Basic Description

Product Name

Recombinant SIRT6 Antibody - Primary antibody, specific to SIRT6, Rabbit IgG

Synonyms

Specifications & Purity

ExactAb™, Validated, Carrier Free, Recombinant, 0.6 mg/mL

Host species

Rabbit

Specificity

SIRT6

Immunogen

A synthetic peptide derived from human SIRT6 (AA 19-33)

Positive Control

WB: K562, HeLa, MCF7, NIH/3T3 and C6 cell lysates.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Recombinant, Validated

Product Description

Rabbit anti Human SIRT6 Antibody, Recombinant, could be used for WB and so on.

Application:

WB: 1/1000

Protein Function：

NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	0.6 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SIRT6 Antibody (Ab127697) - Western Blot
All lanes: Recombinant SIRT6 Antibody (Ab127697) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 37, 41 kDa
Exposure time: 60.0 s

Recombinant SIRT6 Antibody (Ab127697) - Western Blot
All lanes: Recombinant SIRT6 Antibody (Ab127697) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 42 kDa
Exposure time: 20.0 s

Associated Targets

SIRT6 Tchem NAD-dependent protein deacetylase sirtuin-6 0 Activities

Discover Target: SIRT6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0405053	Certificate of Analysis	Apr 30, 2024	Ab127697
ZJ24F0405052	Certificate of Analysis	Apr 30, 2024	Ab127697

