| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab124509-10μl | 10μl | In stock | $69.90 | |
| Ab124509-50μl | 50μl | In stock | $189.90 | |
| Ab124509-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab124509-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human Ran Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant Ran Antibody - Primary antibody, specific to Ran, Rabbit IgG |
|---|---|
| Synonyms | Androgen receptor associated protein 24 antibody | Androgen receptor-associated protein 24 antibody | ARA 24 antibody | ARA24 antibody | Gsp1 antibody | GTP binding nuclear protein RAN antibody | GTP-binding nuclear protein Ran antibody | GTPase Ran antib |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.2 mg/mL |
| Host species | Rabbit |
| Specificity | Ran |
| Immunogen | A synthetic peptide derived from human Ran (AA 191-204) |
| Positive Control | WB: Hep G2, HEK293, Raji, Jurkat, HeLa and RAW 264.7 cell lysates. ICC/IF: U87-MG cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Ran Antibody, Recombinant, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Ran Antibody (Ab124509) - Western Blot
All lanes: Recombinant Ran Antibody (Ab124509) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 24 kDa
Observed band size: 26 kDa
Exposure time: 10.0 s
Recombinant Ran Antibody (Ab124509) - IF/ICC
Immunocytochemistry analysis of Ran (green) in U87-MG using Recombinant Ran Antibody (Ab124509), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 "> 1/500-1/2000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404026 | Certificate of Analysis | Apr 01, 2024 | Ab124509 |
| ZJ24F0404025 | Certificate of Analysis | Apr 01, 2024 | Ab124509 |
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| 2. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 3. Steggerda SM, Black BE, Paschal BM. () Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran.. Mol Biol Cell, [PMID:10679025] [http://pubmed.ncbi.nlm.nih.gov/10679025] |
| 4. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
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| 7. Bischoff, F R FR and Ponstingl, H H.. (1991) Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1961752] |
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| 9. Scheffzek, K K, Klebe, C C, Fritz-Wolf, K K, Kabsch, W W and Wittinghofer, A A.. (1995) Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form.. Nature, (23): [PMID:7885480] |
| 10. Ren, M M and 9 more authors.. (1995) Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing.. Molecular and cellular biology, [PMID:7891706] |
| 11. Ren, M M, Drivas, G G, D'Eustachio, P P and Rush, M G MG.. (1993) Ran/TC4: a small nuclear GTP-binding protein that regulates DNA synthesis.. The Journal of cell biology, [PMID:8421051] |
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| 14. Görlich, D D, Panté, N N, Kutay, U U, Aebi, U U and Bischoff, F R FR.. (1996) Identification of different roles for RanGDP and RanGTP in nuclear protein import.. The EMBO journal, (15): [PMID:8896452] |
| 15. Fornerod, M M, Ohno, M M, Yoshida, M M and Mattaj, I W IW.. (1997) CRM1 is an export receptor for leucine-rich nuclear export signals.. Cell, (19): [PMID:9323133] |
| 16. Bischoff, F R FR and Görlich, D D.. (1997) RanBP1 is crucial for the release of RanGTP from importin beta-related nuclear transport factors.. FEBS letters, (15): [PMID:9428644] |
| 17. Ribbeck, K K, Lipowsky, G G, Kent, H M HM, Stewart, M M and Görlich, D D.. (1998) NTF2 mediates nuclear import of Ran.. The EMBO journal, (16): [PMID:9822603] |
| 18. Askjaer, P P, Jensen, T H TH, Nilsson, J J, Englmeier, L L and Kjems, J J.. (1998) The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTP.. The Journal of biological chemistry, (11): [PMID:9837918] |
| 19. Vetter, I R IR, Nowak, C C, Nishimoto, T T, Kuhlmann, J J and Wittinghofer, A A.. (1999) Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport.. Nature, (4): [PMID:10078529] |
