Call +1 (833) 552-7181 or Contact us Earn $25 in coupons on your first order with Aladdin!
Toggle Nav
My Cart
Search
Advanced Search
Menu
Sign In
྾
main product photo

Recombinant RIG-I/DDX58 Antibody - Primary antibody, specific to RIGI, Rabbit IgG

    Application:
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab125359
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab125359-10μl
10μl
In stock
$66.90
Ab125359-50μl
50μl
In stock
$189.90
Ab125359-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab125359-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90
Add to Wish List Compare
Bulk Order  DATASHEET

Recombinant; Rabbit anti Human RIG-I Antibody; WB; Unconjugated

View related series
Click to view target information: DDX58
Accession#:O95786 DDX58 Gene ID:23586 RIGI

Basic Description

Product NameRecombinant RIG-I/DDX58 Antibody - Primary antibody, specific to RIGI, Rabbit IgG
SynonymsDdx58 antibody | DDX58_HUMAN antibody | DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody | DEAD (Asp Glu Ala Asp/His) box polypeptideantibody | DEAD box protein 58 antibody | DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 antibody | DKFZp434J1111 ant
Specifications & PurityExactAb™, Validated, Recombinant, 3.0 mg/mL
Host speciesRabbit
SpecificityRIGI
ImmunogenA synthetic peptide derived from human DDX58 (AA 220-450)
Positive ControlWB: Jurkat, A549, HeLa and HEK293 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human RIG-I Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function
Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration3.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant RIG-I/DDX58 Antibody (Ab125359) - Western Blot
All lanes: Recombinant RIG-I/DDX58 Antibody (Ab125359) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 107 kDa
Observed band size: 112 kDa
Exposure time: 50.0 s

Associated Targets(Human)

DDX58 Tbio Probable ATP-dependent RNA helicase DDX58 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/1000

">

1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0202936Certificate of AnalysisMar 01, 2024 Ab125359
ZJ24F0202937Certificate of AnalysisMar 01, 2024 Ab125359