References

1. Huang Z, Zhao J, Deng W, Chen Y, Shang J, Song K, Zhang L, Wang C, Lu S, Yang X et al.. (2018) Identification of a cellularly active SIRT6 allosteric activator.. Nat Chem Biol, 14 (12): (1118-1126). [PMID:30374165]
2. Angst D, Gessier F, Janser P, Vulpetti A, Wälchli R, Beerli C, Littlewood-Evans A, Dawson J, Nuesslein-Hildesheim B, Wieczorek G et al.. (2020) Discovery of LOU064 (Remibrutinib), a Potent and Highly Selective Covalent Inhibitor of Bruton's Tyrosine Kinase.. J Med Chem, 63 (10): (5102-5118). [PMID:32083858]
3. Hong Jiang,Saba Khan,Yi Wang,Guillaume Charron,Bin He,Carlos Sebastian,Jintang Du,Ray Kim,Eva Ge,Raul Mostoslavsky,Howard C Hang,Quan Hao,Hening Lin. (2013-04-05) SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine.. Nature, 496 ((7443)): (110-113). [PMID:23552949]
4. Beatriz E Bolívar,John T Welch. (2017-02-22) Studies of the Binding of Modest Modulators of the Human Enzyme, Sirtuin\u20056, by STD NMR.. Chembiochem : a European journal of chemical biology, 18 ((10)): (931-940). [PMID:28222243]
5. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
6. Parenti MD, Grozio A, Bauer I, Galeno L, Damonte P, Millo E, Sociali G, Franceschi C, Ballestrero A, Bruzzone S, Del Rio A, Nencioni A.. (2014) Discovery of novel and selective SIRT6 inhibitors.. J Med Chem, 57 (11): (4796-4804). [PMID:24785705]
7. Zang W, Hao Y, Wang Z, Zheng W.. (2015) Novel thiourea-based sirtuin inhibitory warheads.. Bioorg Med Chem Lett, 25 (16): (3319-3324). [PMID:26081291]
8. Sociali G, Galeno L, Parenti MD, Grozio A, Bauer I, Passalacqua M, Boero S, Donadini A, Millo E, Bellotti M, Sturla L, Damonte P, Puddu A, Ferroni C, Varchi G, Franceschi C, Ballestrero A, Poggi A, Bruzzone S, Nencioni A, Del Rio A.. (2015) Quinazolinedione SIRT6 inhibitors sensitize cancer cells to chemotherapeutics.. Eur J Med Chem, 102 (530-539). [PMID:26310895]
9. Chen D, Zheng W.. (2016) Cyclic peptide-based potent and selective SIRT1/2 dual inhibitors harboring Nε-thioacetyl-lysine.. Bioorg Med Chem Lett, 26 (21): (5234-5239). [PMID:27707605]
10. Yang LL, Wang HL, Zhong L, Yuan C, Liu SY, Yu ZJ, Liu S, Yan YH, Wu C, Wang Y, Wang Z, Yu Y, Chen Q, Li GB.. (2018) X-ray crystal structure guided discovery of new selective, substrate-mimicking sirtuin 2 inhibitors that exhibit activities against non-small cell lung cancer cells.. Eur J Med Chem, 155 (806-823). [PMID:29957526]
11. Damonte P, Sociali G, Parenti MD, Soncini D, Bauer I, Boero S, Grozio A, Holtey MV, Piacente F, Becherini P, Sanguineti R, Salis A, Damonte G, Cea M, Murone M, Poggi A, Nencioni A, Del Rio A, Bruzzone S.. (2017) SIRT6 inhibitors with salicylate-like structure show immunosuppressive and chemosensitizing effects.. Bioorg Med Chem, 25 (20): (5849-5858). [PMID:28958848]
12. Li Y, Zhou Y, Wang F, Chen X, Wang C, Wang J, Liu T, Li Y, He B.. (2018) SIRT4 is the last puzzle of mitochondrial sirtuins.. Bioorg Med Chem, 26 (14): (3861-3865). [PMID:30033389]
13. Bouchet S, Linot C, Ruzic D, Agbaba D, Fouchaq B, Roche J, Nikolic K, Blanquart C, Bertrand P.. (2019) Extending Cross Metathesis To Identify Selective HDAC Inhibitors: Synthesis, Biological Activities, and Modeling.. ACS Med Chem Lett, 10 (6): (863-868). [PMID:31223439]
14. Sun W, Chen X, Huang S, Li W, Tian C, Yang S, Li L.. (2020) Discovery of 5-(4-methylpiperazin-1-yl)-2-nitroaniline derivatives as a new class of SIRT6 inhibitors.. Bioorg Med Chem Lett, 30 (16): (127215-127215). [PMID:32631504]
15. Chen X, Sun W, Huang S, Zhang H, Lin G, Li H, Qiao J, Li L, Yang S.. (2020) Discovery of Potent Small-Molecule SIRT6 Activators: Structure-Activity Relationship and Anti-Pancreatic Ductal Adenocarcinoma Activity.. J Med Chem, 63 (18): (10474-10495). [PMID:32787077]
16. Yang LL,Wang HL,Yan YH,Liu S,Yu ZJ,Huang MY,Luo Y,Zheng X,Yu Y,Li GB. (2020) Sensitive fluorogenic substrates for sirtuin deacylase inhibitor discovery.. Eur J Med Chem, 192 (112201-112201). [PMID:32163813]
17. and Frye, R A RA.. (2000) Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins.. Biochemical and biophysical research communications, (5): [PMID:10873683]
18. Michishita, Eriko E, Park, Jean Y JY, Burneskis, Jenna M JM, Barrett, J Carl JC and Horikawa, Izumi I.. (2005) Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins.. Molecular biology of the cell, [PMID:16079181]
19. Michishita, Eriko E and 14 more authors.. (2008) SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin.. Nature, (27): [PMID:18337721]
20. Kawahara, Tiara L A TL and 10 more authors.. (2009) SIRT6 links histone H3 lysine 9 deacetylation to NF-kappaB-dependent gene expression and organismal life span.. Cell, (9): [PMID:19135889]
21. Michishita, Eriko E and 6 more authors.. (2009) Cell cycle-dependent deacetylation of telomeric histone H3 lysine K56 by human SIRT6.. Cell cycle (Georgetown, Tex.), (15): [PMID:19625767]
22. Kaidi, Abderrahmane A, Weinert, Brian T BT, Choudhary, Chunaram C and Jackson, Stephen P SP.. (2010) Human SIRT6 promotes DNA end resection through CtIP deacetylation.. Science (New York, N.Y.), (10): [PMID:20829486]
23. Pan, Patricia W PW and 5 more authors.. (2011) Structure and biochemical functions of SIRT6.. The Journal of biological chemistry, (22): [PMID:21362626]
24. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
25. Mao, Zhiyong Z and 7 more authors.. (2011) SIRT6 promotes DNA repair under stress by activating PARP1.. Science (New York, N.Y.), (17): [PMID:21680843]
