Recombinant; Rabbit anti Human RPS3 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab125945-10μl | 10μl | In stock | $69.90 | |
| Ab125945-50μl | 50μl | In stock | $199.90 | |
| Ab125945-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $319.90 | |
| Ab125945-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human RPS3 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant RPS3 Antibody - Primary antibody, specific to RPS3, Rabbit IgG |
|---|---|
| Synonyms | 40S ribosomal protein S3 antibody | fb13d09 antibody | FLJ26283 antibody | FLJ27450 antibody | IMR 90 ribosomal protein S3 antibody | MGC56088 antibody | MGC87870 antibody | OTTHUMP00000229804 antibody | OTTHUMP00000229805 antibody | OTTHUMP00000229874 an |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.38 mg/mL |
| Host species | Rabbit |
| Specificity | RPS3 |
| Immunogen | A synthetic peptide derived from human RPS3 (AA 114-184) |
| Positive Control | WB: Hep G2, HeLa, NIH/3T3 and C6 cell lysates. IHC: Human colon cancer tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human RPS3 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.38 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant RPS3 Antibody (Ab125945) - Western Blot
All lanes: Recombinant RPS3 Antibody (Ab125945) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 33 kDa
Exposure time: 49.5 s
Recombinant RPS3 Antibody (Ab125945) - Western Blot
All lanes: Recombinant RPS3 Antibody (Ab125945) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 33 kDa
Exposure time: 23.1 s
Recombinant RPS3 Antibody (Ab125945) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant RPS3 Antibody (Ab125945). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505353 | Certificate of Analysis | May 10, 2024 | Ab125945 |
| ZJ24F0505352 | Certificate of Analysis | May 10, 2024 | Ab125945 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] |
| 3. Xu S, Luo S, Zhu Z, Xu J. (2019) Small molecules as inhibitors of PCSK9: Current status and future challenges.. Eur J Med Chem, 162 (3): (212-233). [PMID:30448414] |
| 4. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 5. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211] |
| 6. Elayanambi Sundaramoorthy,Marilyn Leonard,Raymond Mak,Jeffrey Liao,Amitkumar Fulzele,Eric J Bennett. (2017-01-31) ZNF598 and RACK1 Regulate Mammalian Ribosome-Associated Quality Control Function by Mediating Regulatory 40S Ribosomal Ubiquitylation.. Molecular cell, 65 ((4)): (751-760). [PMID:28132843] |
| 7. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 8. Kandasamy J, Atia-Glikin D, Shulman E, Shapira K, Shavit M, Belakhov V, Baasov T.. (2012) Increased selectivity toward cytoplasmic versus mitochondrial ribosome confers improved efficiency of synthetic aminoglycosides in fixing damaged genes: a strategy for treatment of genetic diseases caused by nonsense mutations.. J Med Chem, 55 (23): (10630-10643). [PMID:23148581] |
| 9. Pogue-Geile, K K and 6 more authors.. (1991) Ribosomal protein genes are overexpressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ribosomal protein.. Molecular and cellular biology, [PMID:1712897] |
| 10. Zhang, X T XT, Tan, Y M YM and Tan, Y H YH.. (1990) Isolation of a cDNA encoding human 40S ribosomal protein s3.. Nucleic acids research, (25): [PMID:2129557] |
| 11. Kim, J J and 5 more authors.. (1995) Implication of mammalian ribosomal protein S3 in the processing of DNA damage.. The Journal of biological chemistry, (9): [PMID:7775413] |
| 12. Tycowski, K T KT, Shu, M D MD and Steitz, J A JA.. (1993) A small nucleolar RNA is processed from an intron of the human gene encoding ribosomal protein S3.. Genes & development, [PMID:8319909] |
