| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab126240-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab126240-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $189.90 | |
| Ab126240-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab126240-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human S100A4 Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | Recombinant S100A4 Antibody - Primary antibody, specific to S100A4, Rabbit IgG |
|---|---|
| Synonyms | Calvasculin | Metastasin | Placental calcium-binding protein | Protein Mts1 | S100 calcium-binding protein A4 | 18A2 | 42A | Calvasculin | CAPL | CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin | fibroblast-specific protein-1 |
| Specifications & Purity | Recombinant, ExactAb™, Validated, Lot by Lot |
| Host species | Rabbit |
| Specificity | S100A4 |
| Immunogen | A synthetic peptide derived from human S100A4 (AA 1-101) |
| Positive Control | WB: THP-1 cell lysate. IHC: Rat stomach, Human lung adenocarcinoma, thyroid cancer, esophageal carcinoma, colon carcinoma tissues. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human S100A4 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant S100A4 Antibody (Ab126240) - Western Blot
All lanes: Recombinant S100A4 Antibody (Ab126240) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
Exposure time: 100.0 s
Recombinant S100A4 Antibody (Ab126240) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant S100A4 Antibody (Ab126240). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant S100A4 Antibody (Ab126240) - IF/ICC
Immunocytochemistry analysis of S1A4 (green) in HeLa using Recombinant S100A4 Antibody (Ab126240) and DAPI(blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 "> 1/100 - 1/200 |
| WB | 1/1000 - 1/2000"> 1/1000 - 1/2000 |
| IF/ICC | 1/50 - 1/200 "> 1/50 - 1/200 |
Enter Lot Number to search for COA:
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Tulchinsky, E E and 6 more authors.. (1992) Transcriptional analysis of the mts1 gene with specific reference to 5' flanking sequences.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1329089] |
| 3. Engelkamp, D D, Schäfer, B W BW, Erne, P P and Heizmann, C W CW.. (1992) S100 alpha, CAPL, and CACY: molecular cloning and expression analysis of three calcium-binding proteins from human heart.. Biochemistry, (27): [PMID:1384693] |
| 4. Tomida, Y Y, Terasawa, M M, Kobayashi, R R and Hidaka, H H.. (1992) Calcyclin and calvasculin exist in human platelets.. Biochemical and biophysical research communications, (30): [PMID:1482346] |
| 5. Engelkamp, D D, Schäfer, B W BW, Mattei, M G MG, Erne, P P and Heizmann, C W CW.. (1993) Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:8341667] |
| 6. Kriajevska, Marina M and 7 more authors.. (2002) Liprin beta 1, a member of the family of LAR transmembrane tyrosine phosphatase-interacting proteins, is a new target for the metastasis-associated protein S100A4 (Mts1).. The Journal of biological chemistry, (15): [PMID:11836260] |
| 7. Vallely, Kristen M KM and 5 more authors.. (2002) Solution structure of human Mts1 (S100A4) as determined by NMR spectroscopy.. Biochemistry, (22): [PMID:12379109] |
| 8. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 9. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 10. Malashkevich, Vladimir N VN and 9 more authors.. (2010) Phenothiazines inhibit S100A4 function by inducing protein oligomerization.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:20421509] |
| 11. Haase-Kohn, Cathleen C, Wolf, Susann S, Lenk, Jens J and Pietzsch, Jens J.. (2011) Copper-mediated cross-linking of S100A4, but not of S100A2, results in proinflammatory effects in melanoma cells.. Biochemical and biophysical research communications, (30): [PMID:21924240] |
| 12. Li, Xiaoli X and 7 more authors.. (2012) The unique cytoplasmic domain of human FcγRIIIA regulates receptor-mediated function.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:23024279] |
| 13. Orre, L M LM and 6 more authors.. (2013) S100A4 interacts with p53 in the nucleus and promotes p53 degradation.. Oncogene, (5): [PMID:23752197] |
| 14. Dukhanina, E A EA and 7 more authors.. (2015) A new role for PGRP-S (Tag7) in immune defense: lymphocyte migration is induced by a chemoattractant complex of Tag7 with Mts1.. Cell cycle (Georgetown, Tex.), [PMID:26654597] |
