Recombinant SIRT2 Antibody - Primary antibody, specific to SIRT2, Rabbit IgG

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab127668
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab127668-10μl
10μl
In stock
$69.90
Ab127668-50μl
50μl
In stock
$189.90
Ab127668-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab127668-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human SIRT2 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameRecombinant SIRT2 Antibody - Primary antibody, specific to SIRT2, Rabbit IgG
SynonymsEC 2.3.1.286 | Regulatory protein SIR2 homolog 2 | SIR2-like protein 2 | FLJ35621 antibody | FLJ37491 antibody | NAD dependent deacetylase sirtuin 2 antibody | NAD dependent protein deacetylase sirtuin 2 antibody | NAD-dependent deacetylase sirtuin-2 anti
Specifications & PurityExactAb™, Validated, Recombinant, 0.5 mg/mL
Host speciesRabbit
SpecificitySIRT2
ImmunogenRecombinant protein of human SIRT2 (AA 2-389)
Positive ControlWB: HeLa and NIH/3T3 cell lysates. IHC: Human colon cancer tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human SIRT2 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/2000
IHC: 1/50-1/100
Protein Function
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SIRT2 Antibody (Ab127668) - Western Blot
All lanes: Recombinant SIRT2 Antibody (Ab127668) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 42 kDa
Exposure time: 15.7 s

Recombinant SIRT2 Antibody (Ab127668) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant SIRT2 Antibody (Ab127668). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

SIRT2 Tchem NAD-dependent protein deacetylase sirtuin-2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
SIRT2 Tchem NAD-dependent deacetylase sirtuin 2 (3979 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/2000

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1/500-1/2000

IHC1/50 - 1/100

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1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0403989Certificate of AnalysisApr 01, 2024 Ab127668
ZJ24F0403988Certificate of AnalysisApr 01, 2024 Ab127668