Related Documents

 SDS

 Specification Sheet

FAQs and Articles

FAQ
Protocol of Western Blotting For High Molecular Weights
Categories: Protocols
Tags:
Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861] [10.1021/op500134e]
2. Matsumiya T, Stafforini DM.  (2010)  Function and regulation of retinoic acid-inducible gene-I..  Crit Rev Immunol,  30  (6): (489-513).  [PMID:21175414] [10.1021/op500134e]
3. Cui J, Zhu L, Xia X, Wang HY, Legras X, Hong J, Ji J, Shen P, Zheng S, Chen ZJ et al..  (2010)  NLRC5 negatively regulates the NF-kappaB and type I interferon signaling pathways..  Cell,  141  (3): (483-96).  [PMID:20434986] [10.1021/op500134e]
4. Imaizumi, Tadaatsu T and 12 more authors..  (2002)  Retinoic acid-inducible gene-I is induced in endothelial cells by LPS and regulates expression of COX-2..  Biochemical and biophysical research communications,    (22):   [PMID:11890704]
5. Humphray, S J SJ and 147 more authors..  (2004)  DNA sequence and analysis of human chromosome 9..  Nature,    (27):   [PMID:15164053]
6. Cui, Xue-Fan XF, Imaizumi, Tadaatsu T, Yoshida, Hidemi H, Borden, Ernest C EC and Satoh, Kei K..  (2004)  Retinoic acid-inducible gene-I is induced by interferon-gamma and regulates the expression of interferon-gamma stimulated gene 15 in MCF-7 cells..  Biochemistry and cell biology = Biochimie et biologie cellulaire,      [PMID:15181474]
7. Yoneyama, Mitsutoshi M and 8 more authors..  (2004)  The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses..  Nature immunology,      [PMID:15208624]
8. Imaizumi, Tadaatsu T and 10 more authors..  (2004)  Expression of retinoic acid-inducible gene-I in vascular smooth muscle cells stimulated with interferon-gamma..  Life sciences,    (23):   [PMID:15219805]
9. Sumpter, Rhea R and 7 more authors..  (2005)  Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I..  Journal of virology,      [PMID:15708988]
10. Zhao, Chen C, Denison, Carilee C, Huibregtse, Jon M JM, Gygi, Steven S and Krug, Robert M RM..  (2005)  Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways..  Proceedings of the National Academy of Sciences of the United States of America,    (19):   [PMID:16009940]
11. Seth, Rashu B RB, Sun, Lijun L, Ea, Chee-Kwee CK and Chen, Zhijian J ZJ..  (2005)  Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3..  Cell,    (9):   [PMID:16125763]
12. Kawai, Taro T and 8 more authors..  (2005)  IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction..  Nature immunology,      [PMID:16127453]
13. Xu, Liang-Guo LG and 5 more authors..  (2005)  VISA is an adapter protein required for virus-triggered IFN-beta signaling..  Molecular cell,    (16):   [PMID:16153868]
14. Huang, Jun J and 5 more authors..  (2005)  SIKE is an IKK epsilon/TBK1-associated suppressor of TLR3- and virus-triggered IRF-3 activation pathways..  The EMBO journal,    (7):   [PMID:16281057]
15. Saito, Takeshi T and 8 more authors..  (2007)  Regulation of innate antiviral defenses through a shared repressor domain in RIG-I and LGP2..  Proceedings of the National Academy of Sciences of the United States of America,    (9):   [PMID:17190814]
16. Gack, Michaela U MU and 10 more authors..  (2007)  TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity..  Nature,    (19):   [PMID:17392790]
17. Arimoto, Kei-ichiro K and 5 more authors..  (2007)  Negative regulation of the RIG-I signaling by the ubiquitin ligase RNF125..  Proceedings of the National Academy of Sciences of the United States of America,    (1):   [PMID:17460044]
18. Kim, Min-Jung MJ, Hwang, Sun-Young SY, Imaizumi, Tadaatsu T and Yoo, Joo-Yeon JY..  (2008)  Negative feedback regulation of RIG-I-mediated antiviral signaling by interferon-induced ISG15 conjugation..  Journal of virology,      [PMID:18057259]
19. Takahasi, Kiyohiro K and 8 more authors..  (2008)  Nonself RNA-sensing mechanism of RIG-I helicase and activation of antiviral immune responses..  Molecular cell,    (29):   [PMID:18242112]
20. Cui, Sheng S and 9 more authors..  (2008)  The C-terminal regulatory domain is the RNA 5'-triphosphate sensor of RIG-I..  Molecular cell,    (1):   [PMID:18243112]
