Recombinant; Rabbit anti Human SAMHD1 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab126421-10μl | 10μl | In stock | $59.90 | |
| Ab126421-50μl | 50μl | In stock | $179.90 | |
| Ab126421-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $279.90 | |
| Ab126421-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human SAMHD1 Antibody; WB; Unconjugated
| Product Name | Recombinant SAMHD1 Antibody - Primary antibody, specific to SAMHD1, Rabbit IgG |
|---|---|
| Synonyms | CHBL2 antibody | DCIP antibody | Dendritic cell derived IFNG induced protein antibody | Dendritic cell-derived IFNG-induced protein antibody | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody | dNTPase antibody | HD domain containing 1 ant |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.33 mg/mL |
| Host species | Rabbit |
| Specificity | SAMHD1 |
| Immunogen | A synthetic peptide derived from human SAMHD1 (AA 580-626) |
| Positive Control | WB: MCF7, K562, Ramos, Hep G2 and HeLa cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human SAMHD1 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.33 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant SAMHD1 Antibody (Ab126421) - Western Blot
All lanes: Recombinant SAMHD1 Antibody (Ab126421) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 70 kDa
Exposure time: 7.6 s
Recombinant SAMHD1 Antibody (Ab126421) - Western Blot
All lanes: Recombinant SAMHD1 Antibody (Ab126421) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 70 kDa
Exposure time: 10.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0405047 | Certificate of Analysis | Apr 30, 2024 | Ab126421 |
| ZJ24F0405046 | Certificate of Analysis | Apr 30, 2024 | Ab126421 |
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| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Li, N N, Zhang, W W and Cao, X X.. (2000) Identification of human homologue of mouse IFN-gamma induced protein from human dendritic cells.. Immunology letters, (1): [PMID:11064105] |
| 4. Wiemann, S S and 23 more authors.. (2001) Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.. Genome research, [PMID:11230166] |
| 5. Liao, Wupeng W, Bao, Zhang Z, Cheng, Chang C, Mok, Yu-Keung YK and Wong, W S Fred WS.. (2008) Dendritic cell-derived interferon-gamma-induced protein mediates tumor necrosis factor-alpha stimulation of human lung fibroblasts.. Proteomics, [PMID:18546154] |
| 6. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 7. Rice, Gillian I GI and 50 more authors.. (2009) Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response.. Nature genetics, [PMID:19525956] |
| 8. Ramantani, Georgia G and 25 more authors.. (2010) Expanding the phenotypic spectrum of lupus erythematosus in Aicardi-Goutières syndrome.. Arthritis and rheumatism, [PMID:20131292] |
| 9. Thiele, Holger H and 10 more authors.. (2010) Cerebral arterial stenoses and stroke: novel features of Aicardi-Goutières syndrome caused by the Arg164X mutation in SAMHD1 are associated with altered cytokine expression.. Human mutation, [PMID:20842748] |
| 10. Ravenscroft, Jane C JC, Suri, Mohnish M, Rice, Gillian I GI, Szynkiewicz, Marcin M and Crow, Yanick J YJ.. (2011) Autosomal dominant inheritance of a heterozygous mutation in SAMHD1 causing familial chilblain lupus.. American journal of medical genetics. Part A, [PMID:21204240] |
| 11. Laguette, Nadine N and 9 more authors.. (2011) SAMHD1 is the dendritic- and myeloid-cell-specific HIV-1 restriction factor counteracted by Vpx.. Nature, (25): [PMID:21613998] |
| 12. Hrecka, Kasia K and 8 more authors.. (2011) Vpx relieves inhibition of HIV-1 infection of macrophages mediated by the SAMHD1 protein.. Nature, (29): [PMID:21720370] |
| 13. Goldstone, David C DC and 14 more authors.. (2011) HIV-1 restriction factor SAMHD1 is a deoxynucleoside triphosphate triphosphohydrolase.. Nature, (6): [PMID:22056990] |
| 14. Welbourn, Sarah S, Miyagi, Eri E, White, Tommy E TE, Diaz-Griffero, Felipe F and Strebel, Klaus K.. (2012) Identification and characterization of naturally occurring splice variants of SAMHD1.. Retrovirology, (23): [PMID:23092512] |
| 15. Beloglazova, Natalia N and 6 more authors.. (2013) Nuclease activity of the human SAMHD1 protein implicated in the Aicardi-Goutieres syndrome and HIV-1 restriction.. The Journal of biological chemistry, (22): [PMID:23364794] |
| 16. White, Tommy E TE and 7 more authors.. (2013) The retroviral restriction ability of SAMHD1, but not its deoxynucleotide triphosphohydrolase activity, is regulated by phosphorylation.. Cell host & microbe, (17): [PMID:23601106] |
