Recombinant SA2 Antibody - Primary antibody, specific to STAG2, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human SA2 Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab126330
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab126330-10μl
10μl
In stock
$79.90
Ab126330-50μl
50μl
In stock
$219.90
Ab126330-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$339.90
Ab126330-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human SA2 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant SA2 Antibody - Primary antibody, specific to STAG2, Rabbit IgG
SynonymsSCC3 homolog 2 | Stromal antigen 2 | bA517O1.1 antibody | Cohesin Subunit SA 2 antibody | Cohesin subunit SA-2 antibody | DKFZp686P168 antibody | DKFZp781H1753 antibody | FLJ25871 antibody | SA 2 antibody | SA-2 antibody | SA2 antibody | SCC3 homolog 2 an
Specifications & PurityExactAb™, Validated, Recombinant, 0.5 mg/mL
Host speciesRabbit
SpecificitySTAG2
ImmunogenA synthetic peptide derived from human SA2 (AA 1000-1231)
Positive ControlWB: K562, Hep G2, A431 and MOLT-4 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human SA2 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Protein Function
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

Product Properties

IsotypeRabbit IgG
Light Chain TypeKappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SA2 Antibody (Ab126330) - Western Blot
All lanes: Recombinant SA2 Antibody (Ab126330) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 141 kDa
Observed band size: 147 kDa
Exposure time: 67.99 s


Recombinant SA2 Antibody (Ab126330) - Western Blot
All lanes: Recombinant SA2 Antibody (Ab126330) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 141 kDa
Observed band size: 147 kDa
Exposure time: 18.8 s

Associated Targets(Human)

STAG2 Tbio Cohesin subunit SA-2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/2000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0405045Certificate of AnalysisApr 30, 2024 Ab126330
ZJ24F0405044Certificate of AnalysisApr 30, 2024 Ab126330

Related Documents

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
2. Carramolino, L L and 13 more authors..  (1997)  SA-1, a nuclear protein encoded by one member of a novel gene family: molecular cloning and detection in hemopoietic organs..  Gene,    (22):   [PMID:9305759]
3. Sumara, I I, Vorlaufer, E E, Gieffers, C C, Peters, B H BH and Peters, J M JM..  (2000)  Characterization of vertebrate cohesin complexes and their regulation in prophase..  The Journal of cell biology,    (13):   [PMID:11076961]
4. Prieto, Ignacio I and 11 more authors..  (2002)  STAG2 and Rad21 mammalian mitotic cohesins are implicated in meiosis..  EMBO reports,      [PMID:12034751]
5. Ross, Mark T MT and 281 more authors..  (2005)  The DNA sequence of the human X chromosome..  Nature,    (17):   [PMID:15772651]
6. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
7. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,    (15):   [PMID:21406692]
8. Mullegama, Sureni V SV and 11 more authors..  (2017)  De novo loss-of-function variants in STAG2 are associated with developmental delay, microcephaly, and congenital anomalies..  American journal of medical genetics. Part A,      [PMID:28296084]
9. Soardi, Fernanda C FC and 16 more authors..  (2017)  Familial STAG2 germline mutation defines a new human cohesinopathy..  NPJ genomic medicine,      [PMID:29263825]
10. Yuan, Bo B and 46 more authors..  (2019)  Clinical exome sequencing reveals locus heterogeneity and phenotypic variability of cohesinopathies..  Genetics in medicine : official journal of the American College of Medical Genetics,      [PMID:30158690]
11. Mullegama, Sureni V SV and 5 more authors..  (2019)  Mutations in STAG2 cause an X-linked cohesinopathy associated with undergrowth, developmental delay, and dysmorphia: Expanding the phenotype in males..  Molecular genetics & genomic medicine,      [PMID:30447054]
12. Aoi, Hiromi H and 12 more authors..  (2019)  Nonsense variants in STAG2 result in distinct sex-dependent phenotypes..  Journal of human genetics,      [PMID:30765867]
13. Kruszka, Paul P and 34 more authors..  (2019)  Cohesin complex-associated holoprosencephaly..  Brain : a journal of neurology,    (23):   [PMID:31334757]

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