| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab126179-10μl | 10μl | In stock | $69.90 | |
| Ab126179-50μl | 50μl | In stock | $189.90 | |
| Ab126179-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab126179-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human S100 alpha 6/PRA Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant S100 alpha 6/PRA Antibody - Primary antibody, specific to S100 alpha 6/PRA, Rabbit IgG |
|---|---|
| Synonyms | 2A9 antibody | 5B10 antibody | CABP antibody | CACY antibody | Calcyclin antibody | Growth factor inducible protein 2A9 antibody | Growth factor-inducible protein 2A9 antibody | MLN 4 antibody | MLN4 antibody | OTTHUMP00000015472antibody | OTTHUMP00000015 |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | S100 alpha 6/PRA |
| Immunogen | Recombinant full length protein (Human) |
| Positive Control | WB: HeLa, A549, HUVEC, RAW 264.7 and NIH/3T3 cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human S100 alpha 6/PRA Antibody, Recombinant, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant S100 alpha 6/PRA Antibody (Ab126179) - Western Blot
All lanes: Recombinant S100 alpha 6/PRA Antibody (Ab126179) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 10 kDa
Observed band size: 12 kDa
Exposure time: 11.5 s
Recombinant S100 alpha 6/PRA Antibody (Ab126179) - Western Blot
All lanes: Recombinant S100 alpha 6/PRA Antibody (Ab126179) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 10 kDa
Observed band size: 12 kDa
Exposure time: 13.9 s
Recombinant S100 alpha 6/PRA Antibody (Ab126179) - IF/ICC
Immunocytochemistry analysis of S100 alpha 6/PRA (green) in HeLa using Recombinant S100 alpha 6/PRA Antibody (Ab126179), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0403975 | Certificate of Analysis | Apr 01, 2024 | Ab126179 |
| ZJ24F0403974 | Certificate of Analysis | Apr 01, 2024 | Ab126179 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Tomida, Y Y, Terasawa, M M, Kobayashi, R R and Hidaka, H H.. (1992) Calcyclin and calvasculin exist in human platelets.. Biochemical and biophysical research communications, (30): [PMID:1482346] |
| 3. Murphy, L C LC, Murphy, L J LJ, Tsuyuki, D D, Duckworth, M L ML and Shiu, R P RP.. (1988) Cloning and characterization of a cDNA encoding a highly conserved, putative calcium binding protein, identified by an anti-prolactin receptor antiserum.. The Journal of biological chemistry, (15): [PMID:2448309] |
| 4. Gabius, H J HJ and 5 more authors.. (1989) Identification of a cell cycle-dependent gene product as a sialic acid-binding protein.. Biochemical and biophysical research communications, (30): [PMID:2775283] |
| 5. Ferrari, S S and 9 more authors.. (1987) Structural and functional analysis of a growth-regulated gene, the human calcyclin.. The Journal of biological chemistry, (15): [PMID:3036810] |
| 6. Calabretta, B B, Battini, R R, Kaczmarek, L L, de Riel, J K JK and Baserga, R R.. (1986) Molecular cloning of the cDNA for a growth factor-inducible gene with strong homology to S-100, a calcium-binding protein.. The Journal of biological chemistry, (25): [PMID:3755724] |
| 7. Otterbein, Ludovic R LR, Kordowska, Jolanta J, Witte-Hoffmann, Carlos C, Wang, C-L Albert CL and Dominguez, Roberto R.. (2002) Crystal structures of S100A6 in the Ca(2+)-free and Ca(2+)-bound states: the calcium sensor mechanism of S100 proteins revealed at atomic resolution.. Structure (London, England : 1993), [PMID:11937060] |
| 8. Tomas, Alejandra A and Moss, Stephen E SE.. (2003) Calcium- and cell cycle-dependent association of annexin 11 with the nuclear envelope.. The Journal of biological chemistry, (30): [PMID:12601007] |
| 9. Nowotny, Marcin M and 5 more authors.. (2003) Calcium-regulated interaction of Sgt1 with S100A6 (calcyclin) and other S100 proteins.. The Journal of biological chemistry, (18): [PMID:12746458] |
| 10. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 11. Nedjadi, T T and 9 more authors.. (2009) S100A6 binds to annexin 2 in pancreatic cancer cells and promotes pancreatic cancer cell motility.. British journal of cancer, (6): [PMID:19724273] |
| 12. van Dieck, Jan J and 6 more authors.. (2009) Posttranslational modifications affect the interaction of S100 proteins with tumor suppressor p53.. Journal of molecular biology, (18): [PMID:19819244] |
| 13. Shimamoto, Seiko S, Kubota, Yasuo Y, Tokumitsu, Hiroshi H and Kobayashi, Ryoji R.. (2010) S100 proteins regulate the interaction of Hsp90 with Cyclophilin 40 and FKBP52 through their tetratricopeptide repeats.. FEBS letters, (19): [PMID:20188096] |
| 14. Yamaguchi, Fuminori F and 6 more authors.. (2012) S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation.. The Journal of biological chemistry, (20): [PMID:22399290] |
| 15. Smith, Bradley N BN and 55 more authors.. (2017) Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis.. Science translational medicine, (3): [PMID:28469040] |
