Recombinant SFT Antibody - Primary antibody, specific to UBE2D1, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human SFT Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IHC
  • WB
Item Number
Ab127323
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab127323-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab127323-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab127323-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab127323-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human SFT Antibody; WB, IHC; Unconjugated

Basic Description

Product NameRecombinant SFT Antibody - Primary antibody, specific to UBE2D1, Rabbit IgG
SynonymsE2(17)KB1 | SFT | Stimulator of Fe transport | UB2D1_HUMAN | UBC 4/5 | UBC4/5 | UBC4/5 homolog | UBCH 5 | UBCH 5A | UBCH5 | UBCH5A | Ube2d1 | Ubiquitin carrier protein | Ubiquitin carrier protein D1 | Ubiquitin conjugating enzyme E2 17 kDa 1 | Ubiquitin c
Specifications & PurityExactAb™, Validated, Recombinant, 0.4 mg/mL
Host speciesRabbit
SpecificityUBE2D1
ImmunogenA synthetic peptide derived from human SFT (AA 119-132)
Positive ControlWB: HeLa, Jurkat, A431, HEK293, RAW 264.7, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human SFT Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.4 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SFT Antibody (Ab127323) - Western Blot
All lanes: Recombinant SFT Antibody (Ab127323) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 16 kDa
Exposure time: 3.0 s

Recombinant SFT Antibody (Ab127323) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant SFT Antibody (Ab127323). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

UBE2D1 Tbio Ubiquitin-conjugating enzyme E2 D1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
UBE2D1 Tbio Ubiquitin-conjugating enzyme E2 D1 (1 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
IHC

1/50 - 1/100

WB

1/500-1/1000

Certificates

Certificate of Analysis(COA)

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Related Documents

Solution Calculators