Basic Description

Product Name	Recombinant RBX1 Antibody - Primary antibody, specific to RBX1, Rabbit IgG
Synonyms	BA554C12.1 \| E3 ubiquitin-protein ligase RBX1 \| FLJ60363 \| MGC13357 \| MGC1481 \| OTTHUMP00000028983 \| Protein ZYP \| Rbx 1 \| Rbx1 \| RBX1_HUMAN \| Regulator of cullins 1 \| Ring box 1 \| Ring box 1 E3 ubiquitin protein ligase \| RING box protein 1 \| RING finger
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.7 mg/mL
Host species	Rabbit
Specificity	RBX1
Immunogen	A synthetic peptide derived from human ROC1 (AA 89-108).
Positive Control	WB: HeLa, Jurkat, THP-1, Hep G2, HEK293, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human RBX1 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Immunogen affinity purified
Form	Liquid
Concentration	0.7 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant RBX1 Antibody (Ab124848) - Western Blot
All lanes: Recombinant RBX1 Antibody (Ab124848) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 12 kDa
Observed band size: 16 kDa
Exposure time: 10.0 s

Recombinant RBX1 Antibody (Ab124848) - IF/ICC
Immunocytochemistry analysis of ROC1 (green) in HeLa using Recombinant RBX1 Antibody (Ab124848), and cells were counterstained with DAPI (blue).

Recombinant RBX1 Antibody (Ab124848) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant RBX1 Antibody (Ab124848). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

MFAP4 Tbio Microfibril-associated glycoprotein 4 (0 Activities)

Discover Target: MFAP4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

RBX1 Tbio E3 ubiquitin-protein ligase RBX1 (0 Activities)

Discover Target: RBX1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000
IF/ICC	1/50-1/200 "> 1/50-1/200
IHC	1/50 - 1/100 "> 1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0303569	Certificate of Analysis	Mar 21, 2024	Ab124848
ZJ24F0303568	Certificate of Analysis	Mar 21, 2024	Ab124848

