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Recombinant SHP2 Antibody - Primary antibody, specific to PTPN11, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 1.0 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human SHP2 Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
    Application:
  • IHC
  • WB
Item Number
Ab127519
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab127519-10μl
10μl
In stock
$66.90
Ab127519-50μl
50μl
In stock
$189.90
Ab127519-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab127519-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human SHP2 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameRecombinant SHP2 Antibody - Primary antibody, specific to PTPN11, Rabbit IgG
SynonymsBPTP3 | CFC | JMML | METCDS | NS1 | PTP-1D | PTP2C | SH-PTP2 | SH-PTP3 | SHP2 | EC 3.1.3.48 | MGC14433 | protein tyrosine phosphatase, non-receptor type 11 | protein tyrosine phosphatase-2 | Protein-tyrosine phosphatase 1D | Protein-tyrosine phosphatase 2
Specifications & PurityExactAb™, Validated, Recombinant, 1.0 mg/mL
Host speciesRabbit
SpecificityPTPN11
ImmunogenA synthetic peptide derived from human SHP2 (AA 545-563)
Positive ControlWB: HeLa, HEK293, Jurkat, K562 and MCF7 cell lysates. IHC: Human breast cancer tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human SHP2 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function:
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SHP2 Antibody (Ab127519) - Western Blot
All lanes: Recombinant SHP2 Antibody (Ab127519) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 68 kDa
Observed band size: 67 kDa
Exposure time: 30.0 s

Recombinant SHP2 Antibody (Ab127519) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant SHP2 Antibody (Ab127519). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets

PTPN11 Tchem Tyrosine-protein phosphatase non-receptor type 11 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0403985Certificate of AnalysisApr 01, 2024 Ab127519
ZJ24F0403984Certificate of AnalysisApr 01, 2024 Ab127519

