Recombinant; Rabbit anti Human RBX1 Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab124848-10μl | 10μl | In stock | $66.90 | |
| Ab124848-50μl | 50μl | In stock | $199.90 | |
| Ab124848-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab124848-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human RBX1 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant RBX1 Antibody - Primary antibody, specific to RBX1, Rabbit IgG |
|---|---|
| Synonyms | BA554C12.1; E3 ubiquitin-protein ligase RBX1; FLJ60363; MGC13357; MGC1481; OTTHUMP00000028983; Protein ZYP; Rbx 1; Rbx1; RBX1_HUMAN; Regulator of cullins 1; Ring box 1; Ring box 1 E3 ubiquitin protein ligase; RING box protein 1; RING finger protein 75; RI |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.7 mg/mL |
| Host species | Rabbit |
| Specificity | RBX1 |
| Immunogen | A synthetic peptide derived from human ROC1 (AA 89-108). |
| Positive Control | WB: HeLa, Jurkat, THP-1, Hep G2, HEK293, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human RBX1 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant RBX1 Antibody (Ab124848) - Western Blot
All lanes: Recombinant RBX1 Antibody (Ab124848) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 16 kDa
Exposure time: 10.0 s
Recombinant RBX1 Antibody (Ab124848) - IF/ICC
Immunocytochemistry analysis of ROC1 (green) in HeLa using Recombinant RBX1 Antibody (Ab124848), and cells were counterstained with DAPI (blue).
Recombinant RBX1 Antibody (Ab124848) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant RBX1 Antibody (Ab124848). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303569 | Certificate of Analysis | Mar 21, 2024 | Ab124848 |
| ZJ24F0303568 | Certificate of Analysis | Mar 21, 2024 | Ab124848 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Kamura, T T and 11 more authors.. (1999) Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase.. Science (New York, N.Y.), (23): [PMID:10213691] |
| 3. Tan, P P and 6 more authors.. (1999) Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha.. Molecular cell, [PMID:10230406] |
| 4. Ohta, T T, Michel, J J JJ, Schottelius, A J AJ and Xiong, Y Y.. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.. Molecular cell, [PMID:10230407] |
| 5. Kamura, T T, Conrad, M N MN, Yan, Q Q, Conaway, R C RC and Conaway, J W JW.. (1999) The Rbx1 subunit of SCF and VHL E3 ubiquitin ligase activates Rub1 modification of cullins Cdc53 and Cul2.. Genes & development, (15): [PMID:10579999] |
| 6. Dunham, I I and 24 more authors.. (1999) The DNA sequence of human chromosome 22.. Nature, (2): [PMID:10591208] |
| 7. Périn J-P and 6 more authors.. (1999) Genomic organization and expression of the ubiquitin-proteasome complex-associated protein Rbx1/ROC1/Hrt1.. Cellular and molecular biology (Noisy-le-Grand, France), [PMID:10643962] |
| 8. Furukawa, M M, Zhang, Y Y, McCarville, J J, Ohta, T T and Xiong, Y Y.. (2000) The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1.. Molecular and cellular biology, [PMID:11027288] |
| 9. Lyapina, S S and 9 more authors.. (2001) Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome.. Science (New York, N.Y.), (18): [PMID:11337588] |
| 10. Kamura, T T and 9 more authors.. (2001) Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase.. The Journal of biological chemistry, (10): [PMID:11384984] |
| 11. Zheng, Ning N and 13 more authors.. (2002) Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex.. Nature, (18): [PMID:11961546] |
| 12. Brower, Christopher S CS and 12 more authors.. (2002) Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase.. Proceedings of the National Academy of Sciences of the United States of America, (6): [PMID:12149480] |
| 13. Dias, Dora C DC, Dolios, Georgia G, Wang, Rong R and Pan, Zhen-Qiang ZQ.. (2002) CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex.. Proceedings of the National Academy of Sciences of the United States of America, (24): [PMID:12481031] |
| 14. Groisman, Regina R and 8 more authors.. (2003) The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage.. Cell, (2): [PMID:12732143] |
| 15. Wertz, Ingrid E IE and 6 more authors.. (2004) Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase.. Science (New York, N.Y.), (27): [PMID:14739464] |