| 13. Vladimirov, S N SN and 7 more authors.. (1996) Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry.. European journal of biochemistry, (1): [PMID:8706699] |
| 14. Yoshihama, Maki M and 10 more authors.. (2002) The human ribosomal protein genes: sequencing and comparative analysis of 73 genes.. Genome research, [PMID:11875025] |
| 15. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 16. Hegde, Vijay V, Wang, Mu M and Deutsch, Walter A WA.. (2004) Characterization of human ribosomal protein S3 binding to 7,8-dihydro-8-oxoguanine and abasic sites by surface plasmon resonance.. DNA repair, (3): [PMID:14706345] |
| 17. Jang, Chang-Young CY, Lee, Jae Yung JY and Kim, Joon J.. (2004) RpS3, a DNA repair endonuclease and ribosomal protein, is involved in apoptosis.. FEBS letters, (27): [PMID:14988002] |
| 18. Hegde, Vijay V, Wang, Mu M and Deutsch, Walter A WA.. (2004) Human ribosomal protein S3 interacts with DNA base excision repair proteins hAPE/Ref-1 and hOGG1.. Biochemistry, (9): [PMID:15518571] |
| 19. Kim, Sang Hwa SH, Lee, Jae Yung JY and Kim, Joon J.. (2005) Characterization of a wide range base-damage-endonuclease activity of mammalian rpS3.. Biochemical and biophysical research communications, (25): [PMID:15707971] |
| 20. Kim, Hag Dong HD, Lee, Jae Yung JY and Kim, Joon J.. (2005) Erk phosphorylates threonine 42 residue of ribosomal protein S3.. Biochemical and biophysical research communications, (22): [PMID:15950189] |
| 21. Kim, Tae-Sung TS and 5 more authors.. (2006) Interaction of Hsp90 with ribosomal proteins protects from ubiquitination and proteasome-dependent degradation.. Molecular biology of the cell, [PMID:16314389] |
| 22. Hegde, Vijay V, Wang, Mu M, Mian, I Saira IS, Spyres, Lea L and Deutsch, Walter A WA.. (2006) The high binding affinity of human ribosomal protein S3 to 7,8-dihydro-8-oxoguanine is abrogated by a single amino acid change.. DNA repair, (13): [PMID:16737853] |
| 23. Beranova-Giorgianni, Sarka S, Zhao, Yingxin Y, Desiderio, Dominic M DM and Giorgianni, Francesco F.. (2006) Phosphoproteomic analysis of the human pituitary.. Pituitary, [PMID:16807684] |
| 24. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 25. Hegde, Vijay V, Yadavilli, Sridevi S and Deutsch, Walter A WA.. (2007) Knockdown of ribosomal protein S3 protects human cells from genotoxic stress.. DNA repair, (4): [PMID:17049931] |
| 26. Jønson, Lars L and 8 more authors.. (2007) Molecular composition of IMP1 ribonucleoprotein granules.. Molecular & cellular proteomics : MCP, [PMID:17289661] |
| 27. Yadavilli, Sridevi S, Hegde, Vijay V and Deutsch, Walter A WA.. (2007) Translocation of human ribosomal protein S3 to sites of DNA damage is dependant on ERK-mediated phosphorylation following genotoxic stress.. DNA repair, (1): [PMID:17560175] |
| 28. Wan, Fengyi F and 11 more authors.. (2007) Ribosomal protein S3: a KH domain subunit in NF-kappaB complexes that mediates selective gene regulation.. Cell, (30): [PMID:18045535] |
| 29. Balyeva, K E KE, Malygin, A A AA, Karpova, G G GG, Nevinskiĭ, G A GA and Zharkov, D O DO.. () [Interactions of human ribosomal protein S3 with undamaged and damaged DNA].. Molekuliarnaia biologiia, [PMID:18610840] |
| 30. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 31. Ko, Sung Il SI and 5 more authors.. (2008) Human ribosomal protein S3 (hRpS3) interacts with uracil-DNA glycosylase (hUNG) and stimulates its glycosylase activity.. Mutation research, (15): [PMID:18973764] |
