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Recombinant SIRP alpha Antibody - Primary antibody, specific to SIRPA, Rabbit IgG

Recombinant

Validated

ExactAb™

0.7 mg/mL

Features and benefits

Short Overview:
Recombinant; Rabbit anti Human SIRP alpha Antibody; WB, ICC, IF; Unconjugated
Species reactivity(Reacts with): Human
Isotype: Rabbit IgG

Application:

IF/ICC
WB

Item Number

Ab127634

Grouped product items
SKU	Size	Availability	Price
Ab127634-10μl	10μl	In stock	$69.90
Ab127634-50μl	50μl	In stock	$189.90
Ab127634-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab127634-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,699.90

Recombinant; Rabbit anti Human SIRP alpha Antibody; WB, ICC, IF; Unconjugated

View related series

Click to view target information: SIRPA

Accession#:P78324 Gene ID:140885 SIRP alpha SIRPA

Basic Description

Product Name	Recombinant SIRP alpha Antibody - Primary antibody, specific to SIRPA, Rabbit IgG
Synonyms	Signal regulatory protein alpha type 1 antibody \| Bit antibody \| Brain Ig like molecule with tyrosine based activation motifs antibody \| Brain Ig-like molecule with tyrosine-based activation motifs antibody \| Brain immunoglobulin like molecule with tyrosi
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.7 mg/mL
Host species	Rabbit
Specificity	SIRPA
Immunogen	Recombinant protein of human SIRP alpha (AA 31-373)
Positive Control	WB: HEK293, A549, U937 and THP-1 cell lysates. ICC/IF: HeLa cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human SIRP alpha Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.7 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SIRP alpha Antibody (Ab127634) - Western Blot
All lanes: Recombinant SIRP alpha Antibody (Ab127634) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 55 kDa
Observed band size: 74 kDa
Exposure time: 65.4 s

Recombinant SIRP alpha Antibody (Ab127634) - IF
Immunocytochemistry analysis of SIRP alpha (green) in HeLa using Recombinant SIRP alpha Antibody (Ab127634), and DAPI (blue).

Application

Dilution info

1/500-1/1000

ICC/IF

1/50-1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0403986	Certificate of Analysis	Apr 01, 2024	Ab127634
ZJ24F0403987	Certificate of Analysis	Apr 01, 2024	Ab127634

References

1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052]
2. Kharitonenkov, A A and 5 more authors.. (1997) A family of proteins that inhibit signalling through tyrosine kinase receptors.. Nature, (13): [PMID:9062191]
3. Yamao, T T and 7 more authors.. (1997) Mouse and human SHPS-1: molecular cloning of cDNAs and chromosomal localization of genes.. Biochemical and biophysical research communications, (3): [PMID:9070220]
4. Brooke, G P GP, Parsons, K R KR and Howard, C J CJ.. (1998) Cloning of two members of the SIRP alpha family of protein tyrosine phosphatase binding proteins in cattle that are expressed on monocytes and a subpopulation of dendritic cells and which mediate binding to CD4 T cells.. European journal of immunology, [PMID:9485180]
5. Timms, J F JF and 6 more authors.. (1999) SHPS-1 is a scaffold for assembling distinct adhesion-regulated multi-protein complexes in macrophages.. Current biology : CB, (26): [PMID:10469599]
6. Sano, S S, Ohnishi, H H and Kubota, M M.. (1999) Gene structure of mouse BIT/SHPS-1.. The Biochemical journal, (15): [PMID:10585853]
7. Stofega, M R MR, Argetsinger, L S LS, Wang, H H, Ullrich, A A and Carter-Su, C C.. (2000) Negative regulation of growth hormone receptor/JAK2 signaling by signal regulatory protein alpha.. The Journal of biological chemistry, (8): [PMID:10842184]
8. Latour, S S and 11 more authors.. (2001) Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:11509594]
9. Liu, Tao T and 6 more authors.. () Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952]
10. Hatherley, Deborah D and 5 more authors.. (2008) Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47.. Molecular cell, (25): [PMID:18657508]
11. Yao, Mingyi M and 10 more authors.. (2014) Thrombospondin-1 activation of signal-regulatory protein-α stimulates reactive oxygen species production and promotes renal ischemia reperfusion injury.. Journal of the American Society of Nephrology : JASN, [PMID:24511121]

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