Recombinant S100 alpha 6/PRA Antibody - Primary antibody, specific to S100 alpha 6/PRA, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human S100 alpha 6/PRA Antibody; WB, ICC, IF; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • WB
Item Number
Ab126179
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab126179-10μl
10μl
In stock
$69.90
Ab126179-50μl
50μl
In stock
$189.90
Ab126179-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab126179-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Recombinant; Rabbit anti Human S100 alpha 6/PRA Antibody; WB, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant S100 alpha 6/PRA Antibody - Primary antibody, specific to S100 alpha 6/PRA, Rabbit IgG
Synonyms2A9 antibody | 5B10 antibody | CABP antibody | CACY antibody | Calcyclin antibody | Growth factor inducible protein 2A9 antibody | Growth factor-inducible protein 2A9 antibody | MLN 4 antibody | MLN4 antibody | OTTHUMP00000015472antibody | OTTHUMP00000015
Specifications & PurityExactAb™, Validated, Recombinant, 0.3 mg/mL
Host speciesRabbit
SpecificityS100 alpha 6/PRA
ImmunogenRecombinant full length protein (Human)
Positive ControlWB: HeLa, A549, HUVEC, RAW 264.7 and NIH/3T3 cell lysates. ICC/IF: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human S100 alpha 6/PRA Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function:
May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.3 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant S100 alpha 6/PRA Antibody (Ab126179) - Western Blot
All lanes: Recombinant S100 alpha 6/PRA Antibody (Ab126179) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 10 kDa
Observed band size: 12 kDa
Exposure time: 11.5 s

Recombinant S100 alpha 6/PRA Antibody (Ab126179) - Western Blot
All lanes: Recombinant S100 alpha 6/PRA Antibody (Ab126179) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 10 kDa
Observed band size: 12 kDa
Exposure time: 13.9 s

Recombinant S100 alpha 6/PRA Antibody (Ab126179) - IF/ICC
Immunocytochemistry analysis of S100 alpha 6/PRA (green) in HeLa using Recombinant S100 alpha 6/PRA Antibody (Ab126179), and cells were counterstained with DAPI (blue).

Associated Targets(Human)

S100A6 Tbio Protein S100-A6 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

ICC/IF

1/50-1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0403975Certificate of AnalysisApr 01, 2024 Ab126179
ZJ24F0403974Certificate of AnalysisApr 01, 2024 Ab126179

Related Documents

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
2. Tomida, Y Y, Terasawa, M M, Kobayashi, R R and Hidaka, H H..  (1992)  Calcyclin and calvasculin exist in human platelets..  Biochemical and biophysical research communications,    (30):   [PMID:1482346]
3. Murphy, L C LC, Murphy, L J LJ, Tsuyuki, D D, Duckworth, M L ML and Shiu, R P RP..  (1988)  Cloning and characterization of a cDNA encoding a highly conserved, putative calcium binding protein, identified by an anti-prolactin receptor antiserum..  The Journal of biological chemistry,    (15):   [PMID:2448309]
4. Gabius, H J HJ and 5 more authors..  (1989)  Identification of a cell cycle-dependent gene product as a sialic acid-binding protein..  Biochemical and biophysical research communications,    (30):   [PMID:2775283]
5. Ferrari, S S and 9 more authors..  (1987)  Structural and functional analysis of a growth-regulated gene, the human calcyclin..  The Journal of biological chemistry,    (15):   [PMID:3036810]
6. Calabretta, B B, Battini, R R, Kaczmarek, L L, de Riel, J K JK and Baserga, R R..  (1986)  Molecular cloning of the cDNA for a growth factor-inducible gene with strong homology to S-100, a calcium-binding protein..  The Journal of biological chemistry,    (25):   [PMID:3755724]
7. Otterbein, Ludovic R LR, Kordowska, Jolanta J, Witte-Hoffmann, Carlos C, Wang, C-L Albert CL and Dominguez, Roberto R..  (2002)  Crystal structures of S100A6 in the Ca(2+)-free and Ca(2+)-bound states: the calcium sensor mechanism of S100 proteins revealed at atomic resolution..  Structure (London, England : 1993),      [PMID:11937060]
8. Tomas, Alejandra A and Moss, Stephen E SE..  (2003)  Calcium- and cell cycle-dependent association of annexin 11 with the nuclear envelope..  The Journal of biological chemistry,    (30):   [PMID:12601007]
9. Nowotny, Marcin M and 5 more authors..  (2003)  Calcium-regulated interaction of Sgt1 with S100A6 (calcyclin) and other S100 proteins..  The Journal of biological chemistry,    (18):   [PMID:12746458]
10. Gregory, S G SG and 178 more authors..  (2006)  The DNA sequence and biological annotation of human chromosome 1..  Nature,    (18):   [PMID:16710414]
11. Nedjadi, T T and 9 more authors..  (2009)  S100A6 binds to annexin 2 in pancreatic cancer cells and promotes pancreatic cancer cell motility..  British journal of cancer,    (6):   [PMID:19724273]
12. van Dieck, Jan J and 6 more authors..  (2009)  Posttranslational modifications affect the interaction of S100 proteins with tumor suppressor p53..  Journal of molecular biology,    (18):   [PMID:19819244]
13. Shimamoto, Seiko S, Kubota, Yasuo Y, Tokumitsu, Hiroshi H and Kobayashi, Ryoji R..  (2010)  S100 proteins regulate the interaction of Hsp90 with Cyclophilin 40 and FKBP52 through their tetratricopeptide repeats..  FEBS letters,    (19):   [PMID:20188096]
14. Yamaguchi, Fuminori F and 6 more authors..  (2012)  S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation..  The Journal of biological chemistry,    (20):   [PMID:22399290]
15. Smith, Bradley N BN and 55 more authors..  (2017)  Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis..  Science translational medicine,    (3):   [PMID:28469040]

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