| 20. Paraskeva, E E and 7 more authors.. (1999) CRM1-mediated recycling of snurportin 1 to the cytoplasm.. The Journal of cell biology, (19): [PMID:10209022] |
| 21. Chook, Y M YM and Blobel, G G.. (1999) Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp.. Nature, (20): [PMID:10353245] |
| 22. Vetter, I R IR, Arndt, A A, Kutay, U U, Görlich, D D and Wittinghofer, A A.. (1999) Structural view of the Ran-Importin beta interaction at 2.3 A resolution.. Cell, (28): [PMID:10367892] |
| 23. Carazo-Salas, R E RE and 5 more authors.. (1999) Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.. Nature, (8): [PMID:10408446] |
| 24. Zhang, Q H QH and 20 more authors.. (2000) Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.. Genome research, [PMID:11042152] |
| 25. Renault, L L, Kuhlmann, J J, Henkel, A A and Wittinghofer, A A.. (2001) Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1).. Cell, (20): [PMID:11336674] |
| 26. Lindsay, M E ME, Holaska, J M JM, Welch, K K, Paschal, B M BM and Macara, I G IG.. (2001) Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein export.. The Journal of cell biology, (25): [PMID:11425870] |
| 27. Englmeier, L L and 5 more authors.. (2001) RanBP3 influences interactions between CRM1 and its nuclear protein export substrates.. EMBO reports, [PMID:11571268] |
| 28. Seewald, Michael J MJ, Körner, Carolin C, Wittinghofer, Alfred A and Vetter, Ingrid R IR.. (2002) RanGAP mediates GTP hydrolysis without an arginine finger.. Nature, (7): [PMID:11832950] |
| 29. Moore, William W, Zhang, Chuanmao C and Clarke, Paul R PR.. (2002) Targeting of RCC1 to chromosomes is required for proper mitotic spindle assembly in human cells.. Current biology : CB, (20): [PMID:12194828] |
| 30. Haendeler, Judith J, Hoffmann, Jörg J, Brandes, Ralf P RP, Zeiher, Andreas M AM and Dimmeler, Stefanie S.. (2003) Hydrogen peroxide triggers nuclear export of telomerase reverse transcriptase via Src kinase family-dependent phosphorylation of tyrosine 707.. Molecular and cellular biology, [PMID:12808100] |
| 31. Zou, Yonglong Y, Lim, Seunghwan S, Lee, Kangmoon K, Deng, Xiaobing X and Friedman, Eileen E.. (2003) Serine/threonine kinase Mirk/Dyrk1B is an inhibitor of epithelial cell migration and is negatively regulated by the Met adaptor Ran-binding protein M.. The Journal of biological chemistry, (5): [PMID:14500717] |
| 32. Hakata, Yoshiyuki Y, Yamada, Masami M and Shida, Hisatoshi H.. (2003) A multifunctional domain in human CRM1 (exportin 1) mediates RanBP3 binding and multimerization of human T-cell leukemia virus type 1 Rex protein.. Molecular and cellular biology, [PMID:14612415] |
| 33. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 34. Wang, Dakun D, Li, Zaibo Z, Schoen, Susan R SR, Messing, Edward M EM and Wu, Guan G.. (2004) A novel MET-interacting protein shares high sequence similarity with RanBPM, but fails to stimulate MET-induced Ras/Erk signaling.. Biochemical and biophysical research communications, (9): [PMID:14684163] |
| 35. Petosa, Carlo C and 9 more authors.. (2004) Architecture of CRM1/Exportin1 suggests how cooperativity is achieved during formation of a nuclear export complex.. Molecular cell, (3): [PMID:15574331] |
| 36. Takeda, Eri E, Hieda, Miki M, Katahira, Jun J and Yoneda, Yoshihiro Y.. (2005) Phosphorylation of RanGAP1 stabilizes its interaction with Ran and RanBP1.. Cell structure and function, [PMID:16428860] |
| 37. Chi, An A and 16 more authors.. (2006) Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.. Journal of proteome research, [PMID:17081065] |
| 38. Yamashita, Daisuke D and 5 more authors.. (2007) Human DNA replication-related element binding factor (hDREF) self-association via hATC domain is necessary for its nuclear accumulation and DNA binding.. The Journal of biological chemistry, (9): [PMID:17209048] |
