A synthetic peptide derived from human SA2 (AA 1000-1231)
Positive Control
WB: K562, Hep G2, A431 and MOLT-4 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human SA2 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/2000 Protein Function Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
Kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant SA2 Antibody (Ab126330) - Western Blot All lanes: Recombinant SA2 Antibody (Ab126330) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 141 kDa Observed band size: 147 kDa Exposure time: 67.99 s
Recombinant SA2 Antibody (Ab126330) - Western Blot All lanes: Recombinant SA2 Antibody (Ab126330) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 141 kDa Observed band size: 147 kDa Exposure time: 18.8 s
1.Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942):(834-40). [PMID:19608861]
2.Carramolino, L L and 13 more authors.. (1997) SA-1, a nuclear protein encoded by one member of a novel gene family: molecular cloning and detection in hemopoietic organs.. Gene, (22):[PMID:9305759]
3.Sumara, I I, Vorlaufer, E E, Gieffers, C C, Peters, B H BH and Peters, J M JM.. (2000) Characterization of vertebrate cohesin complexes and their regulation in prophase.. The Journal of cell biology, (13):[PMID:11076961]
4.Prieto, Ignacio I and 11 more authors.. (2002) STAG2 and Rad21 mammalian mitotic cohesins are implicated in meiosis.. EMBO reports, [PMID:12034751]
5.Ross, Mark T MT and 281 more authors.. (2005) The DNA sequence of the human X chromosome.. Nature, (17):[PMID:15772651]
6.Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18):[PMID:19690332]
7.Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15):[PMID:21406692]
8.Mullegama, Sureni V SV and 11 more authors.. (2017) De novo loss-of-function variants in STAG2 are associated with developmental delay, microcephaly, and congenital anomalies.. American journal of medical genetics. Part A, [PMID:28296084]
9.Soardi, Fernanda C FC and 16 more authors.. (2017) Familial STAG2 germline mutation defines a new human cohesinopathy.. NPJ genomic medicine, [PMID:29263825]
10.Yuan, Bo B and 46 more authors.. (2019) Clinical exome sequencing reveals locus heterogeneity and phenotypic variability of cohesinopathies.. Genetics in medicine : official journal of the American College of Medical Genetics, [PMID:30158690]
11.Mullegama, Sureni V SV and 5 more authors.. (2019) Mutations in STAG2 cause an X-linked cohesinopathy associated with undergrowth, developmental delay, and dysmorphia: Expanding the phenotype in males.. Molecular genetics & genomic medicine, [PMID:30447054]
12.Aoi, Hiromi H and 12 more authors.. (2019) Nonsense variants in STAG2 result in distinct sex-dependent phenotypes.. Journal of human genetics, [PMID:30765867]
13.Kruszka, Paul P and 34 more authors.. (2019) Cohesin complex-associated holoprosencephaly.. Brain : a journal of neurology, (23):[PMID:31334757]
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