Related Documents

References

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71. Digilio, Maria Cristina MC and 7 more authors..  (2002)  Grouping of multiple-lentigines/LEOPARD and Noonan syndromes on the PTPN11 gene..  American journal of human genetics,      [PMID:12058348]
72. Kosaki, Kenjiro K and 9 more authors..  (2002)  PTPN11 (protein-tyrosine phosphatase, nonreceptor-type 11) mutations in seven Japanese patients with Noonan syndrome..  The Journal of clinical endocrinology and metabolism,      [PMID:12161469]
73. Maheshwari, M M and 10 more authors..  (2002)  PTPN11 mutations in Noonan syndrome type I: detection of recurrent mutations in exons 3 and 13..  Human mutation,      [PMID:12325025]
74. Schollen, Els E, Matthijs, Gert G, Gewillig, Marc M, Fryns, Jean-Pierre JP and Legius, Eric E..  (2003)  PTPN11 mutation in a large family with Noonan syndrome and dizygous twinning..  European journal of human genetics : EJHG,      [PMID:12529711]
75. Musante, Luciana L and 11 more authors..  (2003)  Spectrum of mutations in PTPN11 and genotype-phenotype correlation in 96 patients with Noonan syndrome and five patients with cardio-facio-cutaneous syndrome..  European journal of human genetics : EJHG,      [PMID:12634870]
76. Tartaglia, Marco M and 9 more authors..  (2003)  Somatic mutations in PTPN11 in juvenile myelomonocytic leukemia, myelodysplastic syndromes and acute myeloid leukemia..  Nature genetics,      [PMID:12717436]
77. Kondoh, Tatsuro T and 6 more authors..  (2003)  Noonan syndrome with leukaemoid reaction and overproduction of catecholamines: a case report..  European journal of pediatrics,      [PMID:12739139]
78. Sarkozy, A A and 10 more authors..  (2003)  Correlation between PTPN11 gene mutations and congenital heart defects in Noonan and LEOPARD syndromes..  Journal of medical genetics,      [PMID:12960218]
79. Kogata, Naoko N and 6 more authors..  (2003)  Identification of Fer tyrosine kinase localized on microtubules as a platelet endothelial cell adhesion molecule-1 phosphorylating kinase in vascular endothelial cells..  Molecular biology of the cell,      [PMID:12972546]
80. Ehrhardt, Gotz R A GR and 5 more authors..  (2003)  The inhibitory potential of Fc receptor homolog 4 on memory B cells..  Proceedings of the National Academy of Sciences of the United States of America,    (11):   [PMID:14597715]
81. Conti, E E and 6 more authors..  (2003)  A novel PTPN11 mutation in LEOPARD syndrome..  Human mutation,      [PMID:14961557]
82. Wang, Jian Feng JF, Zhang, Xuefeng X and Groopman, Jerome E JE..  (2004)  Activation of vascular endothelial growth factor receptor-3 and its downstream signaling promote cell survival under oxidative stress..  The Journal of biological chemistry,    (25):   [PMID:15102829]
83. Sarkozy, A A and 9 more authors..  (2004)  Clinical and molecular analysis of 30 patients with multiple lentigines LEOPARD syndrome..  Journal of medical genetics,      [PMID:15121796]
84. Bertola, Débora Romeo DR, Pereira, Alexandre C AC, de Oliveira, Paulo S L PS, Kim, Chong A CA and Krieger, José Eduardo JE..  (2004)  Clinical variability in a Noonan syndrome family with a new PTPN11 gene mutation..  American journal of medical genetics. Part A,    (1):   [PMID:15384080]
85. Yoshida, Rie R and 5 more authors..  (2004)  Two novel and one recurrent PTPN11 mutations in LEOPARD syndrome..  American journal of medical genetics. Part A,    (1):   [PMID:15389709]
86. Keren, B B and 17 more authors..  (2004)  PTPN11 mutations in patients with LEOPARD syndrome: a French multicentric experience..  Journal of medical genetics,      [PMID:15520399]
87. Rönnstrand, L L..  (2004)  Signal transduction via the stem cell factor receptor/c-Kit..  Cellular and molecular life sciences : CMLS,      [PMID:15526160]
88. Rush, John J and 9 more authors..  (2005)  Immunoaffinity profiling of tyrosine phosphorylation in cancer cells..  Nature biotechnology,      [PMID:15592455]
89. Kalidas, Kamini K and 9 more authors..  (2005)  Genetic heterogeneity in LEOPARD syndrome: two families with no mutations in PTPN11..  Journal of human genetics,      [PMID:15690106]
90. Takahashi, Kunihiko K and 9 more authors..  (2005)  A novel mutation in the PTPN11 gene in a patient with Noonan syndrome and rapidly progressive hypertrophic cardiomyopathy..  European journal of pediatrics,      [PMID:15889278]
91. Bertola, Debora R DR and 7 more authors..  (2005)  Neurofibromatosis-Noonan syndrome: molecular evidence of the concurrence of both disorders in a patient..  American journal of medical genetics. Part A,    (30):   [PMID:15948193]
92. Uçar, Canan C, Calýskan, Umran U, Martini, Susanne S and Heinritz, Wolfram W..  (2006)  Acute myelomonocytic leukemia in a boy with LEOPARD syndrome (PTPN11 gene mutation positive)..  Journal of pediatric hematology/oncology,      [PMID:16679933]
