Recombinant; Rabbit anti Human RING2/RING1B/RNF2 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab125386-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab125386-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab125386-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $319.90 | |
| Ab125386-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human RING2/RING1B/RNF2 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant RING2/RING1B/RNF2 Antibody - Primary antibody, specific to RING2/RING1B/RNF2, Rabbit IgG |
|---|---|
| Synonyms | EC 2.3.2.27 | HIP2-interacting protein 3 | Huntingtin-interacting protein 2-interacting protein 3 | Protein DinG | RING finger protein 1B | RING finger protein 2 | RING finger protein BAP-1 | RING1b | RING-type E3 ubiquitin transferase RING2 | BAP 1 antib |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.8 mg/mL |
| Host species | Rabbit |
| Specificity | RING2/RING1B/RNF2 |
| Immunogen | A synthetic peptide derived from human RING2 / RING1B / RNF2 (AA 118-131) |
| Positive Control | WB: HeLa, A549, U-2 OS, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human RING2/RING1B/RNF2 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) - Western Blot
All lanes: Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 38 kDa
Observed band size: 41 kDa
Exposure time: 137.7 s
Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) - Western Blot
All lanes: Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 38 kDa
Observed band size: 41 kDa
Exposure time: 60.0 s
Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant RING2/RING1B/RNF2 Antibody (Ab125386). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0607279 | Certificate of Analysis | Jun 24, 2024 | Ab125386 |
| ZJ24F0607278 | Certificate of Analysis | Jun 24, 2024 | Ab125386 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Lee, S J SJ and 5 more authors.. (2001) E3 ligase activity of RING finger proteins that interact with Hip-2, a human ubiquitin-conjugating enzyme.. FEBS letters, (10): [PMID:11513855] |
| 3. Tuckfield, Annabel A and 5 more authors.. (2002) Binding of the RING polycomb proteins to specific target genes in complex with the grainyhead-like family of developmental transcription factors.. Molecular and cellular biology, [PMID:11865070] |
| 4. Ogawa, Hidesato H, Ishiguro, Kei-Ichiro K, Gaubatz, Stefan S, Livingston, David M DM and Nakatani, Yoshihiro Y.. (2002) A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells.. Science (New York, N.Y.), (10): [PMID:12004135] |
| 5. Levine, Stuart S SS and 5 more authors.. (2002) The core of the polycomb repressive complex is compositionally and functionally conserved in flies and humans.. Molecular and cellular biology, [PMID:12167701] |
| 6. Wang, Hengbin H and 6 more authors.. (2004) Role of histone H2A ubiquitination in Polycomb silencing.. Nature, (14): [PMID:15386022] |
| 7. Dou, Yali Y and 9 more authors.. (2005) Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF.. Cell, (17): [PMID:15960975] |
| 8. Cao, Ru R, Tsukada, Yu-Ichi Y and Zhang, Yi Y.. (2005) Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing.. Molecular cell, (22): [PMID:16359901] |
| 9. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 10. Li, Zhizhong Z and 5 more authors.. (2006) Structure of a Bmi-1-Ring1B polycomb group ubiquitin ligase complex.. The Journal of biological chemistry, (21): [PMID:16714294] |
| 11. Gearhart, Micah D MD, Corcoran, Connie M CM, Wamstad, Joseph A JA and Bardwell, Vivian J VJ.. (2006) Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets.. Molecular and cellular biology, [PMID:16943429] |
| 12. Maertens, Goedele N GN and 7 more authors.. (2009) Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus.. PloS one, (28): [PMID:19636380] |
| 13. Wang, Renjing R and 12 more authors.. (2010) Polycomb group targeting through different binding partners of RING1B C-terminal domain.. Structure (London, England : 1993), (11): [PMID:20696397] |
| 14. Vandamme, Julien J, Völkel, Pamela P, Rosnoblet, Claire C, Le Faou, Perrine P and Angrand, Pierre-Olivier PO.. (2011) Interaction proteomics analysis of polycomb proteins defines distinct PRC1 complexes in mammalian cells.. Molecular & cellular proteomics : MCP, [PMID:21282530] |
| 15. Bentley, Matthew L ML and 5 more authors.. (2011) Recognition of UbcH5c and the nucleosome by the Bmi1/Ring1b ubiquitin ligase complex.. The EMBO journal, (19): [PMID:21772249] |
| 16. McGinty, Robert K; Henrici, Ryan C and Tan, Song.. (2014) Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome.. Nature, (30): [PMID:25355358] |
| 17. Gao, Zhonghua Z and 5 more authors.. (2014) An AUTS2-Polycomb complex activates gene expression in the CNS.. Nature, (18): [PMID:25519132] |
| 18. Taherbhoy, Asad M AM, Huang, Oscar W OW and Cochran, Andrea G AG.. (2015) BMI1-RING1B is an autoinhibited RING E3 ubiquitin ligase.. Nature communications, (7): [PMID:26151332] |
| 19. Oliviero, Giorgio G and 7 more authors.. (2015) The variant Polycomb Repressor Complex 1 component PCGF1 interacts with a pluripotency sub-network that includes DPPA4, a regulator of embryogenesis.. Scientific reports, (21): [PMID:26687479] |
| 20. Santofimia-Castaño, Patricia P and 7 more authors.. (2017) Intrinsically disordered chromatin protein NUPR1 binds to the C-terminal region of Polycomb RING1B.. Proceedings of the National Academy of Sciences of the United States of America, (18): [PMID:28720707] |