| 39. Van Impe, Katrien K and 6 more authors.. (2008) A new role for nuclear transport factor 2 and Ran: nuclear import of CapG.. Traffic (Copenhagen, Denmark), [PMID:18266911] |
| 40. Xia, Fang F, Canovas, Pedro M PM, Guadagno, Thomas M TM and Altieri, Dario C DC.. (2008) A survivin-ran complex regulates spindle formation in tumor cells.. Molecular and cellular biology, [PMID:18591255] |
| 41. Schrader, Nils N and 6 more authors.. (2008) The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex.. Structure (London, England : 1993), [PMID:18611384] |
| 42. Sanz-García, Marta M, López-Sánchez, Inmaculada I and Lazo, Pedro A PA.. (2008) Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities.. Molecular & cellular proteomics : MCP, [PMID:18617507] |
| 43. Monecke, Thomas T and 5 more authors.. (2009) Crystal structure of the nuclear export receptor CRM1 in complex with Snurportin1 and RanGTP.. Science (New York, N.Y.), (22): [PMID:19389996] |
| 44. Medendorp, Klaas K and 7 more authors.. (2009) The mitotic arrest deficient protein MAD2B interacts with the small GTPase RAN throughout the cell cycle.. PloS one, (15): [PMID:19753112] |
| 45. Koyama, Masako M and Matsuura, Yoshiyuki Y.. (2010) An allosteric mechanism to displace nuclear export cargo from CRM1 and RanGTP by RanBP1.. The EMBO journal, (16): [PMID:20485264] |
| 46. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 47. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 48. Taltynov, Oliver O and 8 more authors.. (2013) Interaction of transportin-SR2 with Ras-related nuclear protein (Ran) GTPase.. The Journal of biological chemistry, (30): [PMID:23878195] |
| 49. Maertens, Goedele N GN and 13 more authors.. (2014) Structural basis for nuclear import of splicing factors by human Transportin 3.. Proceedings of the National Academy of Sciences of the United States of America, (18): [PMID:24449914] |
| 50. Tsirkone, Vicky G VG and 5 more authors.. (2014) Structure of transportin SR2, a karyopherin involved in human disease, in complex with Ran.. Acta crystallographica. Section F, Structural biology communications, [PMID:24915079] |
| 51. Bacot-Davis, Valjean R VR, Ciomperlik, Jessica J JJ, Basta, Holly A HA, Cornilescu, Claudia C CC and Palmenberg, Ann C AC.. (2014) Solution structures of Mengovirus Leader protein, its phosphorylated derivatives, and in complex with nuclear transport regulatory protein, RanGTPase.. Proceedings of the National Academy of Sciences of the United States of America, (4): [PMID:25331866] |
| 52. Rudack, Till T and 5 more authors.. (2015) Catalysis of GTP hydrolysis by small GTPases at atomic detail by integration of X-ray crystallography, experimental, and theoretical IR spectroscopy.. The Journal of biological chemistry, (2): [PMID:26272610] |
| 53. Dörr, Angela A and 5 more authors.. (2015) MYCBP2 Is a Guanosine Exchange Factor for Ran Protein and Determines Its Localization in Neurons of Dorsal Root Ganglia.. The Journal of biological chemistry, (16): [PMID:26304119] |
| 54. Fung, Ho Yee Joyce HY, Fu, Szu-Chin SC, Brautigam, Chad A CA and Chook, Yuh Min YM.. (2015) Structural determinants of nuclear export signal orientation in binding to exportin CRM1.. eLife, (8): [PMID:26349033] |
| 55. Aksu, Metin M, Trakhanov, Sergei S and Görlich, Dirk D.. (2016) Structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A.. Nature communications, (16): [PMID:27306458] |
| 56. Fung, Ho Yee Joyce HY, Fu, Szu-Chin SC and Chook, Yuh Min YM.. (2017) Nuclear export receptor CRM1 recognizes diverse conformations in nuclear export signals.. eLife, (10): [PMID:28282025] |
| 57. Bao, Xiaoling X and 19 more authors.. (2018) Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation.. Journal of molecular cell biology, (1): [PMID:29040603] |
| 58. Piano Mortari, E E and 17 more authors.. (2018) The Vici syndrome protein EPG5 regulates intracellular nucleic acid trafficking linking autophagy to innate and adaptive immunity.. Autophagy, [PMID:29130391] |