21. Friedman, Constantin S CS and 11 more authors..  (2008)  The tumour suppressor CYLD is a negative regulator of RIG-I-mediated antiviral response..  EMBO reports,      [PMID:18636086]
22. Ishikawa, Hiroki H and Barber, Glen N GN..  (2008)  STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling..  Nature,    (2):   [PMID:18724357]
23. Oshiumi, Hiroyuki H, Matsumoto, Misako M, Hatakeyama, Shigetsugu S and Seya, Tsukasa T..  (2009)  Riplet/RNF135, a RING finger protein, ubiquitinates RIG-I to promote interferon-beta induction during the early phase of viral infection..  The Journal of biological chemistry,    (9):   [PMID:19017631]
24. Mukherjee, Amitava A and 7 more authors..  (2009)  Retinoic acid-induced gene-1 (RIG-I) associates with the actin cytoskeleton via caspase activation and recruitment domain-dependent interactions..  The Journal of biological chemistry,    (6):   [PMID:19122199]
25. Ling, Zhenhua Z, Tran, Kim C KC and Teng, Michael N MN..  (2009)  Human respiratory syncytial virus nonstructural protein NS2 antagonizes the activation of beta interferon transcription by interacting with RIG-I..  Journal of virology,      [PMID:19193793]
26. Bamming, Darja D and Horvath, Curt M CM..  (2009)  Regulation of signal transduction by enzymatically inactive antiviral RNA helicase proteins MDA5, RIG-I, and LGP2..  The Journal of biological chemistry,    (10):   [PMID:19211564]
27. Johnsen, Ingvild B IB, Nguyen, Thuy Thanh TT, Bergstroem, Bjarte B, Fitzgerald, Katherine A KA and Anthonsen, Marit W MW..  (2009)  The tyrosine kinase c-Src enhances RIG-I (retinoic acid-inducible gene I)-elicited antiviral signaling..  The Journal of biological chemistry,    (10):   [PMID:19419966]
28. Gao, Dong D and 8 more authors..  (2009)  REUL is a novel E3 ubiquitin ligase and stimulator of retinoic-acid-inducible gene-I..  PloS one,    (1):   [PMID:19484123]
29. Schlee, Martin M and 20 more authors..  (2009)  Recognition of 5' triphosphate by RIG-I helicase requires short blunt double-stranded RNA as contained in panhandle of negative-strand virus..  Immunity,    (17):   [PMID:19576794]
30. Ablasser, Andrea A and 5 more authors..  (2009)  RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate..  Nature immunology,      [PMID:19609254]
31. Chiu, Yu-Hsin YH, Macmillan, John B JB and Chen, Zhijian J ZJ..  (2009)  RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway..  Cell,    (7):   [PMID:19631370]
32. Fan, Lina L, Briese, Thomas T and Lipkin, W Ian WI..  (2010)  Z proteins of New World arenaviruses bind RIG-I and interfere with type I interferon induction..  Journal of virology,      [PMID:20007272]
33. Oshiumi, Hiroyuki H, Sakai, Keisuke K, Matsumoto, Misako M and Seya, Tsukasa T..  (2010)  DEAD/H BOX 3 (DDX3) helicase binds the RIG-I adaptor IPS-1 to up-regulate IFN-beta-inducing potential..  European journal of immunology,      [PMID:20127681]
34. Chen, Rui R and 5 more authors..  (2010)  The ubiquitin-specific protease 17 is involved in virus-triggered type I IFN signaling..  Cell research,      [PMID:20368735]
35. Onoguchi, Kazuhide K, Yoneyama, Mitsutoshi M and Fujita, Takashi T..  (2011)  Retinoic acid-inducible gene-I-like receptors..  Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research,      [PMID:20950133]
36. Sun, Zhiguo Z, Ren, Hongwei H, Liu, Yan Y, Teeling, Jessica L JL and Gu, Jun J..  (2011)  Phosphorylation of RIG-I by casein kinase II inhibits its antiviral response..  Journal of virology,      [PMID:21068236]
37. Hayakawa, Sumio S and 18 more authors..  (2011)  ZAPS is a potent stimulator of signaling mediated by the RNA helicase RIG-I during antiviral responses..  Nature immunology,      [PMID:21102435]
38. Yang, Yong-Kang YK and 7 more authors..  (2011)  ARF-like protein 16 (ARL16) inhibits RIG-I by binding with its C-terminal domain in a GTP-dependent manner..  The Journal of biological chemistry,    (25):   [PMID:21233210]
39. Loo, Yueh-Ming YM and Gale, Michael M..  (2011)  Immune signaling by RIG-I-like receptors..  Immunity,    (27):   [PMID:21616437]
40. Miyashita, Moeko M, Oshiumi, Hiroyuki H, Matsumoto, Misako M and Seya, Tsukasa T..  (2011)  DDX60, a DEXD/H box helicase, is a novel antiviral factor promoting RIG-I-like receptor-mediated signaling..  Molecular and cellular biology,      [PMID:21791617]
