Recombinant; Rabbit anti Human RCC1 Antibody; WB, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab124863-10μl | 10μl | In stock | $69.90 | |
| Ab124863-50μl | 50μl | In stock | $189.90 | |
| Ab124863-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab124863-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human RCC1 Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant RCC1 Antibody - Primary antibody, specific to RCC1, Rabbit IgG |
|---|---|
| Synonyms | Cell cycle regulatory protein antibody | CHC 1 antibody | CHC1 antibody | Chromosome condensation 1 antibody | Chromosome condensation protein 1 antibody | Guanine nucleotide releasing protein antibody | HERC2 antibody | Ran GEF antibody | RanGEF antibody |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | RCC1 |
| Immunogen | A synthetic peptide derived from human RCC1 (AA 22-35) |
| Positive Control | WB: HeLa, MCF7, Hep G2, Raji, A431, HEK293, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human RCC1 Antibody, Recombinant, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant RCC1 Antibody (Ab124863) - Western Blot
All lanes: Recombinant RCC1 Antibody (Ab124863) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 47 kDa
Exposure time: 10.0 s
Recombinant RCC1 Antibody (Ab124863) - Western Blot
All lanes: Recombinant RCC1 Antibody (Ab124863) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 47 kDa
Exposure time: 20.0 s
Recombinant RCC1 Antibody (Ab124863) - IF/ICC
Immunocytochemistry analysis of RCC1 (green) in HeLa using Recombinant RCC1 Antibody (Ab124863), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404032 | Certificate of Analysis | Apr 01, 2024 | Ab124863 |
| ZJ24F0404031 | Certificate of Analysis | Apr 01, 2024 | Ab124863 |
| 1. Furuno, N N and 6 more authors.. (1991) Complete nucleotide sequence of the human RCC1 gene involved in coupling between DNA replication and mitosis.. Genomics, [PMID:1769659] |
| 2. Bischoff, F R FR and Ponstingl, H H.. (1991) Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1.. Nature, (7): [PMID:1944575] |
| 3. Bischoff, F R FR, Maier, G G, Tilz, G G and Ponstingl, H H.. (1990) A 47-kDa human nuclear protein recognized by antikinetochore autoimmune sera is homologous with the protein encoded by RCC1, a gene implicated in onset of chromosome condensation.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2236072] |
| 4. Ohtsubo, M M and 9 more authors.. (1987) Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation.. Genes & development, [PMID:3678831] |
| 5. Miyabashira, J J, Sekiguchi, T T and Nishimoto, T T.. (1994) Mammalian cells have two functional RCC1 proteins produced by alternative splicing.. Journal of cell science, [PMID:7983178] |
| 6. Renault, L L and 6 more authors.. (1998) The 1.7 A crystal structure of the regulator of chromosome condensation (RCC1) reveals a seven-bladed propeller.. Nature, (5): [PMID:9510255] |
| 7. Renault, L L, Kuhlmann, J J, Henkel, A A and Wittinghofer, A A.. (2001) Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1).. Cell, (20): [PMID:11336674] |
| 8. Moore, William W, Zhang, Chuanmao C and Clarke, Paul R PR.. (2002) Targeting of RCC1 to chromosomes is required for proper mitotic spindle assembly in human cells.. Current biology : CB, (20): [PMID:12194828] |
| 9. Neuhaus, Eva M EM, Mashukova, Anastasia A, Barbour, Jon J, Wolters, Dirk D and Hatt, Hanns H.. (2006) Novel function of beta-arrestin2 in the nucleus of mature spermatozoa.. Journal of cell science, (1): [PMID:16820410] |
| 10. Chen, Ting T and 5 more authors.. (2007) N-terminal alpha-methylation of RCC1 is necessary for stable chromatin association and normal mitosis.. Nature cell biology, [PMID:17435751] |
| 11. Hao, Yi Y and Macara, Ian G IG.. (2008) Regulation of chromatin binding by a conformational switch in the tail of the Ran exchange factor RCC1.. The Journal of cell biology, (8): [PMID:18762580] |
| 12. Tooley, Christine E Schaner CE and 8 more authors.. (2010) NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein.. Nature, (26): [PMID:20668449] |
| 13. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 14. Halpin, David D, Kalab, Petr P, Wang, Jay J, Weis, Karsten K and Heald, Rebecca R.. (2011) Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.. PLoS biology, [PMID:22215983] |
| 15. Bao, Xiaoling X and 19 more authors.. (2018) Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation.. Journal of molecular cell biology, (1): [PMID:29040603] |
| 16. Sankhala, Rajeshwer S RS and 5 more authors.. (2017) Three-dimensional context rather than NLS amino acid sequence determines importin α subtype specificity for RCC1.. Nature communications, (17): [PMID:29042532] |
