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Ab125359-10μl

10μl

In stock

$66.90

Ab125359-50μl

50μl

In stock

$189.90

Ab125359-100μl

100μl

Available within 8-12 weeks(?)

$299.90

Ab125359-1ml

1ml

Available within 8-12 weeks(?)

$2,699.90

Basic Description

Product Name	Recombinant RIG-I/DDX58 Antibody - Primary antibody, specific to RIGI, Rabbit IgG
Synonyms	Ddx58 antibody; DDX58_HUMAN antibody; DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody; DEAD (Asp Glu Ala Asp/His) box polypeptideantibody; DEAD box protein 58 antibody; DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 antibody; DKFZp434J1111 antibody;
Specifications & Purity	ExactAb™, Validated, Recombinant, 3.0 mg/mL
Host species	Rabbit
Specificity	RIGI
Immunogen	A synthetic peptide derived from human DDX58 (AA 220-450)
Positive Control	WB: Jurkat, A549, HeLa and HEK293 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human RIG-I Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	3.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant RIG-I/DDX58 Antibody (Ab125359) - Western Blot
All lanes: Recombinant RIG-I/DDX58 Antibody (Ab125359) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 107 kDa
Observed band size: 112 kDa
Exposure time: 50.0 s

Associated Targets(Human)

DDX58 Tbio Probable ATP-dependent RNA helicase DDX58 (0 Activities)

Discover Target: DDX58

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0202936	Certificate of Analysis	Mar 01, 2024	Ab125359
ZJ24F0202937	Certificate of Analysis	Mar 01, 2024	Ab125359

FAQs and Articles

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Protocol of Western Blotting For High Molecular Weights

Categories: Protocols

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

References

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Recombinant RIG-I/DDX58 Antibody - Primary antibody, specific to RIGI, Rabbit IgG