| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab126421-10μl | 10μl | In stock | $59.90 | |
| Ab126421-50μl | 50μl | In stock | $179.90 | |
| Ab126421-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $279.90 | |
| Ab126421-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human SAMHD1 Antibody; WB; Unconjugated
| Product Name | Recombinant SAMHD1 Antibody - Primary antibody, specific to SAMHD1, Rabbit IgG |
|---|---|
| Synonyms | CHBL2 antibody | DCIP antibody | Dendritic cell derived IFNG induced protein antibody | Dendritic cell-derived IFNG-induced protein antibody | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody | dNTPase antibody | HD domain containing 1 ant |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.33 mg/mL |
| Host species | Rabbit |
| Specificity | SAMHD1 |
| Immunogen | A synthetic peptide derived from human SAMHD1 (AA 580-626) |
| Positive Control | WB: MCF7, K562, Ramos, Hep G2 and HeLa cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human SAMHD1 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.33 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant SAMHD1 Antibody (Ab126421) - Western Blot
All lanes: Recombinant SAMHD1 Antibody (Ab126421) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 70 kDa
Exposure time: 7.6 s
Recombinant SAMHD1 Antibody (Ab126421) - Western Blot
All lanes: Recombinant SAMHD1 Antibody (Ab126421) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 70 kDa
Exposure time: 10.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0405047 | Certificate of Analysis | Apr 30, 2024 | Ab126421 |
| ZJ24F0405046 | Certificate of Analysis | Apr 30, 2024 | Ab126421 |
| 1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052] [10.1021/op500134e] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Li, N N, Zhang, W W and Cao, X X.. (2000) Identification of human homologue of mouse IFN-gamma induced protein from human dendritic cells.. Immunology letters, (1): [PMID:11064105] |
| 4. Wiemann, S S and 23 more authors.. (2001) Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.. Genome research, [PMID:11230166] |
| 5. Liao, Wupeng W, Bao, Zhang Z, Cheng, Chang C, Mok, Yu-Keung YK and Wong, W S Fred WS.. (2008) Dendritic cell-derived interferon-gamma-induced protein mediates tumor necrosis factor-alpha stimulation of human lung fibroblasts.. Proteomics, [PMID:18546154] |
| 6. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 7. Rice, Gillian I GI and 50 more authors.. (2009) Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response.. Nature genetics, [PMID:19525956] |
| 8. Ramantani, Georgia G and 25 more authors.. (2010) Expanding the phenotypic spectrum of lupus erythematosus in Aicardi-Goutières syndrome.. Arthritis and rheumatism, [PMID:20131292] |
| 9. Thiele, Holger H and 10 more authors.. (2010) Cerebral arterial stenoses and stroke: novel features of Aicardi-Goutières syndrome caused by the Arg164X mutation in SAMHD1 are associated with altered cytokine expression.. Human mutation, [PMID:20842748] |
| 10. Ravenscroft, Jane C JC, Suri, Mohnish M, Rice, Gillian I GI, Szynkiewicz, Marcin M and Crow, Yanick J YJ.. (2011) Autosomal dominant inheritance of a heterozygous mutation in SAMHD1 causing familial chilblain lupus.. American journal of medical genetics. Part A, [PMID:21204240] |
| 11. Laguette, Nadine N and 9 more authors.. (2011) SAMHD1 is the dendritic- and myeloid-cell-specific HIV-1 restriction factor counteracted by Vpx.. Nature, (25): [PMID:21613998] |
| 12. Hrecka, Kasia K and 8 more authors.. (2011) Vpx relieves inhibition of HIV-1 infection of macrophages mediated by the SAMHD1 protein.. Nature, (29): [PMID:21720370] |
| 13. Goldstone, David C DC and 14 more authors.. (2011) HIV-1 restriction factor SAMHD1 is a deoxynucleoside triphosphate triphosphohydrolase.. Nature, (6): [PMID:22056990] |
| 14. Welbourn, Sarah S, Miyagi, Eri E, White, Tommy E TE, Diaz-Griffero, Felipe F and Strebel, Klaus K.. (2012) Identification and characterization of naturally occurring splice variants of SAMHD1.. Retrovirology, (23): [PMID:23092512] |
| 15. Beloglazova, Natalia N and 6 more authors.. (2013) Nuclease activity of the human SAMHD1 protein implicated in the Aicardi-Goutieres syndrome and HIV-1 restriction.. The Journal of biological chemistry, (22): [PMID:23364794] |
| 16. White, Tommy E TE and 7 more authors.. (2013) The retroviral restriction ability of SAMHD1, but not its deoxynucleotide triphosphohydrolase activity, is regulated by phosphorylation.. Cell host & microbe, (17): [PMID:23601106] |