26. Tennen, Ruth I RI, Bua, Dennis J DJ, Wright, Woodring E WE and Chua, Katrin F KF.. (2011) SIRT6 is required for maintenance of telomere position effect in human cells.. Nature communications, (16): [PMID:21847107]
27. Mao, Zhiyong Z and 5 more authors.. (2012) Sirtuin 6 (SIRT6) rescues the decline of homologous recombination repair during replicative senescence.. Proceedings of the National Academy of Sciences of the United States of America, (17): [PMID:22753495]
28. Dominy, John E JE and 15 more authors.. (2012) The deacetylase Sirt6 activates the acetyltransferase GCN5 and suppresses hepatic gluconeogenesis.. Molecular cell, (28): [PMID:23142079]
29. Sebastián, Carlos C and 23 more authors.. (2012) The histone deacetylase SIRT6 is a tumor suppressor that controls cancer metabolism.. Cell, (7): [PMID:23217706]
30. Sharma, Amit A and 7 more authors.. (2013) The role of SIRT6 protein in aging and reprogramming of human induced pluripotent stem cells.. The Journal of biological chemistry, (21): [PMID:23653361]
31. Gil, Reuven; Barth, Shaul; Kanfi, Yariv and Cohen, Haim Y.. (2013) SIRT6 exhibits nucleosome-dependent deacetylase activity.. Nucleic acids research, [PMID:23892288]
32. Elhanati, Sivan S and 7 more authors.. (2013) Multiple regulatory layers of SREBP1/2 by SIRT6.. Cell reports, (12): [PMID:24012758]
33. Ronnebaum, Sarah M SM, Wu, Yaxu Y, McDonough, Holly H and Patterson, Cam C.. (2013) The ubiquitin ligase CHIP prevents SirT6 degradation through noncanonical ubiquitination.. Molecular and cellular biology, [PMID:24043303]
34. Feldman, Jessica L JL, Baeza, Josue J and Denu, John M JM.. (2013) Activation of the protein deacetylase SIRT6 by long-chain fatty acids and widespread deacylation by mammalian sirtuins.. The Journal of biological chemistry, (25): [PMID:24052263]
35. Zhang, Ping P and 16 more authors.. (2014) Tumor suppressor p53 cooperates with SIRT6 to regulate gluconeogenesis by promoting FoxO1 nuclear exclusion.. Proceedings of the National Academy of Sciences of the United States of America, (22): [PMID:25009184]
36. TenNapel, Mindi J MJ and 6 more authors.. (2014) SIRT6 minor allele genotype is associated with >5-year decrease in lifespan in an aged cohort.. PloS one, [PMID:25541994]
37. Etchegaray, Jean-Pierre and 17 more authors.. (2015) The histone deacetylase SIRT6 controls embryonic stem cell fate via TET-mediated production of 5-hydroxymethylcytosine.. Nature cell biology, [PMID:25915124]
38. Kugel, Sita S and 10 more authors.. (2015) Identification of and Molecular Basis for SIRT6 Loss-of-Function Point Mutations in Cancer.. Cell reports, (20): [PMID:26456828]
39. Elhanati, Sivan S and 7 more authors.. (2016) Reciprocal Regulation between SIRT6 and miR-122 Controls Liver Metabolism and Predicts Hepatocarcinoma Prognosis.. Cell reports, (12): [PMID:26748705]
40. Bhardwaj, Abhishek A and Das, Sanjeev S.. (2016) SIRT6 deacetylates PKM2 to suppress its nuclear localization and oncogenic functions.. Proceedings of the National Academy of Sciences of the United States of America, (2): [PMID:26787900]
41. Cai, J J and 7 more authors.. (2016) A crucial role of SUMOylation in modulating Sirt6 deacetylation of H3 at lysine 56 and its tumor suppressive activity.. Oncogene, (15): [PMID:26898756]
42. Tasselli, Luisa L and 7 more authors.. (2016) SIRT6 deacetylates H3K18ac at pericentric chromatin to prevent mitotic errors and cellular senescence.. Nature structural & molecular biology, [PMID:27043296]
43. Kugel, Sita S and 15 more authors.. (2016) SIRT6 Suppresses Pancreatic Cancer through Control of Lin28b.. Cell, (2): [PMID:27180906]
44. Zhang, Xiaoyu X and 8 more authors.. (2016) Identifying the functional contribution of the defatty-acylase activity of SIRT6.. Nature chemical biology, [PMID:27322069]
45. Van Meter, Michael M and 11 more authors.. (2016) JNK Phosphorylates SIRT6 to Stimulate DNA Double-Strand Break Repair in Response to Oxidative Stress by Recruiting PARP1 to DNA Breaks.. Cell reports, (6): [PMID:27568560]
46. Jain, Abhinav K and 10 more authors.. (2016) LncPRESS1 Is a p53-Regulated LncRNA that Safeguards Pluripotency by Disrupting SIRT6-Mediated De-acetylation of Histone H3K56.. Molecular cell, (1): [PMID:27912097]
47. Zhang, Xiaoyu; Spiegelman, Nicole A; Nelson, Ornella D; Jing, Hui and Lin, Hening.. (2017) SIRT6 regulates Ras-related protein R-Ras2 by lysine defatty-acylation.. eLife, (13): [PMID:28406396]
48. Ghosh, Shrestha S and 8 more authors.. (2018) Haploinsufficiency of Trp53 dramatically extends the lifespan of Sirt6-deficient mice.. eLife, (23): [PMID:29474172]
49. Ferrer, Christina M CM and 17 more authors.. (2018) An inactivating mutation in the histone deacetylase SIRT6 causes human perinatal lethality.. Genes & development, (1): [PMID:29555651]
50. Geng, Anke and 12 more authors.. (2020) The deacetylase SIRT6 promotes the repair of UV-induced DNA damage by targeting DDB2.. Nucleic acids research, (18): [PMID:32789493]

Solution Calculators

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Dilution Calculator

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SKU	Size	Availability	Price
Ab127697-10μl	10μl	In stock	$79.90
Ab127697-50μl	50μl	In stock	$239.90
Ab127697-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$399.90
Ab127697-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,999.90