| 16. Collins, John E JE and 12 more authors.. (2004) A genome annotation-driven approach to cloning the human ORFeome.. Genome biology, [PMID:15461802] |
| 17. Zhang, Donna D DD and 5 more authors.. (2005) Ubiquitination of Keap1, a BTB-Kelch substrate adaptor protein for Cul3, targets Keap1 for degradation by a proteasome-independent pathway.. The Journal of biological chemistry, (26): [PMID:15983046] |
| 18. Wang, Hengbin H and 8 more authors.. (2006) Histone H3 and H4 ubiquitylation by the CUL4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damage.. Molecular cell, (5): [PMID:16678110] |
| 19. Groisman, Regina R and 8 more authors.. (2006) CSA-dependent degradation of CSB by the ubiquitin-proteasome pathway establishes a link between complementation factors of the Cockayne syndrome.. Genes & development, (1): [PMID:16751180] |
| 20. Bhoumik, Anindita A, Singha, Netai N, O'Connell, Matthew J MJ and Ronai, Ze'ev A ZA.. (2008) Regulation of TIP60 by ATF2 modulates ATM activation.. The Journal of biological chemistry, (20): [PMID:18397884] |
| 21. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 22. Shima, Yutaka Y and 5 more authors.. (2008) PML activates transcription by protecting HIPK2 and p300 from SCFFbx3-mediated degradation.. Molecular and cellular biology, [PMID:18809579] |
| 23. Cen, Bo B, Li, Haiyang H and Weinstein, I Bernard IB.. (2009) Histidine triad nucleotide-binding protein 1 up-regulates cellular levels of p27KIP1 by targeting ScfSKP2 ubiquitin ligase and Src.. The Journal of biological chemistry, (20): [PMID:19112177] |
| 24. Huang, Danny T DT and 10 more authors.. (2009) E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.. Molecular cell, (27): [PMID:19250909] |
| 25. Isobe, Tomoyasu T and 7 more authors.. (2009) Adenovirus E1A inhibits SCF(Fbw7) ubiquitin ligase.. The Journal of biological chemistry, (9): [PMID:19679664] |
| 26. Harreman, Michelle M and 10 more authors.. (2009) Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation.. Proceedings of the National Academy of Sciences of the United States of America, (8): [PMID:19920177] |
| 27. D'Angiolella, Vincenzo V and 7 more authors.. (2010) SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation.. Nature, (1): [PMID:20596027] |
| 28. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 29. Duda, David M DM and 8 more authors.. (2012) Structure of a glomulin-RBX1-CUL1 complex: inhibition of a RING E3 ligase through masking of its E2-binding surface.. Molecular cell, (10): [PMID:22748924] |
| 30. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 31. Bae, Soo Han SH and 8 more authors.. (2013) Sestrins activate Nrf2 by promoting p62-dependent autophagic degradation of Keap1 and prevent oxidative liver damage.. Cell metabolism, (8): [PMID:23274085] |
| 32. Beck, Jochen J and 11 more authors.. (2013) Ubiquitylation-dependent localization of PLK1 in mitosis.. Nature cell biology, [PMID:23455478] |
| 33. Bommeljé, Claire C CC and 10 more authors.. (2014) Oncogenic function of SCCRO5/DCUN1D5 requires its Neddylation E3 activity and nuclear localization.. Clinical cancer research : an official journal of the American Association for Cancer Research, (15): [PMID:24192928] |
| 34. Huang, Guochang G and 10 more authors.. (2014) SCCRO3 (DCUN1D3) antagonizes the neddylation and oncogenic activity of SCCRO (DCUN1D1).. The Journal of biological chemistry, (12): [PMID:25349211] |
| 35. Genau, Heide Marika HM and 7 more authors.. (2015) CUL3-KBTBD6/KBTBD7 ubiquitin ligase cooperates with GABARAP proteins to spatially restrict TIAM1-RAC1 signaling.. Molecular cell, (19): [PMID:25684205] |
| 36. Scott, Daniel C DC and 10 more authors.. (2016) Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation.. Cell, (25): [PMID:27565346] |
| 37. Fukushima, Hidefumi H and 18 more authors.. (2017) NOTCH2 Hajdu-Cheney Mutations Escape SCFFBW7-Dependent Proteolysis to Promote Osteoporosis.. Molecular cell, (16): [PMID:29149593] |
| 38. Chen, Jie J and 6 more authors.. (2018) KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing.. Nature, [PMID:29769719] |
