| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab126219-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab126219-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $189.90 | |
| Ab126219-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab126219-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,099.90 |
mAb (6F4E6D5C10); Mouse anti Human S100A10 Antibody; WB, IF/ICC; Unconjugated
| Product Name | Recombinant S100A10 Antibody - Primary antibody, specific to S100A10, Rabbit IgG |
|---|---|
| Synonyms | Calpactin I light chain | Calpactin-1 light chain | Cellular ligand of annexin II | p10 protein | p11 | S100 calcium-binding protein A10 | 42C antibody | AA409961 antibody | AL024248 antibody | Annexin II ligand antibody | Annexin II ligand, calpactin I, |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase. |
| Host species | Mouse |
| Specificity | S100A10 |
| Immunogen | Recombinant protein of mouse S100A10. |
| Positive Control | WB: C6, NIH3T3, HeLa lysates. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Mouse anti Human S100A10 Antibody, Monoclonal (6F4E6D5C10), could be used for WB, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
S100A10 Mouse mAb (Ab126219) - Western Blot
All lanes: S100A10 Mouse mAb (Ab126219) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa
Exposure time: 58.4 s
S100A10 Mouse mAb (Ab126219) - IF/ICC
Immunofluorescent analysis of S100-A10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with S100A10 Mouse mAb (Ab126219) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC | 1/50-1/100 |
Enter Lot Number to search for COA:
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Reddy TR, Li C, Guo X, Myrvang HK, Fischer PM, Dekker LV.. (2011) Design, synthesis, and structure-activity relationship exploration of 1-substituted 4-aroyl-3-hydroxy-5-phenyl-1H-pyrrol-2(5H)-one analogues as inhibitors of the annexin A2-S100A10 protein interaction.. J Med Chem, 54 (7): (2080-2094). [PMID:21375334] [10.1021/jm101212e] |
| 3. Dooley, T P TP, Weiland, K L KL and Simon, M M.. (1992) cDNA sequence of human p11 calpactin I light chain.. Genomics, [PMID:1386341] |
| 4. Harder, T T, Kube, E E and Gerke, V V.. (1992) Cloning and characterization of the human gene encoding p11: structural similarity to other members of the S-100 gene family.. Gene, (15): [PMID:1533380] |
| 5. Kube, E E, Weber, K K and Gerke, V V.. (1991) Primary structure of human, chicken, and Xenopus laevis p11, a cellular ligand of the Src-kinase substrate, annexin II.. Gene, (30): [PMID:1831433] |
| 6. Réty, S S and 7 more authors.. (1999) The crystal structure of a complex of p11 with the annexin II N-terminal peptide.. Nature structural biology, [PMID:9886297] |
| 7. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 8. Mi, Lixin and 8 more authors.. (2011) Identification of potential protein targets of isothiocyanates by proteomics.. Chemical research in toxicology, (17): [PMID:21838287] |