| 17. Cribier, Alexandra A, Descours, Benjamin B, Valadão, Ana Luiza Chaves AL, Laguette, Nadine N and Benkirane, Monsef M.. (2013) Phosphorylation of SAMHD1 by cyclin A2/CDK1 regulates its restriction activity toward HIV-1.. Cell reports, (25): [PMID:23602554] |
| 18. Franzolin, Elisa E and 7 more authors.. (2013) The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells.. Proceedings of the National Academy of Sciences of the United States of America, (27): [PMID:23858451] |
| 19. Zhao, Ke K and 13 more authors.. (2013) Modulation of LINE-1 and Alu/SVA retrotransposition by Aicardi-Goutières syndrome-related SAMHD1.. Cell reports, (26): [PMID:24035396] |
| 20. Ji, Xiaoyun X and 10 more authors.. (2013) Mechanism of allosteric activation of SAMHD1 by dGTP.. Nature structural & molecular biology, [PMID:24141705] |
| 21. Rice, Gillian I GI and 55 more authors.. (2013) Assessment of interferon-related biomarkers in Aicardi-Goutières syndrome associated with mutations in TREX1, RNASEH2A, RNASEH2B, RNASEH2C, SAMHD1, and ADAR: a case-control study.. The Lancet. Neurology, [PMID:24183309] |
| 22. Zhu, Chunfeng C and 12 more authors.. (2013) Structural insight into dGTP-dependent activation of tetrameric SAMHD1 deoxynucleoside triphosphate triphosphohydrolase.. Nature communications, [PMID:24217394] |
| 23. Schwefel, David D and 7 more authors.. (2014) Structural basis of lentiviral subversion of a cellular protein degradation pathway.. Nature, (9): [PMID:24336198] |
| 24. Ryoo, Jeongmin J and 15 more authors.. (2014) The ribonuclease activity of SAMHD1 is required for HIV-1 restriction.. Nature medicine, [PMID:25038827] |
| 25. Ji, Xiaoyun X, Tang, Chenxiang C, Zhao, Qi Q, Wang, Wei W and Xiong, Yong Y.. (2014) Structural basis of cellular dNTP regulation by SAMHD1.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:25267621] |
| 26. Koharudin, Leonardus M I LM and 5 more authors.. (2014) Structural basis of allosteric activation of sterile α motif and histidine-aspartate domain-containing protein 1 (SAMHD1) by nucleoside triphosphates.. The Journal of biological chemistry, (21): [PMID:25288794] |
| 27. Zhu, Chun Feng CF and 5 more authors.. (2015) The mechanism of substrate-controlled allosteric regulation of SAMHD1 activated by GTP.. Acta crystallographica. Section D, Biological crystallography, [PMID:25760601] |
| 28. Seamon, Kyle J; Sun, Zhiqiang; Shlyakhtenko, Luda S; Lyubchenko, Yuri L and Stivers, James T.. (2015) SAMHD1 is a single-stranded nucleic acid binding protein with no active site-associated nuclease activity.. Nucleic acids research, (27): [PMID:26101257] |
| 29. Tang, Chenxiang C, Ji, Xiaoyun X, Wu, Li L and Xiong, Yong Y.. (2015) Impaired dNTPase activity of SAMHD1 by phosphomimetic mutation of Thr-592.. The Journal of biological chemistry, (30): [PMID:26294762] |
| 30. Arnold, Laurence H LH and 14 more authors.. (2015) Phospho-dependent Regulation of SAMHD1 Oligomerisation Couples Catalysis and Restriction.. PLoS pathogens, [PMID:26431200] |
| 31. Maelfait, Jonathan J, Bridgeman, Anne A, Benlahrech, Adel A, Cursi, Chiara C and Rehwinkel, Jan J.. (2016) Restriction by SAMHD1 Limits cGAS/STING-Dependent Innate and Adaptive Immune Responses to HIV-1.. Cell reports, (9): [PMID:27477283] |
| 32. White, Tommy E TE and 7 more authors.. (2017) A SAMHD1 mutation associated with Aicardi-Goutières syndrome uncouples the ability of SAMHD1 to restrict HIV-1 from its ability to downmodulate type I interferon in humans.. Human mutation, [PMID:28229507] |
| 33. Daddacha, Waaqo W and 27 more authors.. (2017) SAMHD1 Promotes DNA End Resection to Facilitate DNA Repair by Homologous Recombination.. Cell reports, (22): [PMID:28834754] |
| 34. Rocha-Perugini, Vera V and 10 more authors.. (2017) CD81 association with SAMHD1 enhances HIV-1 reverse transcription by increasing dNTP levels.. Nature microbiology, [PMID:28871089] |
| 35. Buzovetsky, Olga O and 6 more authors.. (2018) The SAM domain of mouse SAMHD1 is critical for its activation and regulation.. Nature communications, (29): [PMID:29379009] |
| 36. Herrmann, Alexandra A and 6 more authors.. (2018) The SAMHD1-mediated block of LINE-1 retroelements is regulated by phosphorylation.. Mobile DNA, [PMID:29610582] |
| 37. Coquel, Flavie F and 20 more authors.. (2018) SAMHD1 acts at stalled replication forks to prevent interferon induction.. Nature, [PMID:29670289] |