| 17. Cribier, Alexandra A, Descours, Benjamin B, Valadão, Ana Luiza Chaves AL, Laguette, Nadine N and Benkirane, Monsef M.. (2013) Phosphorylation of SAMHD1 by cyclin A2/CDK1 regulates its restriction activity toward HIV-1.. Cell reports, (25): [PMID:23602554] |
| 18. Franzolin, Elisa E and 7 more authors.. (2013) The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells.. Proceedings of the National Academy of Sciences of the United States of America, (27): [PMID:23858451] |
| 19. Zhao, Ke K and 13 more authors.. (2013) Modulation of LINE-1 and Alu/SVA retrotransposition by Aicardi-Goutières syndrome-related SAMHD1.. Cell reports, (26): [PMID:24035396] |
| 20. Ji, Xiaoyun X and 10 more authors.. (2013) Mechanism of allosteric activation of SAMHD1 by dGTP.. Nature structural & molecular biology, [PMID:24141705] |
| 21. Rice, Gillian I GI and 55 more authors.. (2013) Assessment of interferon-related biomarkers in Aicardi-Goutières syndrome associated with mutations in TREX1, RNASEH2A, RNASEH2B, RNASEH2C, SAMHD1, and ADAR: a case-control study.. The Lancet. Neurology, [PMID:24183309] |
| 22. Zhu, Chunfeng C and 12 more authors.. (2013) Structural insight into dGTP-dependent activation of tetrameric SAMHD1 deoxynucleoside triphosphate triphosphohydrolase.. Nature communications, [PMID:24217394] |
| 23. Schwefel, David D and 7 more authors.. (2014) Structural basis of lentiviral subversion of a cellular protein degradation pathway.. Nature, (9): [PMID:24336198] |
| 24. Ryoo, Jeongmin J and 15 more authors.. (2014) The ribonuclease activity of SAMHD1 is required for HIV-1 restriction.. Nature medicine, [PMID:25038827] |
| 25. Ji, Xiaoyun X, Tang, Chenxiang C, Zhao, Qi Q, Wang, Wei W and Xiong, Yong Y.. (2014) Structural basis of cellular dNTP regulation by SAMHD1.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:25267621] |
| 26. Koharudin, Leonardus M I LM and 5 more authors.. (2014) Structural basis of allosteric activation of sterile α motif and histidine-aspartate domain-containing protein 1 (SAMHD1) by nucleoside triphosphates.. The Journal of biological chemistry, (21): [PMID:25288794] |
| 27. Zhu, Chun Feng CF and 5 more authors.. (2015) The mechanism of substrate-controlled allosteric regulation of SAMHD1 activated by GTP.. Acta crystallographica. Section D, Biological crystallography, [PMID:25760601] |
| 28. Seamon, Kyle J; Sun, Zhiqiang; Shlyakhtenko, Luda S; Lyubchenko, Yuri L and Stivers, James T.. (2015) SAMHD1 is a single-stranded nucleic acid binding protein with no active site-associated nuclease activity.. Nucleic acids research, (27): [PMID:26101257] |
| 29. Tang, Chenxiang C, Ji, Xiaoyun X, Wu, Li L and Xiong, Yong Y.. (2015) Impaired dNTPase activity of SAMHD1 by phosphomimetic mutation of Thr-592.. The Journal of biological chemistry, (30): [PMID:26294762] |
| 30. Arnold, Laurence H LH and 14 more authors.. (2015) Phospho-dependent Regulation of SAMHD1 Oligomerisation Couples Catalysis and Restriction.. PLoS pathogens, [PMID:26431200] |
| 31. Maelfait, Jonathan J, Bridgeman, Anne A, Benlahrech, Adel A, Cursi, Chiara C and Rehwinkel, Jan J.. (2016) Restriction by SAMHD1 Limits cGAS/STING-Dependent Innate and Adaptive Immune Responses to HIV-1.. Cell reports, (9): [PMID:27477283] |
| 32. White, Tommy E TE and 7 more authors.. (2017) A SAMHD1 mutation associated with Aicardi-Goutières syndrome uncouples the ability of SAMHD1 to restrict HIV-1 from its ability to downmodulate type I interferon in humans.. Human mutation, [PMID:28229507] |
| 33. Daddacha, Waaqo W and 27 more authors.. (2017) SAMHD1 Promotes DNA End Resection to Facilitate DNA Repair by Homologous Recombination.. Cell reports, (22): [PMID:28834754] |
| 34. Rocha-Perugini, Vera V and 10 more authors.. (2017) CD81 association with SAMHD1 enhances HIV-1 reverse transcription by increasing dNTP levels.. Nature microbiology, [PMID:28871089] |
| 35. Buzovetsky, Olga O and 6 more authors.. (2018) The SAM domain of mouse SAMHD1 is critical for its activation and regulation.. Nature communications, (29): [PMID:29379009] |
| 36. Herrmann, Alexandra A and 6 more authors.. (2018) The SAMHD1-mediated block of LINE-1 retroelements is regulated by phosphorylation.. Mobile DNA, [PMID:29610582] |
| 37. Coquel, Flavie F and 20 more authors.. (2018) SAMHD1 acts at stalled replication forks to prevent interferon induction.. Nature, [PMID:29670289] |
