| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab125976-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab125976-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab125976-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab125976-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human RPS6 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant RPS6 Antibody - Primary antibody, specific to RPS6, Rabbit IgG |
|---|---|
| Synonyms | Phosphoprotein NP33, 40S ribosomal protein S6 | pS6 | Ribosomal Protein S6 | RPS6 | S6 | 40S ribosomal protein S6 antibody | Air8 antibody | NP33 antibody | Phosphoprotein NP33 antibody | Pp30 antibody | Ribosomal protein S6 antibody | RP S6 antibody | rp |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | RPS6 |
| Immunogen | Recombinant protein of human S6 Ribosomal Protein (AA 1-249) |
| Positive Control | WB: Raji and RAW 264.7 cell lysates. IHC: Human breast cancer tissue. ICC/IF: HeLa cells. IP: HeLa lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human RPS6 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant RPS6 Antibody (Ab125976) - Western Blot
All lanes: Recombinant RPS6 Antibody (Ab125976) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 1.0 s
Recombinant RPS6 Antibody (Ab125976) - IF/ICC
Immunocytochemistry analysis of HeLa using Recombinant RPS6 Antibody (Ab125976).
Recombinant RPS6 Antibody (Ab125976) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant RPS6 Antibody (Ab125976). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant RPS6 Antibody (Ab125976) - IP
Immunoprecipitation analysis of HeLa cell lysates using Recombinant RPS6 Antibody (Ab125976).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] [10.1021/op500134e] |
| 3. Xu S, Luo S, Zhu Z, Xu J. (2019) Small molecules as inhibitors of PCSK9: Current status and future challenges.. Eur J Med Chem, 162 (13): (212-233). [PMID:30448414] [10.1021/op500134e] |
| 4. Nitin S Nandurkar,Jianjun Zhang,Qing Ye,Larissa V Ponomareva,Qing-Bai She,Jon S Thorson. (2014-08-15) The identification of perillyl alcohol glycosides with improved antiproliferative activity.. Journal of medicinal chemistry, 57 ((17)): (7478-7484). [PMID:25121720] |
| 5. Kandasamy J, Atia-Glikin D, Shulman E, Shapira K, Shavit M, Belakhov V, Baasov T.. (2012) Increased selectivity toward cytoplasmic versus mitochondrial ribosome confers improved efficiency of synthetic aminoglycosides in fixing damaged genes: a strategy for treatment of genetic diseases caused by nonsense mutations.. J Med Chem, 55 (23): (10630-10643). [PMID:23148581] [10.1021/jm3012992] |
| 6. Antoine, M M and Fried, M M.. (1992) The organization of the intron-containing human S6 ribosomal protein (rpS6) gene and determination of its location at chromosome 9p21.. Human molecular genetics, [PMID:1301164] |
| 7. Pata, I I, Hoth, S S, Kruppa, J J and Metspalu, A A.. (1992) The human ribosomal protein S6 gene: isolation, primary structure and location in chromosome 9.. Gene, (16): [PMID:1446836] |
| 8. Lott, J B JB and Mackie, G A GA.. (1988) Isolation and characterization of cloned cDNAs that code for human ribosomal protein S6.. Gene, (15): [PMID:2840355] |
| 9. Heinze, H H, Arnold, H H HH, Fischer, D D and Kruppa, J J.. (1988) The primary structure of the human ribosomal protein S6 derived from a cloned cDNA.. The Journal of biological chemistry, (25): [PMID:3279029] |
| 10. Vladimirov, S N SN and 7 more authors.. (1996) Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry.. European journal of biochemistry, (1): [PMID:8706699] |
| 11. Yamashita, Daisuke D and 8 more authors.. (2007) hDREF regulates cell proliferation and expression of ribosomal protein genes.. Molecular and cellular biology, [PMID:17220279] |
| 12. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 13. Stevens, Craig C and 6 more authors.. (2009) Peptide combinatorial libraries identify TSC2 as a death-associated protein kinase (DAPK) death domain-binding protein and reveal a stimulatory role for DAPK in mTORC1 signaling.. The Journal of biological chemistry, (2): [PMID:18974095] |
| 14. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 15. Schläfli, Philipp P and 5 more authors.. (2011) Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN.. The FEBS journal, [PMID:21418524] |
| 16. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 17. Anger, Andreas M AM and 6 more authors.. (2013) Structures of the human and Drosophila 80S ribosome.. Nature, (2): [PMID:23636399] |
| 18. Ban, Nenad N and 24 more authors.. (2014) A new system for naming ribosomal proteins.. Current opinion in structural biology, [PMID:24524803] |
| 19. Wilkins, Sarah E SE and 7 more authors.. (2018) JMJD5 is a human arginyl C-3 hydroxylase.. Nature communications, (21): [PMID:29563586] |
