Recombinant RBBP4 Antibody - Primary antibody, specific to RBBP4, Rabbit IgG
Synonyms
CAF-1 p48 | CAF-1 subunit C | CAF-1C | CAF-I p48 | Chromatin assembly factor 1 subunit C | Chromatin assembly factor I p48 subunit | chromatin assembly factor/CAF-1 p48 subunit | histone-binding protein RBBP4 | MSI1 protein homolog | Nucleosome-remodeling
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.6 mg/mL
Host species
Rabbit
Specificity
RBBP4
Immunogen
A synthetic peptide derived from human RbAp48 (AA 2-15)
Positive Control
WB: Mouse spleen, HeLa, Jurkat, HEK293, Hep G2, NIH/3T3 and C6 lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human RBBP4 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50-1/200
Protein Function
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.6 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant RBBP4 Antibody (Ab124746) - Western Blot All lanes: Recombinant RBBP4 Antibody (Ab124746) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 5.0 s
Recombinant RBBP4 Antibody (Ab124746) - IF/ICC Immunocytochemistry analysis of RBBP4 (green) in HeLa using Recombinant RBBP4 Antibody (Ab124746), and DAPI (blue).
Recombinant RBBP4 Antibody (Ab124746) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant RBBP4 Antibody (Ab124746). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Associated Targets(Human)
RBBP4TbioHistone-binding protein RBBP4 (0 Activities)