| 32. Kim, Tae-Sung TS, Kim, Hag Dong HD and Kim, Joon J.. (2009) PKCdelta-dependent functional switch of rpS3 between translation and DNA repair.. Biochimica et biophysica acta, [PMID:19059439] |
| 33. Shin, Hyun-Seock HS and 5 more authors.. (2009) Arginine methylation of ribosomal protein S3 affects ribosome assembly.. Biochemical and biophysical research communications, (24): [PMID:19460357] |
| 34. Weinlich, Susan S and 5 more authors.. (2009) IGF2BP1 enhances HCV IRES-mediated translation initiation via the 3'UTR.. RNA (New York, N.Y.), [PMID:19541769] |
| 35. Yadavilli, Sridevi S and 5 more authors.. (2009) Ribosomal protein S3: A multi-functional protein that interacts with both p53 and MDM2 through its KH domain.. DNA repair, (2): [PMID:19656744] |
| 36. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 37. Gao, Xiaofei X and 11 more authors.. (2009) Bacterial effector binding to ribosomal protein s3 subverts NF-kappaB function.. PLoS pathogens, [PMID:20041225] |
| 38. Kim, Hag Dong HD and 7 more authors.. (2010) RpS3 translation is repressed by interaction with its own mRNA.. Journal of cellular biochemistry, (15): [PMID:20217897] |
| 39. Lee, Sang Bae SB and 9 more authors.. (2010) Ribosomal protein S3, a new substrate of Akt, serves as a signal mediator between neuronal apoptosis and DNA repair.. The Journal of biological chemistry, (17): [PMID:20605787] |
| 40. Wan, Fengyi F and 5 more authors.. (2011) IKKβ phosphorylation regulates RPS3 nuclear translocation and NF-κB function during infection with Escherichia coli strain O157:H7.. Nature immunology, [PMID:21399639] |
| 41. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 42. Yoon, In-Soo IS and 9 more authors.. (2011) Ribosomal protein S3 is phosphorylated by Cdk1/cdc2 during G2/M phase.. BMB reports, [PMID:21871177] |
| 43. Jang, Chang-Young CY and 5 more authors.. (2011) Ribosomal protein S3 is stabilized by sumoylation.. Biochemical and biophysical research communications, (28): [PMID:21968017] |
| 44. Jang, Chang-Young CY, Kim, Hag Dong HD and Kim, Joon J.. (2012) Ribosomal protein S3 interacts with TRADD to induce apoptosis through caspase dependent JNK activation.. Biochemical and biophysical research communications, (11): [PMID:22510408] |
| 45. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 46. Jang, Chang-Young CY, Kim, Hag Dong HD, Zhang, Xianghua X, Chang, Jin-Soo JS and Kim, Joon J.. (2012) Ribosomal protein S3 localizes on the mitotic spindle and functions as a microtubule associated protein in mitosis.. Biochemical and biophysical research communications, (7): [PMID:23131551] |
| 47. Anger, Andreas M AM and 6 more authors.. (2013) Structures of the human and Drosophila 80S ribosome.. Nature, (2): [PMID:23636399] |
| 48. Kim, YongJoong Y, Kim, Hag Dong HD and Kim, Joon J.. (2013) Cytoplasmic ribosomal protein S3 (rpS3) plays a pivotal role in mitochondrial DNA damage surveillance.. Biochimica et biophysica acta, [PMID:23911537] |
| 49. Lee, Kyung-Ha KH and 9 more authors.. (2014) AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation.. Nucleic acids research, [PMID:24423872] |
| 50. Stanborough, Tamsyn T, Niederhauser, Johannes J, Koch, Barbara B, Bergler, Helmut H and Pertschy, Brigitte B.. (2014) Ribosomal protein S3 interacts with the NF-κB inhibitor IκBα.. FEBS letters, (3): [PMID:24457201] |
| 51. Ban, Nenad N and 24 more authors.. (2014) A new system for naming ribosomal proteins.. Current opinion in structural biology, [PMID:24524803] |
| 52. Juszkiewicz, Szymon S and Hegde, Ramanujan S RS.. (2017) Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination.. Molecular cell, (16): [PMID:28065601] |
