Recombinant; Rabbit anti Human RING1 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab125377-10μl | 10μl | In stock | $69.90 | |
| Ab125377-50μl | 50μl | In stock | $189.90 | |
| Ab125377-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab125377-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human RING1 Antibody; WB; Unconjugated
| Product Name | Recombinant RING1 Antibody - Primary antibody, specific to RING1, Rabbit IgG |
|---|---|
| Synonyms | Ring1A antibody | E3 ubiquitin-protein ligase RING1 antibody | Polycomb complex protein RING1 antibody | Really interesting new gene 1 protein antibody | RING finger protein 1 antibody | Ring1 antibody | RING1_HUMAN antibody | Rnf1 antibody | Transcriptio |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.4 mg/mL |
| Host species | Rabbit |
| Specificity | RING1 |
| Immunogen | A synthetic peptide derived from human RING1 (AA 140-195) |
| Positive Control | WB: Ramos, HeLa, LNCaP, MOLT-4, THP-1 and NIH/3T3 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human RING1 Antibody, Recombinant, could be used for WB and so on. Application: |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant RING1 Antibody (Ab125377) - Western Blot
All lanes: Recombinant RING1 Antibody (Ab125377) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 49, 50 kDa
Exposure time: 14.5 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0405041 | Certificate of Analysis | Apr 29, 2024 | Ab125377 |
| ZJ24F0405040 | Certificate of Analysis | Apr 29, 2024 | Ab125377 |
| 1. Lovering, R R and 9 more authors.. (1993) Identification and preliminary characterization of a protein motif related to the zinc finger.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:7681583] |
| 2. Satijn, D P DP and 9 more authors.. (1997) RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor.. Molecular and cellular biology, [PMID:9199346] |
| 3. Satijn, D P DP and Otte, A P AP.. (1999) RING1 interacts with multiple Polycomb-group proteins and displays tumorigenic activity.. Molecular and cellular biology, [PMID:9858531] |
| 4. Ogawa, Hidesato H, Ishiguro, Kei-Ichiro K, Gaubatz, Stefan S, Livingston, David M DM and Nakatani, Yoshihiro Y.. (2002) A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells.. Science (New York, N.Y.), (10): [PMID:12004135] |
| 5. Akasaka, Takeshi T and 5 more authors.. (2002) MBLR, a new RING finger protein resembling mammalian Polycomb gene products, is regulated by cell cycle-dependent phosphorylation.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:12167161] |
| 6. Levine, Stuart S SS and 5 more authors.. (2002) The core of the polycomb repressive complex is compositionally and functionally conserved in flies and humans.. Molecular and cellular biology, [PMID:12167701] |
| 7. Wang, Hengbin H and 6 more authors.. (2004) Role of histone H2A ubiquitination in Polycomb silencing.. Nature, (14): [PMID:15386022] |
| 8. Cao, Ru R, Tsukada, Yu-Ichi Y and Zhang, Yi Y.. (2005) Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing.. Molecular cell, (22): [PMID:16359901] |
| 9. Gearhart, Micah D MD, Corcoran, Connie M CM, Wamstad, Joseph A JA and Bardwell, Vivian J VJ.. (2006) Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets.. Molecular and cellular biology, [PMID:16943429] |
| 10. Maertens, Goedele N GN and 7 more authors.. (2009) Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus.. PloS one, (28): [PMID:19636380] |
| 11. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 12. Vandamme, Julien J, Völkel, Pamela P, Rosnoblet, Claire C, Le Faou, Perrine P and Angrand, Pierre-Olivier PO.. (2011) Interaction proteomics analysis of polycomb proteins defines distinct PRC1 complexes in mammalian cells.. Molecular & cellular proteomics : MCP, [PMID:21282530] |
| 13. Blanchard, Maxime G MG and 13 more authors.. (2015) De novo gain-of-function and loss-of-function mutations of SCN8A in patients with intellectual disabilities and epilepsy.. Journal of medical genetics, [PMID:25725044] |
| 14. Miyake, Noriko and 22 more authors.. (2020) Gain-of-Function MN1 Truncation Variants Cause a Recognizable Syndrome with Craniofacial and Brain Abnormalities.. American journal of human genetics, (2): [PMID:31839203] |
