Recombinant RALBP1 Antibody - Primary antibody, specific to RALBP1, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • IP
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: OTI2A6
  • Conjugation: Unconjugated
Item Number
Ab124484
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab124484-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab124484-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90
Ab124484-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab124484-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (OTI2A6); Mouse anti Human RALBP1 Antibody; WB, IHC, IF/ICC, IP; Unconjugated

Basic Description

Product NameRecombinant RALBP1 Antibody - Primary antibody, specific to RALBP1, Rabbit IgG
SynonymsRLIP1 antibody | 76 kDa Ral-interacting protein antibody | 76-kDa Ral-interacting protein antibody | Dinitrophenyl S-glutathione ATPase antibody | DNP-SG ATPase antibody | Ral-interacting protein 1 antibody | Ral-interacting protein 1, 76-KD antibody | Ra
Specifications & PurityKO Validation, ExactAb™, Validated, See COA
Biochemical and Physiological MechanismsCan activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of
Host speciesMouse
SpecificityRALBP1
ImmunogenRecombinant f ull length protein of human RALBP1.
Positive ControlWB: HeLa, Hep G2, MCF7, HT-29, NIH/3T3 and C6 cell lysates. IHC: Human breast adenocarcinoma, colon adenocarcinoma, kidney, ovary adenocarcinoma, endometrium adenocarcinoma, prostate, prostate carcinoma tissues. IF/ICC: COS7 cells transiently transfected
ConjugationUnconjugated
GradeExactAb™, KO Validation, Validated
Product Description

Mouse anti Human RALBP1 Antibody, Monoclonal (OTI2A6), could be used for WB, IHC, IF/ICC, IP and so on.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
ConcentrationSee COA
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

RALBP1 Tbio RalA-binding protein 1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB 1/1000

">

1/1000

IF/ICC1/100

">

1/100

IHC

1/50

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Related Documents

References

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
2. Jullien-Flores, V V and 8 more authors..  (1995)  Bridging Ral GTPase to Rho pathways. RLIP76, a Ral effector with CDC42/Rac GTPase-activating protein activity..  The Journal of biological chemistry,    (22):   [PMID:7673236]
3. Ikeda, M M, Ishida, O O, Hinoi, T T, Kishida, S S and Kikuchi, A A..  (1998)  Identification and characterization of a novel protein interacting with Ral-binding protein 1, a putative effector protein of Ral..  The Journal of biological chemistry,    (9):   [PMID:9422736]
4. Jullien-Flores, V V and 6 more authors..  (2000)  RLIP76, an effector of the GTPase Ral, interacts with the AP2 complex: involvement of the Ral pathway in receptor endocytosis..  Journal of cell science,      [PMID:10910768]
5. Awasthi, S S and 9 more authors..  (2000)  Novel function of human RLIP76: ATP-dependent transport of glutathione conjugates and doxorubicin..  Biochemistry,    (8):   [PMID:10924126]
6. Sharma, R R and 7 more authors..  (2001)  RLIP76 is the major ATP-dependent transporter of glutathione-conjugates and doxorubicin in human erythrocytes..  Archives of biochemistry and biophysics,    (15):   [PMID:11437348]
7. Rossé, Carine C, L'Hoste, Sébastien S, Offner, Nicolas N, Picard, André A and Camonis, Jacques J..  (2003)  RLIP, an effector of the Ral GTPases, is a platform for Cdk1 to phosphorylate epsin during the switch off of endocytosis in mitosis..  The Journal of biological chemistry,    (15):   [PMID:12775724]
8. Yadav, Sushma S and 7 more authors..  (2004)  Identification of membrane-anchoring domains of RLIP76 using deletion mutant analyses..  Biochemistry,    (28):   [PMID:15610018]
9. Zahedi, René P RP and 8 more authors..  (2008)  Phosphoproteome of resting human platelets..  Journal of proteome research,      [PMID:18088087]
10. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
11. Fenwick, R Brynmor and 7 more authors..  (2010)  The RalB-RLIP76 complex reveals a novel mode of ral-effector interaction..  Structure (London, England : 1993),    (11):   [PMID:20696399]
12. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,    (15):   [PMID:21406692]
13. Kashatus, David F DF and 5 more authors..  (2011)  RALA and RALBP1 regulate mitochondrial fission at mitosis..  Nature cell biology,    (7):   [PMID:21822277]
14. Fillatre, Jonathan J and 7 more authors..  (2012)  Dynamics of the subcellular localization of RalBP1/RLIP through the cell cycle: the role of targeting signals and of protein-protein interactions..  FASEB journal : official publication of the Federation of American Societies for Experimental Biology,      [PMID:22319010]
15. Rajasekar, Karthik V; Campbell, Louise J; Nietlispach, Daniel; Owen, Darerca and Mott, Helen R..  (2013)  The structure of the RLIP76 RhoGAP-Ral binding domain dyad: fixed position of the domains leads to dual engagement of small G proteins at the membrane..  Structure (London, England : 1993),    (3):   [PMID:24207123]

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