93. Digilio, M Cristina MC and 5 more authors..  (2006)  PTPN11 gene mutations: linking the Gln510Glu mutation to the "LEOPARD syndrome phenotype"..  European journal of pediatrics,      [PMID:16733669]
94. Charest, Al A and 11 more authors..  (2006)  ROS fusion tyrosine kinase activates a SH2 domain-containing phosphatase-2/phosphatidylinositol 3-kinase/mammalian target of rapamycin signaling axis to form glioblastoma in mice..  Cancer research,    (1):   [PMID:16885344]
95. Traina, Fabíola F and 6 more authors..  (2006)  ANKHD1, ankyrin repeat and KH domain containing 1, is overexpressed in acute leukemias and is associated with SHP2 in K562 cells..  Biochimica et biophysica acta,      [PMID:16956752]
96. Wilson, Timothy J TJ and 5 more authors..  (2007)  FcRL6, a new ITIM-bearing receptor on cytolytic cells, is broadly expressed by lymphocytes following HIV-1 infection..  Blood,    (1):   [PMID:17213291]
97. Hoffmann, Sabrina C SC and 7 more authors..  (2007)  Identification of CLEC12B, an inhibitory receptor on myeloid cells..  The Journal of biological chemistry,    (3):   [PMID:17562706]
98. Lee, Hsiao-Hui HH and Chang, Zee-Fen ZF..  (2008)  Regulation of RhoA-dependent ROCKII activation by Shp2..  The Journal of cell biology,    (16):   [PMID:18559669]
99. Yu, Ming-Can MC and 14 more authors..  (2008)  An essential function for beta-arrestin 2 in the inhibitory signaling of natural killer cells..  Nature immunology,      [PMID:18604210]
100. Jakob, Sascha S and 6 more authors..  (2008)  Nuclear protein tyrosine phosphatase Shp-2 is one important negative regulator of nuclear export of telomerase reverse transcriptase..  The Journal of biological chemistry,    (28):   [PMID:18829466]
101. Ko, Jung Min JM, Kim, Jae-Min JM, Kim, Gu-Hwan GH and Yoo, Han-Wook HW..  (2008)  PTPN11, SOS1, KRAS, and RAF1 gene analysis, and genotype-phenotype correlation in Korean patients with Noonan syndrome..  Journal of human genetics,      [PMID:19020799]
102. Barr, Alastair J AJ and 9 more authors..  (2009)  Large-scale structural analysis of the classical human protein tyrosine phosphatome..  Cell,    (23):   [PMID:19167335]
103. Yo, Koji K and 9 more authors..  (2009)  SHP-2 inhibits tyrosine phosphorylation of Cas-L and regulates cell migration..  Biochemical and biophysical research communications,    (24):   [PMID:19275884]
104. Dasgupta, Bidisha B, Dufour, Eric E, Mamdouh, Zahra Z and Muller, William A WA..  (2009)  A novel and critical role for tyrosine 663 in platelet endothelial cell adhesion molecule-1 trafficking and transendothelial migration..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:19342684]
105. Tashiro, Kyoko K and 7 more authors..  (2009)  GAREM, a novel adaptor protein for growth factor receptor-bound protein 2, contributes to cellular transformation through the activation of extracellular signal-regulated kinase signaling..  The Journal of biological chemistry,    (24):   [PMID:19509291]
106. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
107. Kochi, Yuta Y and 6 more authors..  (2009)  FCRL3, an autoimmune susceptibility gene, has inhibitory potential on B-cell receptor-mediated signaling..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:19843936]
108. Sobreira, Nara L M NL and 15 more authors..  (2010)  Whole-genome sequencing of a single proband together with linkage analysis identifies a Mendelian disease gene..  PLoS genetics,    (17):   [PMID:20577567]
109. Mazharian, Alexandra A and 14 more authors..  (2012)  Mice lacking the ITIM-containing receptor G6b-B exhibit macrothrombocytopenia and aberrant platelet function..  Science signaling,    (30):   [PMID:23112346]
110. Edwards, Jonathan J JJ and 8 more authors..  (2014)  A PTPN11 allele encoding a catalytically impaired SHP2 protein in a patient with a Noonan syndrome phenotype..  American journal of medical genetics. Part A,      [PMID:24891296]
111. Noda, Saori S, Takahashi, Atsushi A, Hayashi, Takeru T, Tanuma, Sei-ichi S and Hatakeyama, Masanori M..  (2016)  Determination of the catalytic activity of LEOPARD syndrome-associated SHP2 mutants toward parafibromin, a bona fide SHP2 substrate involved in Wnt signaling..  Biochemical and biophysical research communications,    (22):   [PMID:26742426]
112. Pannone, Luca L and 28 more authors..  (2017)  Structural, Functional, and Clinical Characterization of a Novel PTPN11 Mutation Cluster Underlying Noonan Syndrome..  Human mutation,      [PMID:28074573]
113. Bongard, Robert D RD and 10 more authors..  (2017)  Serendipitous discovery of light-induced (In Situ) formation of an Azo-bridged dimeric sulfonated naphthol as a potent PTP1B inhibitor..  BMC biochemistry,    (31):   [PMID:28569147]

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