Related Documents

References

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65. Dryden, Sylvia C SC, Nahhas, Fatimah A FA, Nowak, James E JE, Goustin, Anton-Scott AS and Tainsky, Michael A MA..  (2003)  Role for human SIRT2 NAD-dependent deacetylase activity in control of mitotic exit in the cell cycle..  Molecular and cellular biology,      [PMID:12697818]
66. Hiratsuka, Masaharu M and 10 more authors..  (2003)  Proteomics-based identification of differentially expressed genes in human gliomas: down-regulation of SIRT2 gene..  Biochemical and biophysical research communications,    (26):   [PMID:12963026]
67. Bae, Nancy S NS, Swanson, Mark J MJ, Vassilev, Alex A and Howard, Bruce H BH..  (2004)  Human histone deacetylase SIRT2 interacts with the homeobox transcription factor HOXA10..  Journal of biochemistry,      [PMID:15213244]
68. Michishita, Eriko E, Park, Jean Y JY, Burneskis, Jenna M JM, Barrett, J Carl JC and Horikawa, Izumi I..  (2005)  Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins..  Molecular biology of the cell,      [PMID:16079181]
69. Heltweg, Birgit B and 10 more authors..  (2006)  Antitumor activity of a small-molecule inhibitor of human silent information regulator 2 enzymes..  Cancer research,    (15):   [PMID:16618762]
70. Vaquero, Alejandro A and 8 more authors..  (2006)  SirT2 is a histone deacetylase with preference for histone H4 Lys 16 during mitosis..  Genes & development,    (15):   [PMID:16648462]
71. Inoue, T T and 9 more authors..  (2007)  SIRT2, a tubulin deacetylase, acts to block the entry to chromosome condensation in response to mitotic stress..  Oncogene,    (15):   [PMID:16909107]
72. North, Brian J BJ and Verdin, Eric E..  (2007)  Mitotic regulation of SIRT2 by cyclin-dependent kinase 1-dependent phosphorylation..  The Journal of biological chemistry,    (6):   [PMID:17488717]
73. Nahhas, Fatimah F, Dryden, Sylvia C SC, Abrams, Judith J and Tainsky, Michael A MA..  (2007)  Mutations in SIRT2 deacetylase which regulate enzymatic activity but not its interaction with HDAC6 and tubulin..  Molecular and cellular biochemistry,      [PMID:17516032]
74. North, Brian J BJ and Verdin, Eric E..  (2007)  Interphase nucleo-cytoplasmic shuttling and localization of SIRT2 during mitosis..  PloS one,    (29):   [PMID:17726514]
75. Jin, Yun-Hye YH and 6 more authors..  (2008)  Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53..  Biochemical and biophysical research communications,    (11):   [PMID:18249187]
76. Lynn, Edward G; McLeod, Christopher J; Gordon, Jeffrey P; Bao, Jianjun and Sack, Michael N..  (2008)  SIRT2 is a negative regulator of anoxia-reoxygenation tolerance via regulation of 14-3-3 zeta and BAD in H9c2 cells..  FEBS letters,    (20):   [PMID:18640115]
77. Han, Younho Y and 7 more authors..  (2008)  Acetylation of Sirt2 by p300 attenuates its deacetylase activity..  Biochemical and biophysical research communications,    (31):   [PMID:18722353]
78. Black, Joshua C JC, Mosley, Amber A, Kitada, Tasuku T, Washburn, Michael M and Carey, Michael M..  (2008)  The SIRT2 deacetylase regulates autoacetylation of p300..  Molecular cell,    (7):   [PMID:18995842]
79. Inoue, Toshiaki T and 9 more authors..  (2009)  SIRT2 downregulation confers resistance to microtubule inhibitors by prolonging chronic mitotic arrest..  Cell cycle (Georgetown, Tex.),    (15):   [PMID:19282667]
80. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
81. Zhao, Ying Y and 9 more authors..  (2010)  Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity..  Nature cell biology,      [PMID:20543840]
82. Vempati, Rahul K RK and 5 more authors..  (2010)  p300-mediated acetylation of histone H3 lysine 56 functions in DNA damage response in mammals..  The Journal of biological chemistry,    (10):   [PMID:20587414]
83. Rothgiesser, Karin M KM, Erener, Süheda S, Waibel, Susanne S, Lüscher, Bernhard B and Hottiger, Michael O MO..  (2010)  SIRT2 regulates NF-κB dependent gene expression through deacetylation of p65 Lys310..  Journal of cell science,    (15):   [PMID:21081649]
84. Jiang, Wenqing W and 8 more authors..  (2011)  Acetylation regulates gluconeogenesis by promoting PEPCK1 degradation via recruiting the UBR5 ubiquitin ligase..  Molecular cell,    (8):   [PMID:21726808]
85. Maxwell, Michele M MM and 8 more authors..  (2011)  The Sirtuin 2 microtubule deacetylase is an abundant neuronal protein that accumulates in the aging CNS..  Human molecular genetics,    (15):   [PMID:21791548]
86. Beirowski, Bogdan B and 12 more authors..  (2011)  Sir-two-homolog 2 (Sirt2) modulates peripheral myelination through polarity protein Par-3/atypical protein kinase C (aPKC) signaling..  Proceedings of the National Academy of Sciences of the United States of America,    (25):   [PMID:21949390]
87. Kim, Hyun-Seok HS and 15 more authors..  (2011)  SIRT2 maintains genome integrity and suppresses tumorigenesis through regulating APC/C activity..  Cancer cell,    (18):   [PMID:22014574]
88. Ishfaq, Muhammad M and 5 more authors..  (2012)  Acetylation regulates subcellular localization of eukaryotic translation initiation factor 5A (eIF5A)..  FEBS letters,    (21):   [PMID:22771473]
89. Gal, Jiyeong J, Bang, Yeojin Y and Choi, Hyun Jin HJ..  (2012)  SIRT2 interferes with autophagy-mediated degradation of protein aggregates in neuronal cells under proteasome inhibition..  Neurochemistry international,      [PMID:22819792]
90. Park, Seong-Hoon SH and 7 more authors..  (2012)  SIRT2 is a tumor suppressor that connects aging, acetylome, cell cycle signaling, and carcinogenesis..  Translational cancer research,    (1):   [PMID:22943040]
91. Serrano, Lourdes L and 14 more authors..  (2013)  The tumor suppressor SirT2 regulates cell cycle progression and genome stability by modulating the mitotic deposition of H4K20 methylation..  Genes & development,    (15):   [PMID:23468428]
92. Choi, You Hee YH, Kim, Hangun H, Lee, Sung Ho SH, Jin, Yun-Hye YH and Lee, Kwang Youl KY..  (2013)  ERK1/2 regulates SIRT2 deacetylase activity..  Biochemical and biophysical research communications,    (26):   [PMID:23806683]
93. Lin, Ruiting R and 7 more authors..  (2013)  Acetylation stabilizes ATP-citrate lyase to promote lipid biosynthesis and tumor growth..  Molecular cell,    (22):   [PMID:23932781]
94. Rack, Johannes G M JG, VanLinden, Magali R MR, Lutter, Timo T, Aasland, Rein R and Ziegler, Mathias M..  (2014)  Constitutive nuclear localization of an alternatively spliced sirtuin-2 isoform..  Journal of molecular biology,    (17):   [PMID:24177535]
95. Hoffmann, Gesine G, Breitenbücher, Frank F, Schuler, Martin M and Ehrenhofer-Murray, Ann E AE..  (2014)  A novel sirtuin 2 (SIRT2) inhibitor with p53-dependent pro-apoptotic activity in non-small cell lung cancer..  The Journal of biological chemistry,    (21):   [PMID:24379401]
96. Yamagata, Kenichiro K and 10 more authors..  (2014)  Structural basis for potent inhibition of SIRT2 deacetylase by a macrocyclic peptide inducing dynamic structural change..  Structure (London, England : 1993),    (4):   [PMID:24389023]
97. Seo, K-S KS and 14 more authors..  (2015)  SIRT2 regulates tumour hypoxia response by promoting HIF-1α hydroxylation..  Oncogene,    (12):   [PMID:24681946]
98. Wang, Yi-Ping YP and 14 more authors..  (2014)  Regulation of G6PD acetylation by SIRT2 and KAT9 modulates NADPH homeostasis and cell survival during oxidative stress..  The EMBO journal,    (17):   [PMID:24769394]
99. Shi, Yi Y and 7 more authors..  (2014)  tRNA synthetase counteracts c-Myc to develop functional vasculature..  eLife,    (17):   [PMID:24940000]
100. Rumpf, Tobias T and 18 more authors..  (2015)  Selective Sirt2 inhibition by ligand-induced rearrangement of the active site..  Nature communications,    (12):   [PMID:25672491]
101. Teng, Yan-Bin YB and 7 more authors..  (2015)  Efficient demyristoylase activity of SIRT2 revealed by kinetic and structural studies..  Scientific reports,    (23):   [PMID:25704306]
102. Lee, Jin-Kwan JK and 9 more authors..  (2016)  Oncogenic microtubule hyperacetylation through BEX4-mediated sirtuin 2 inhibition..  Cell death & disease,    (11):   [PMID:27512957]
103. Jing, Hui H and 6 more authors..  (2017)  SIRT2 and lysine fatty acylation regulate the transforming activity of K-Ras4a..  eLife,    (14):   [PMID:29239724]

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