41. Liu, Xin-Yi XY, Chen, Wei W, Wei, Bo B, Shan, Yu-Fei YF and Wang, Chen C..  (2011)  IFN-induced TPR protein IFIT3 potentiates antiviral signaling by bridging MAVS and TBK1..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:21813773]
42. Qin, Lan L and 8 more authors..  (2011)  Rotavirus nonstructural protein 1 antagonizes innate immune response by interacting with retinoic acid inducible gene I..  Virology journal,    (8):   [PMID:22152002]
43. Xing, Junji J, Wang, Shuai S, Lin, Rongtuan R, Mossman, Karen L KL and Zheng, Chunfu C..  (2012)  Herpes simplex virus 1 tegument protein US11 downmodulates the RLR signaling pathway via direct interaction with RIG-I and MDA-5..  Journal of virology,      [PMID:22301138]
44. Jenkins, Kristie K and 11 more authors..  (2013)  Mitochondrially localised MUL1 is a novel modulator of antiviral signaling..  Immunology and cell biology,      [PMID:23399697]
45. Li, Meng-Tong MT and 7 more authors..  (2013)  Negative regulation of RIG-I-mediated innate antiviral signaling by SEC14L1..  Journal of virology,      [PMID:23843640]
46. Oshiumi, Hiroyuki H, Miyashita, Moeko M, Matsumoto, Misako M and Seya, Tsukasa T..  (2013)  A distinct role of Riplet-mediated K63-Linked polyubiquitination of the RIG-I repressor domain in human antiviral innate immune responses..  PLoS pathogens,      [PMID:23950712]
47. Feng, Qian Q and 10 more authors..  (2014)  Enterovirus 2Apro targets MDA5 and MAVS in infected cells..  Journal of virology,      [PMID:24390337]
48. Santiago, Felix W FW and 9 more authors..  (2014)  Hijacking of RIG-I signaling proteins into virus-induced cytoplasmic structures correlates with the inhibition of type I interferon responses..  Journal of virology,      [PMID:24478431]
49. Yan, Jie J, Li, Qi Q, Mao, Ai-Ping AP, Hu, Ming-Ming MM and Shu, Hong-Bing HB..  (2014)  TRIM4 modulates type I interferon induction and cellular antiviral response by targeting RIG-I for K63-linked ubiquitination..  Journal of molecular cell biology,      [PMID:24755855]
50. Jang, Mi-Ae MA and 25 more authors..  (2015)  Mutations in DDX58, which encodes RIG-I, cause atypical Singleton-Merten syndrome..  American journal of human genetics,    (5):   [PMID:25620203]
51. Hao, Qian Q and 12 more authors..  (2015)  A non-canonical role of the p97 complex in RIG-I antiviral signaling..  The EMBO journal,    (2):   [PMID:26471729]
52. Wang, Shuai S and 7 more authors..  (2016)  RNF123 has an E3 ligase-independent function in RIG-I-like receptor-mediated antiviral signaling..  EMBO reports,      [PMID:27312109]
53. Wang, Peng P and 5 more authors..  (2016)  The nucleolar protein GLTSCR2 is required for efficient viral replication..  Scientific reports,    (8):   [PMID:27824081]
54. Zhao, Jun and 12 more authors..  (2016)  A Viral Deamidase Targets the Helicase Domain of RIG-I to Block RNA-Induced Activation..  Cell host & microbe,    (14):   [PMID:27866900]
55. Shi, Yuheng Y and 7 more authors..  (2017)  Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity..  Nature communications,    (4):   [PMID:28469175]
56. Du, Yang Y and 6 more authors..  (2018)  LRRC25 inhibits type I IFN signaling by targeting ISG15-associated RIG-I for autophagic degradation..  The EMBO journal,    (1):   [PMID:29288164]
57. Lian, Huan H and 12 more authors..  (2018)  The Zinc-Finger Protein ZCCHC3 Binds RNA and Facilitates Viral RNA Sensing and Activation of the RIG-I-like Receptors..  Immunity,    (18):   [PMID:30193849]
58. Tao, Xinyue; Chu, Bei; Xin, Di; Li, Lin and Sun, Qinmiao..  (2020)  USP27X negatively regulates antiviral signaling by deubiquitinating RIG-I..  PLoS pathogens,      [PMID:32027733]
59. Jena, Kautilya Kumar and 16 more authors..  (2020)  Autoimmunity gene IRGM suppresses cGAS-STING and RIG-I-MAVS signaling to control interferon response..  EMBO reports,    (3):   [PMID:32715615]

Solution Calculators

Privacy Policy Website Terms of Use Return policy Terms and Conditions of Sale Cookie Policy Sales Policy
Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.
Copyright © 2023-present Aladdin Scientific Corp . All rights reserved.