| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab124603-10μl | 10μl | In stock | $69.90 | |
| Ab124603-50μl | 50μl | In stock | $189.90 | |
| Ab124603-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab124603-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human RAP80 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant RAP80 Antibody - Primary antibody, specific to RAP80, Rabbit IgG |
|---|---|
| Synonyms | Receptor-associated protein 80 | Retinoid X receptor-interacting protein 110 | Ubiquitin interaction motif-containing protein 1 | BRCA1-A complex subunit RAP80 antibody | Nuclear zinc finger protein RAP80 antibody | OTTHUMP00000161441 antibody | OTTHUMP00 |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | RAP80 |
| Immunogen | A synthetic peptide derived from human RAP80 (AA 26-40) |
| Positive Control | WB: MCF7 and U2OS cell lysates. IHC: Human breast cancer tissue. ICC/IF:HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human RAP80 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant RAP80 Antibody (Ab124603) - Western Blot
All lanes: Recombinant RAP80 Antibody (Ab124603) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
Exposure time: 15.9 s
Recombinant RAP80 Antibody (Ab124603) - IF/ICC
Immunocytochemistry analysis of RAP80 (green) in HeLa using Recombinant RAP80 Antibody (Ab124603), and cells were counterstained with DAPI (blue).
Recombinant RAP80 Antibody (Ab124603) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant RAP80 Antibody (Ab124603). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404030 | Certificate of Analysis | Apr 01, 2024 | Ab124603 |
| ZJ24F0404029 | Certificate of Analysis | Apr 01, 2024 | Ab124603 |
| 1. Yan Z, Kim YS, Jetten AM. (2002) RAP80, a novel nuclear protein that interacts with the retinoid-related testis-associated receptor.. J Biol Chem, 277 (35): (32379-88). [PMID:12080054] [10.1021/op500134e] |
| 2. Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16): [PMID:15372022] |
| 3. Yan, Jun J and 5 more authors.. (2007) Ubiquitin-interaction motifs of RAP80 are critical in its regulation of estrogen receptor alpha.. Nucleic acids research, [PMID:17311814] |
| 4. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332] |
| 5. Wang, Bin B and 6 more authors.. (2007) Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response.. Science (New York, N.Y.), (25): [PMID:17525340] |
| 6. Sobhian, Bijan B and 7 more authors.. (2007) RAP80 targets BRCA1 to specific ubiquitin structures at DNA damage sites.. Science (New York, N.Y.), (25): [PMID:17525341] |
| 7. Kim, Hongtae H, Chen, Junjie J and Yu, Xiaochun X.. (2007) Ubiquitin-binding protein RAP80 mediates BRCA1-dependent DNA damage response.. Science (New York, N.Y.), (25): [PMID:17525342] |
| 8. Yan, Jun J and 6 more authors.. (2007) The ubiquitin-interacting motif containing protein RAP80 interacts with BRCA1 and functions in DNA damage repair response.. Cancer research, (15): [PMID:17621610] |
| 9. Liu, Zixing Z, Wu, Jiaxue J and Yu, Xiaochun X.. (2007) CCDC98 targets BRCA1 to DNA damage sites.. Nature structural & molecular biology, [PMID:17643121] |
| 10. Kim, Hongtae H, Huang, Jun J and Chen, Junjie J.. (2007) CCDC98 is a BRCA1-BRCT domain-binding protein involved in the DNA damage response.. Nature structural & molecular biology, [PMID:17643122] |
| 11. Yan, Jun J, Yang, Xiao-Ping XP, Kim, Yong-Sik YS, Joo, Joung Hyuck JH and Jetten, Anton M AM.. (2007) RAP80 interacts with the SUMO-conjugating enzyme UBC9 and is a novel target for sumoylation.. Biochemical and biophysical research communications, (12): [PMID:17698038] |
| 12. Wang, Bin B and Elledge, Stephen J SJ.. (2007) Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:18077395] |
| 13. Yan, Jun J, Yang, Xiao-Ping XP, Kim, Yong-Sik YS and Jetten, Anton M AM.. (2008) RAP80 responds to DNA damage induced by both ionizing radiation and UV irradiation and is phosphorylated at Ser 205.. Cancer research, (1): [PMID:18519686] |
| 14. Novak, David J DJ and 5 more authors.. (2009) Analysis of the genes coding for the BRCA1-interacting proteins, RAP80 and Abraxas (CCDC98), in high-risk, non-BRCA1/2, multiethnic breast cancer cases.. Breast cancer research and treatment, [PMID:18695986] |
| 15. Wu, Jiaxue J and 7 more authors.. (2009) Histone ubiquitination associates with BRCA1-dependent DNA damage response.. Molecular and cellular biology, [PMID:19015238] |
| 16. Shao, Genze G and 5 more authors.. (2009) The Rap80-BRCC36 de-ubiquitinating enzyme complex antagonizes RNF8-Ubc13-dependent ubiquitination events at DNA double strand breaks.. Proceedings of the National Academy of Sciences of the United States of America, (3): [PMID:19202061] |
| 17. Shao, Genze G and 6 more authors.. (2009) MERIT40 controls BRCA1-Rap80 complex integrity and recruitment to DNA double-strand breaks.. Genes & development, (15): [PMID:19261746] |
| 18. Feng, Lin L, Huang, Jun J and Chen, Junjie J.. (2009) MERIT40 facilitates BRCA1 localization and DNA damage repair.. Genes & development, (15): [PMID:19261748] |
| 19. Wang, Bin B, Hurov, Kristen K, Hofmann, Kay K and Elledge, Stephen J SJ.. (2009) NBA1, a new player in the Brca1 A complex, is required for DNA damage resistance and checkpoint control.. Genes & development, (15): [PMID:19261749] |
| 20. Sims, Joshua J JJ and Cohen, Robert E RE.. (2009) Linkage-specific avidity defines the lysine 63-linked polyubiquitin-binding preference of rap80.. Molecular cell, (27): [PMID:19328070] |
| 21. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 22. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 23. Panier, Stephanie S and 6 more authors.. (2012) Tandem protein interaction modules organize the ubiquitin-dependent response to DNA double-strand breaks.. Molecular cell, (10): [PMID:22742833] |
| 24. Zheng, Hui H and 10 more authors.. (2013) A BRISC-SHMT complex deubiquitinates IFNAR1 and regulates interferon responses.. Cell reports, (17): [PMID:24075985] |
| 25. Anamika, Markin, Craig J CJ, Rout, Manoj K MK and Spyracopoulos, Leo L.. (2014) Molecular basis for impaired DNA damage response function associated with the RAP80 ΔE81 defect.. The Journal of biological chemistry, (2): [PMID:24627472] |
| 26. Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V and Vertegaal, Alfred C O.. (2015) SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage.. Cell reports, (17): [PMID:25772364] |
| 27. Soo Lee, Nam N and 13 more authors.. (2016) TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage.. Nature communications, (19): [PMID:26781088] |
