Recombinant SAFB Antibody - Primary antibody, specific to SAFB, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human SAFB Antibody; WB, IHC, IF, ICC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • IHC
  • WB
Item Number
Ab126368
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab126368-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$89.90
Ab126368-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$269.90
Ab126368-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$379.90
Ab126368-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Recombinant; Rabbit anti Human SAFB Antibody; WB, IHC, IF, ICC; Unconjugated

Basic Description

Product NameRecombinant SAFB Antibody - Primary antibody, specific to SAFB, Rabbit IgG
SynonymsSAFB | HAP | HET | SAFB1 | Scaffold attachment factor B1 | SAF-B | SAF-B1 | HSP27 estrogen response element-TATA box-binding protein | HSP27 ERE-TATA-binding protein | DKFZp779C1727 antibody | glutathione S transferase fusion protein antibody | HAP antibo
Specifications & PurityExactAb™, Validated, Recombinant, 0.5 mg/mL
Host speciesRabbit
SpecificitySAFB
ImmunogenA synthetic peptide derived from human SAFB (AA 902-915)
Positive ControlWB: Mouse brain, K562, and C6 lysates. IHC: Human breast cancer tissue. IF/ICC: 293 cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human SAFB Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50-1/200
Protein Function
Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SAFB Antibody (Ab126368) - Western Blot
All lanes: Recombinant SAFB Antibody (Ab126368) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 150 kDa
Exposure time: 10.5 s

Recombinant SAFB Antibody (Ab126368) - Western Blot
All lanes: Recombinant SAFB Antibody (Ab126368) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 149 kDa
Exposure time: 92.8 s

Recombinant SAFB Antibody (Ab126368) - IF/ICC
Immunocytochemistry analysis of SAFB1 (green) in 293 using Recombinant SAFB Antibody (Ab126368) and DAPI (blue).

Recombinant SAFB Antibody (Ab126368) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant SAFB Antibody (Ab126368). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

SAFB Tbio Scaffold attachment factor B1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IHC

1/50 - 1/100

IF/ICC

1/50 - 1/200

WB

1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0607098Certificate of AnalysisJun 20, 2024 Ab126368
ZJ24F0607097Certificate of AnalysisJun 20, 2024 Ab126368

Related Documents

Solution Calculators