Recombinant SAFB Antibody - Primary antibody, specific to SAFB, Rabbit IgG
Synonyms
SAFB | HAP | HET | SAFB1 | Scaffold attachment factor B1 | SAF-B | SAF-B1 | HSP27 estrogen response element-TATA box-binding protein | HSP27 ERE-TATA-binding protein | DKFZp779C1727 antibody | glutathione S transferase fusion protein antibody | HAP antibo
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.5 mg/mL
Host species
Rabbit
Specificity
SAFB
Immunogen
A synthetic peptide derived from human SAFB (AA 902-915)
Positive Control
WB: Mouse brain, K562, and C6 lysates. IHC: Human breast cancer tissue. IF/ICC: 293 cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human SAFB Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/50-1/200 Protein Function Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant SAFB Antibody (Ab126368) - Western Blot All lanes: Recombinant SAFB Antibody (Ab126368) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 103 kDa Observed band size: 150 kDa Exposure time: 10.5 s
Recombinant SAFB Antibody (Ab126368) - Western Blot All lanes: Recombinant SAFB Antibody (Ab126368) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 103 kDa Observed band size: 149 kDa Exposure time: 92.8 s
Recombinant SAFB Antibody (Ab126368) - IF/ICC Immunocytochemistry analysis of SAFB1 (green) in 293 using Recombinant SAFB Antibody (Ab126368) and DAPI (blue).
Recombinant SAFB Antibody (Ab126368) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant SAFB Antibody (Ab126368). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.