| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab127634-10μl | 10μl | In stock | $69.90 | |
| Ab127634-50μl | 50μl | In stock | $189.90 | |
| Ab127634-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab127634-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human SIRP alpha Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant SIRP alpha Antibody - Primary antibody, specific to SIRPA, Rabbit IgG |
|---|---|
| Synonyms | Signal regulatory protein alpha type 1 antibody | Bit antibody | Brain Ig like molecule with tyrosine based activation motifs antibody | Brain Ig-like molecule with tyrosine-based activation motifs antibody | Brain immunoglobulin like molecule with tyrosi |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.7 mg/mL |
| Host species | Rabbit |
| Specificity | SIRPA |
| Immunogen | Recombinant protein of human SIRP alpha (AA 31-373) |
| Positive Control | WB: HEK293, A549, U937 and THP-1 cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human SIRP alpha Antibody, Recombinant, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant SIRP alpha Antibody (Ab127634) - Western Blot
All lanes: Recombinant SIRP alpha Antibody (Ab127634) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 74 kDa
Exposure time: 65.4 s
Recombinant SIRP alpha Antibody (Ab127634) - Western Blot
All lanes: Recombinant SIRP alpha Antibody (Ab127634) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 77 kDa
Exposure time: 30.0 s
Recombinant SIRP alpha Antibody (Ab127634) - IF
Immunocytochemistry analysis of SIRP alpha (green) in HeLa using Recombinant SIRP alpha Antibody (Ab127634), and DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0403986 | Certificate of Analysis | Apr 01, 2024 | Ab127634 |
| ZJ24F0403987 | Certificate of Analysis | Apr 01, 2024 | Ab127634 |
| 1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052] [10.1021/op500134e] |
| 2. Kharitonenkov, A A and 5 more authors.. (1997) A family of proteins that inhibit signalling through tyrosine kinase receptors.. Nature, (13): [PMID:9062191] |
| 3. Yamao, T T and 7 more authors.. (1997) Mouse and human SHPS-1: molecular cloning of cDNAs and chromosomal localization of genes.. Biochemical and biophysical research communications, (3): [PMID:9070220] |
| 4. Brooke, G P GP, Parsons, K R KR and Howard, C J CJ.. (1998) Cloning of two members of the SIRP alpha family of protein tyrosine phosphatase binding proteins in cattle that are expressed on monocytes and a subpopulation of dendritic cells and which mediate binding to CD4 T cells.. European journal of immunology, [PMID:9485180] |
| 5. Timms, J F JF and 6 more authors.. (1999) SHPS-1 is a scaffold for assembling distinct adhesion-regulated multi-protein complexes in macrophages.. Current biology : CB, (26): [PMID:10469599] |
| 6. Sano, S S, Ohnishi, H H and Kubota, M M.. (1999) Gene structure of mouse BIT/SHPS-1.. The Biochemical journal, (15): [PMID:10585853] |
| 7. Stofega, M R MR, Argetsinger, L S LS, Wang, H H, Ullrich, A A and Carter-Su, C C.. (2000) Negative regulation of growth hormone receptor/JAK2 signaling by signal regulatory protein alpha.. The Journal of biological chemistry, (8): [PMID:10842184] |
| 8. Latour, S S and 11 more authors.. (2001) Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:11509594] |
| 9. Liu, Tao T and 6 more authors.. () Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952] |
| 10. Hatherley, Deborah D and 5 more authors.. (2008) Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47.. Molecular cell, (25): [PMID:18657508] |
| 11. Yao, Mingyi M and 10 more authors.. (2014) Thrombospondin-1 activation of signal-regulatory protein-α stimulates reactive oxygen species production and promotes renal ischemia reperfusion injury.. Journal of the American Society of Nephrology : JASN, [PMID:24511121] |
