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Basic Description

Product Name	Recombinant RUNX2 Antibody - Primary antibody, specific to RUNX2, Rabbit IgG
Synonyms	AML3 \| CCD \| Acute myeloid leukemia 3 protein \| CBFA1 \| CBF-alpha-1 \| CCD1 \| CCDAML3 \| CLCD \| Core-binding factor subunit alpha-1 \| core-binding factor, runt domain, alpha subunit 1 \| MGC120023 \| ML3 \| oncogene AML-3 \| OSF2 \| OSF-2 \| osteoblast-specific t
Specifications & Purity	Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms	Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhanc
Host species	Rabbit
Specificity	RUNX2
Immunogen	A synthetic peptide derived from human RUNX2 (AA 300-450)
Positive Control	WB: MDA-MB-231 cell lysate. IHC: Human colon, Human Hodgkin's lymphoma tissue. IF/ICC: Saos-2 cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human RUNX2 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant RUNX2 Antibody (Ab126131)

Recombinant RUNX2 Antibody (Ab126131) - Western Blot
All lanes: Recombinant RUNX2 Antibody (Ab126131) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 57 kDa
Observed band size: 59 kDa. The RUNX2 protein, also known as Core-binding factor 1, is a transcription factor that plays a crucial role in cell differentiation and skeletal development. Transcription factors with DNA-binding motifs influence migration resulting in the generation of multiple bands.
Exposure time: 8.1 s

IF/ICC: Recombinant RUNX2 Antibody (Ab126131)

Recombinant RUNX2 Antibody (Ab126131) - IF/ICC
Immunofluorescent analysis of Saos-2 cells, using Recombinant RUNX2 Antibody (Ab126131).

IHC: Recombinant RUNX2 Antibody (Ab126131)

Recombinant RUNX2 Antibody (Ab126131) - IHC
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using Recombinant RUNX2 Antibody (Ab126131) at 1/300 dilution.

IHC: Recombinant RUNX2 Antibody (Ab126131)

Recombinant RUNX2 Antibody (Ab126131) - IHC
Immunohistochemical analysis of paraffin-embedded human colon, using Recombinant RUNX2 Antibody (Ab126131).

Associated Targets(Human)

RUNX2 Tbio Runt-related transcription factor 2 (0 Activities)

Discover Target: RUNX2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
IHC	1/100-1/500 "> 1/100-1/500
WB	1/1000 - 1/2000 "> 1/1000 - 1/2000
IF/ICC	1/50 - 1/200 "> 1/50 - 1/200

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0708451	Certificate of Analysis	Jul 17, 2024	Ab126131
ZJ24F0708450	Certificate of Analysis	Jul 17, 2024	Ab126131

