral.. The Journal of biological chemistry, (9): [PMID:9422736] |
4. Jullien-Flores, V V and 6 more authors.. (2000) RLIP76, an effector of the GTPase Ral, interacts with the AP2 complex: involvement of the Ral pathway in receptor endocytosis.. Journal of cell science, [PMID:10910768] |
5. Awasthi, S S and 9 more authors.. (2000) Novel function of human RLIP76: ATP-dependent transport of glutathione conjugates and doxorubicin.. Biochemistry, (8): [PMID:10924126] |
6. Sharma, R R and 7 more authors.. (2001) RLIP76 is the major ATP-dependent transporter of glutathione-conjugates and doxorubicin in human erythrocytes.. Archives of biochemistry and biophysics, (15): [PMID:11437348] |
7. Rossé, Carine C, L'Hoste, Sébastien S, Offner, Nicolas N, Picard, André A and Camonis, Jacques J.. (2003) RLIP, an effector of the Ral GTPases, is a platform for Cdk1 to phosphorylate epsin during the switch off of endocytosis in mitosis.. The Journal of biological chemistry, (15): [PMID:12775724] |
8. Yadav, Sushma S and 7 more authors.. (2004) Identification of membrane-anchoring domains of RLIP76 using deletion mutant analyses.. Biochemistry, (28): [PMID:15610018] |
9. Zahedi, René P RP and 8 more authors.. (2008) Phosphoproteome of resting human platelets.. Journal of proteome research, [PMID:18088087] |
10. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
11. Fenwick, R Brynmor and 7 more authors.. (2010) The RalB-RLIP76 complex reveals a novel mode of ral-effector interaction.. Structure (London, England : 1993), (11): [PMID:20696399] |
12. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
13. Kashatus, David F DF and 5 more authors.. (2011) RALA and RALBP1 regulate mitochondrial fission at mitosis.. Nature cell biology, (7): [PMID:21822277] |
14. Fillatre, Jonathan J and 7 more authors.. (2012) Dynamics of the subcellular localization of RalBP1/RLIP through the cell cycle: the role of targeting signals and of protein-protein interactions.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, [PMID:22319010] |
15. Rajasekar, Karthik V; Campbell, Louise J; Nietlispach, Daniel; Owen, Darerca and Mott, Helen R.. (2013) The structure of the RLIP76 RhoGAP-Ral binding domain dyad: fixed position of the domains leads to dual engagement of small G proteins at the membrane.. Structure (London, England : 1993), (3): [PMID:24207123] |