Recombinant RSK3 Antibody - Primary antibody, specific to RPS6KA2, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human RSK3 Antibody; WB, IHC, ICC, IF; Unconjugated

  • Species reactivity(Reacts with): Hamster,Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • IHC
  • WB
Item Number
Ab126068
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab126068-10μl
10μl
In stock
$66.90
Ab126068-50μl
50μl
In stock
$199.90
Ab126068-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab126068-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

Recombinant; Rabbit anti Human RSK3 Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant RSK3 Antibody - Primary antibody, specific to RPS6KA2, Rabbit IgG
Synonyms90 kDa ribosomal protein S6 kinase 2 antibody | HU 2 antibody | KS6A2_HUMAN antibody | MAP kinase activated protein kinase 1c antibody | MAP kinase-activated protein kinase 1c antibody | MAPK-activated protein kinase 1c antibody | MAPKAP kinase 1c antibod
Specifications & PurityExactAb™, Validated, Recombinant, 0.6 mg/mL
Host speciesRabbit
SpecificityRPS6KA2
ImmunogenA synthetic peptide derived from human RSK3 (AA 343-362)
Positive ControlWB: C6, NH/3T3, A431, K562 and HEK293 cell lysates. IHC: Human lung cancer tissue. ICC/IF: U87-MG.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human RSK3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.
Post-translational
Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1. N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.6 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant RSK3 Antibody (Ab126068) - Western Blot
All lanes: Recombinant RSK3 Antibody (Ab126068) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 83 kDa
Observed band size: 84 kDa
Exposure time: 11.1 s

Recombinant RSK3 Antibody (Ab126068) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant RSK3 Antibody (Ab126068). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Recombinant RSK3 Antibody (Ab126068) - IF/ICC
Immunocytochemistry analysis of RSK3 (green) in U87-MG using Recombinant RSK3 Antibody (Ab126068), and cells were counterstained with DAPI (blue).


Associated Targets(Human)

RPS6KA2 Tchem Ribosomal protein S6 kinase alpha-2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
RPS6KA2 Tchem Ribosomal protein S6 kinase alpha 2 (2184 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
WB

1/500-1/1000

ICC/IF

1/50-1/200

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0102516Certificate of AnalysisJan 18, 2024 Ab126068
ZJ24F0102517Certificate of AnalysisJan 18, 2024 Ab126068

Related Documents

References

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