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References

1. Mundlos, S S and 13 more authors.. (1997) Mutations involving the transcription factor CBFA1 cause cleidocranial dysplasia.. Cell, (30): [PMID:9182765]
2. Lee, B B and 8 more authors.. (1997) Missense mutations abolishing DNA binding of the osteoblast-specific transcription factor OSF2/CBFA1 in cleidocranial dysplasia.. Nature genetics, [PMID:9207800]
3. Zhang, Y W YW, Bae, S C SC, Takahashi, E E and Ito, Y Y.. (1997) The cDNA cloning of the transcripts of human PEBP2alphaA/CBFA1 mapped to 6p12.3-p21.1, the locus for cleidocranial dysplasia.. Oncogene, (17): [PMID:9233771]
4. Geoffroy, V V, Corral, D A DA, Zhou, L L, Lee, B B and Karsenty, G G.. (1998) Genomic organization, expression of the human CBFA1 gene, and evidence for an alternative splicing event affecting protein function.. Mammalian genome : official journal of the International Mammalian Genome Society, [PMID:9434946]
5. Xiao, Z S ZS, Thomas, R R, Hinson, T K TK and Quarles, L D LD.. (1998) Genomic structure and isoform expression of the mouse, rat and human Cbfa1/Osf2 transcription factor.. Gene, (3): [PMID:9651525]
6. Quack, I I and 12 more authors.. (1999) Mutation analysis of core binding factor A1 in patients with cleidocranial dysplasia.. American journal of human genetics, [PMID:10521292]
7. Zhou, G G and 11 more authors.. (1999) CBFA1 mutation analysis and functional correlation with phenotypic variability in cleidocranial dysplasia.. Human molecular genetics, [PMID:10545612]
8. Zhang, Y W YW and 13 more authors.. (2000) PEBP2alphaA/CBFA1 mutations in Japanese cleidocranial dysplasia patients.. Gene, (22): [PMID:10689183]
9. Giannotti, A A and 7 more authors.. (2000) A novel CBFA1 mutation (R190W) in an Italian family with cleidocranial dysplasia.. Human mutation, [PMID:10980549]
10. Otto, Florian F, Kanegane, Hirokazu H and Mundlos, Stefan S.. (2002) Mutations in the RUNX2 gene in patients with cleidocranial dysplasia.. Human mutation, [PMID:11857736]
11. Pelletier, Nadine N, Champagne, Nathalie N, Stifani, Stefano S and Yang, Xiang-Jiao XJ.. (2002) MOZ and MORF histone acetyltransferases interact with the Runt-domain transcription factor Runx2.. Oncogene, (18): [PMID:11965546]
12. Machuca-Tzili, L L, Monroy-Jaramillo, N N, González-del Angel, A A and Kofman-Alfaro, S S.. (2002) New mutations in the CBFA1 gene in two Mexican patients with cleidocranial dysplasia.. Clinical genetics, [PMID:12081718]
13. Willis, David M DM and 5 more authors.. (2002) Regulation of osteocalcin gene expression by a novel Ku antigen transcription factor complex.. The Journal of biological chemistry, (4): [PMID:12145306]
14. Yoshida, Taketoshi T and 6 more authors.. (2002) Functional analysis of RUNX2 mutations in Japanese patients with cleidocranial dysplasia demonstrates novel genotype-phenotype correlations.. American journal of human genetics, [PMID:12196916]
15. Morava, Eva E and 5 more authors.. (2002) Cleidocranial dysplasia with decreased bone density and biochemical findings of hypophosphatasia.. European journal of pediatrics, [PMID:12424590]
16. Kim, Hyo-Jin HJ and 14 more authors.. (2006) Four novel RUNX2 mutations including a splice donor site result in the cleidocranial dysplasia phenotype.. Journal of cellular physiology, [PMID:16270353]
17. Qiao, Meng M and 5 more authors.. (2006) Cell cycle-dependent phosphorylation of the RUNX2 transcription factor by cdc2 regulates endothelial cell proliferation.. The Journal of biological chemistry, (17): [PMID:16407259]
18. Ono, Masahiro M and 8 more authors.. (2007) Foxp3 controls regulatory T-cell function by interacting with AML1/Runx1.. Nature, (5): [PMID:17377532]
19. Ryoo, H-M HM, Kang, H-Y HY, Lee, S-K SK, Lee, K-E KE and Kim, J-W JW.. (2010) RUNX2 mutations in cleidocranial dysplasia patients.. Oral diseases, [PMID:19744171]
20. Wang, G X GX, Sun, R P RP and Song, F L FL.. (2010) A novel RUNX2 mutation (T420I) in Chinese patients with cleidocranial dysplasia.. Genetics and molecular research : GMR, (12): [PMID:20082269]
21. Ott, Claus E CE and 18 more authors.. (2010) Deletions of the RUNX2 gene are present in about 10% of individuals with cleidocranial dysplasia.. Human mutation, [PMID:20648631]
22. Moffatt, Pierre P and 14 more authors.. (2013) Metaphyseal dysplasia with maxillary hypoplasia and brachydactyly is caused by a duplication in RUNX2.. American journal of human genetics, (7): [PMID:23290074]
23. Callea, Michele M and 7 more authors.. (2014) A case of cleidocranial dysplasia with peculiar dental features: pathogenetic role of the RUNX2 mutation and long term follow-up.. Oral health and dental management, [PMID:24984680]
24. Zeng, Li L and 8 more authors.. (2017) Functional analysis of novel RUNX2 mutations in cleidocranial dysplasia.. Mutagenesis, (1): [PMID:28505335]
25. Jung, Yu-Jin YJ, Bae, Han-Sol HS, Ryoo, Hyun-Mo HM and Baek, Seung-Hak SH.. (2018) A novel RUNX2 mutation in exon 8, G462X, in a patient with Cleidocranial Dysplasia.. Journal of cellular biochemistry, [PMID:28703881]
26. Zhang, Xianli X and 5 more authors.. (2017) Analysis of novel RUNX2 mutations in Chinese patients with cleidocranial dysplasia.. PloS one, [PMID:28738062]

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SKU	Size	Availability	Price	Qty
Ab126131-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$49.90	+-
Ab126131-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$149.90	+-
Ab126131-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$249.90	+-
Ab126131-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,399.90	+-

Recombinant RUNX2 Antibody - Primary antibody, specific to RUNX2, Rabbit IgG