FAQs and Articles

References

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
2. Kamura, T T and 11 more authors.. (1999) Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase.. Science (New York, N.Y.), (23): [PMID:10213691]
3. Tan, P P and 6 more authors.. (1999) Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha.. Molecular cell, [PMID:10230406]
4. Ohta, T T, Michel, J J JJ, Schottelius, A J AJ and Xiong, Y Y.. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.. Molecular cell, [PMID:10230407]
5. Kamura, T T, Conrad, M N MN, Yan, Q Q, Conaway, R C RC and Conaway, J W JW.. (1999) The Rbx1 subunit of SCF and VHL E3 ubiquitin ligase activates Rub1 modification of cullins Cdc53 and Cul2.. Genes & development, (15): [PMID:10579999]
6. Dunham, I I and 24 more authors.. (1999) The DNA sequence of human chromosome 22.. Nature, (2): [PMID:10591208]
7. Périn J-P and 6 more authors.. (1999) Genomic organization and expression of the ubiquitin-proteasome complex-associated protein Rbx1/ROC1/Hrt1.. Cellular and molecular biology (Noisy-le-Grand, France), [PMID:10643962]
8. Furukawa, M M, Zhang, Y Y, McCarville, J J, Ohta, T T and Xiong, Y Y.. (2000) The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1.. Molecular and cellular biology, [PMID:11027288]
9. Lyapina, S S and 9 more authors.. (2001) Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome.. Science (New York, N.Y.), (18): [PMID:11337588]
10. Kamura, T T and 9 more authors.. (2001) Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase.. The Journal of biological chemistry, (10): [PMID:11384984]
11. Zheng, Ning N and 13 more authors.. (2002) Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex.. Nature, (18): [PMID:11961546]
12. Brower, Christopher S CS and 12 more authors.. (2002) Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase.. Proceedings of the National Academy of Sciences of the United States of America, (6): [PMID:12149480]
13. Dias, Dora C DC, Dolios, Georgia G, Wang, Rong R and Pan, Zhen-Qiang ZQ.. (2002) CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex.. Proceedings of the National Academy of Sciences of the United States of America, (24): [PMID:12481031]
14. Groisman, Regina R and 8 more authors.. (2003) The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage.. Cell, (2): [PMID:12732143]
15. Wertz, Ingrid E IE and 6 more authors.. (2004) Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase.. Science (New York, N.Y.), (27): [PMID:14739464]
16. Collins, John E JE and 12 more authors.. (2004) A genome annotation-driven approach to cloning the human ORFeome.. Genome biology, [PMID:15461802]
17. Zhang, Donna D DD and 5 more authors.. (2005) Ubiquitination of Keap1, a BTB-Kelch substrate adaptor protein for Cul3, targets Keap1 for degradation by a proteasome-independent pathway.. The Journal of biological chemistry, (26): [PMID:15983046]
18. Wang, Hengbin H and 8 more authors.. (2006) Histone H3 and H4 ubiquitylation by the CUL4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damage.. Molecular cell, (5): [PMID:16678110]
19. Groisman, Regina R and 8 more authors.. (2006) CSA-dependent degradation of CSB by the ubiquitin-proteasome pathway establishes a link between complementation factors of the Cockayne syndrome.. Genes & development, (1): [PMID:16751180]
20. Bhoumik, Anindita A, Singha, Netai N, O'Connell, Matthew J MJ and Ronai, Ze'ev A ZA.. (2008) Regulation of TIP60 by ATF2 modulates ATM activation.. The Journal of biological chemistry, (20): [PMID:18397884]
21. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976]
22. Shima, Yutaka Y and 5 more authors.. (2008) PML activates transcription by protecting HIPK2 and p300 from SCFFbx3-mediated degradation.. Molecular and cellular biology, [PMID:18809579]
23. Cen, Bo B, Li, Haiyang H and Weinstein, I Bernard IB.. (2009) Histidine triad nucleotide-binding protein 1 up-regulates cellular levels of p27KIP1 by targeting ScfSKP2 ubiquitin ligase and Src.. The Journal of biological chemistry, (20): [PMID:19112177]
24. Huang, Danny T DT and 10 more authors.. (2009) E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.. Molecular cell, (27): [PMID:19250909]
25. Isobe, Tomoyasu T and 7 more authors.. (2009) Adenovirus E1A inhibits SCF(Fbw7) ubiquitin ligase.. The Journal of biological chemistry, (9): [PMID:19679664]
26. Harreman, Michelle M and 10 more authors.. (2009) Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation.. Proceedings of the National Academy of Sciences of the United States of America, (8): [PMID:19920177]
27. D'Angiolella, Vincenzo V and 7 more authors.. (2010) SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation.. Nature, (1): [PMID:20596027]
28. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
29. Duda, David M DM and 8 more authors.. (2012) Structure of a glomulin-RBX1-CUL1 complex: inhibition of a RING E3 ligase through masking of its E2-binding surface.. Molecular cell, (10): [PMID:22748924]
30. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
31. Bae, Soo Han SH and 8 more authors.. (2013) Sestrins activate Nrf2 by promoting p62-dependent autophagic degradation of Keap1 and prevent oxidative liver damage.. Cell metabolism, (8): [PMID:23274085]
32. Beck, Jochen J and 11 more authors.. (2013) Ubiquitylation-dependent localization of PLK1 in mitosis.. Nature cell biology, [PMID:23455478]
33. Bommeljé, Claire C CC and 10 more authors.. (2014) Oncogenic function of SCCRO5/DCUN1D5 requires its Neddylation E3 activity and nuclear localization.. Clinical cancer research : an official journal of the American Association for Cancer Research, (15): [PMID:24192928]
34. Huang, Guochang G and 10 more authors.. (2014) SCCRO3 (DCUN1D3) antagonizes the neddylation and oncogenic activity of SCCRO (DCUN1D1).. The Journal of biological chemistry, (12): [PMID:25349211]
35. Genau, Heide Marika HM and 7 more authors.. (2015) CUL3-KBTBD6/KBTBD7 ubiquitin ligase cooperates with GABARAP proteins to spatially restrict TIAM1-RAC1 signaling.. Molecular cell, (19): [PMID:25684205]
36. Scott, Daniel C DC and 10 more authors.. (2016) Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation.. Cell, (25): [PMID:27565346]
37. Fukushima, Hidefumi H and 18 more authors.. (2017) NOTCH2 Hajdu-Cheney Mutations Escape SCFFBW7-Dependent Proteolysis to Promote Osteoporosis.. Molecular cell, (16): [PMID:29149593]
38. Chen, Jie J and 6 more authors.. (2018) KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing.. Nature, [PMID:29769719]

Solution Calculators

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SKU	Size	Availability	Price
Ab124848-10μl	10μl	In stock	$66.90
Ab124848-50μl	50μl	In stock	$199.90
Ab124848-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab124848-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,699.90

Recombinant RBX1 Antibody - Primary antibody, specific to RBX1, Rabbit IgG