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Recombinant S6K1 Antibody - Primary antibody, specific to RPS6KB1, Rabbit IgG

Recombinant

Validated

ExactAb™

0.2 mg/mL

Application:

IF/ICC
WB

Features and benefits

Host species: Rabbit
Species reactivity(Reacts with): Human,Mouse,Rat
Isotype: Rabbit IgG
Conjugation: Unconjugated

Item Number

Ab126314

Grouped product items
SKU	Size	Availability	Price
Ab126314-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$66.90
Ab126314-50μl	50μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$199.90
Ab126314-100μl	100μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$319.90
Ab126314-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,699.90

Recombinant; Rabbit anti Human S6K1 Antibody; WB, ICC, IF; Unconjugated

View related series

Click to view target information: RPS6KB1

Accession#:P23443 Gene ID:6198 RPS6KB1 S6K1

Basic Description

Product Name	Recombinant S6K1 Antibody - Primary antibody, specific to RPS6KB1, Rabbit IgG
Synonyms	RPS6KB1 \| STK14A \| Ribosomal protein S6 kinase beta-1 \| S6K-beta-1 \| S6K1 \| 70 kDa ribosomal protein S6 kinase 1 \| P70S6K1 \| p70-S6K 1 \| Ribosomal protein S6 kinase I \| Serine/threonine-protein kinase 14A \| p70 ribosomal S6 kinase alpha \| p70 S6 kinas \| 7
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species	Rabbit
Specificity	RPS6KB1
Immunogen	A synthetic peptide derived from human S6K1 (AA 1-17).
Positive Control	WB: Raw264.7, NIH/3T3, A549, A431, C6 and K562 cell lysates. ICC/IF: HeLa cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human S6K1 Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Immunogen affinity purified
Form	Liquid
Concentration	0.2 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant S6K1 Antibody (Ab126314) - Western Blot
All lanes: Recombinant S6K1 Antibody (Ab126314) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 63 kDa
Exposure time: 1.0 s

Recombinant S6K1 Antibody (Ab126314) - IF
Immunocytochemistry analysis of S6K1 (green) in HeLa cells using Recombinant S6K1 Antibody (Ab126314) and cells were counterstained with DAPI (blue).

Associated Targets(Human)

RPS6KB1 Tchem Ribosomal protein S6 kinase beta-1 (0 Activities)

Discover Target: RPS6KB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

RPS6KB1 Tchem Ribosomal protein S6 kinase 1 (4456 Activities)

Discover Target: RPS6KB1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Application

Dilution info

1/500-1/1000

IF/ICC

1/50-1/200

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0901069	Certificate of Analysis	Sep 18, 2023	Ab126314
ZJ23F0901068	Certificate of Analysis	Sep 18, 2023	Ab126314

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