| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab128163-10μl | 10μl | In stock | $66.90 | |
| Ab128163-50μl | 50μl | In stock | $199.90 | |
| Ab128163-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $319.90 | |
| Ab128163-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,809.90 |
Recombinant; Rabbit anti Human Smad1 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant Smad1 Antibody - Primary antibody, specific to SMAD1, Rabbit IgG |
|---|---|
| Synonyms | BSP-1 antibody | BSP1 antibody | HsMAD1 antibody | JV4-1 antibody | JV41 antibody | MAD homolog 1 antibody | MAD mothers against decapentaplegic homolog 1 antibody | Mad related protein 1 antibody | Mad-related protein 1 antibody | MADH1 antibody | MADR1 |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.8 mg/mL |
| Host species | Rabbit |
| Specificity | SMAD1 |
| Immunogen | A synthetic peptide derived from human Smad1 (AA 220-280). |
| Positive Control | WB: Hep G2, HEK293, HUVEC and HeLa cell lysates. IHC: Human colon tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Smad1 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Smad1 Antibody (Ab128163) - Western Blot
All lanes: Recombinant Smad1 Antibody (Ab128163) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 52 kDa
Observed band size: 57 kDa
Exposure time: 45.1 s
Recombinant Smad1 Antibody (Ab128163) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon using Recombinant Smad1 Antibody (Ab128163). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303519 | Certificate of Analysis | Mar 22, 2024 | Ab128163 |
| ZJ24F0303518 | Certificate of Analysis | Mar 22, 2024 | Ab128163 |
| 1. Liu, F F and 6 more authors.. (1996) A human Mad protein acting as a BMP-regulated transcriptional activator.. Nature, (13): [PMID:8637600] |
| 2. Hoodless, P A PA and 6 more authors.. (1996) MADR1, a MAD-related protein that functions in BMP2 signaling pathways.. Cell, (17): [PMID:8653785] |
| 3. Lechleider, R J RJ, de Caestecker, M P MP, Dehejia, A A, Polymeropoulos, M H MH and Roberts, A B AB.. (1996) Serine phosphorylation, chromosomal localization, and transforming growth factor-beta signal transduction by human bsp-1.. The Journal of biological chemistry, (26): [PMID:8663601] |
| 4. Riggins, G J GJ and 9 more authors.. (1996) Mad-related genes in the human.. Nature genetics, [PMID:8673135] |
| 5. Zhang, Y Y, Feng, X X, We, R R and Derynck, R R.. (1996) Receptor-associated Mad homologues synergize as effectors of the TGF-beta response.. Nature, (12): [PMID:8774881] |
| 6. Kretzschmar, M M, Liu, F F, Hata, A A, Doody, J J and Massagué, J J.. (1997) The TGF-beta family mediator Smad1 is phosphorylated directly and activated functionally by the BMP receptor kinase.. Genes & development, (15): [PMID:9136927] |
| 7. Massagué, J J.. (1998) TGF-beta signal transduction.. Annual review of biochemistry, [PMID:9759503] |
| 8. Verschueren, K K and Huylebroeck, D D.. () Remarkable versatility of Smad proteins in the nucleus of transforming growth factor-beta activated cells.. Cytokine & growth factor reviews, [PMID:10647776] |
| 9. Hata, A A and 5 more authors.. (2000) OAZ uses distinct DNA- and protein-binding zinc fingers in separate BMP-Smad and Olf signaling pathways.. Cell, (21): [PMID:10660046] |
| 10. Wrana, J L JL and Attisano, L L.. () The Smad pathway.. Cytokine & growth factor reviews, [PMID:10708948] |
| 11. Miyazono, K K.. () TGF-beta signaling by Smad proteins.. Cytokine & growth factor reviews, [PMID:10708949] |
| 12. Qin, B Y BY and 5 more authors.. (2001) Structural basis of Smad1 activation by receptor kinase phosphorylation.. Molecular cell, [PMID:11779505] |
| 13. Lin, Yin Y and 11 more authors.. (2002) A novel link between the proteasome pathway and the signal transduction pathway of the bone morphogenetic proteins (BMPs).. BMC cell biology, (21): [PMID:12097147] |
| 14. Bond, Heather M HM and 13 more authors.. (2004) Early hematopoietic zinc finger protein (EHZF), the human homolog to mouse Evi3, is highly expressed in primitive human hematopoietic cells.. Blood, (15): [PMID:14630787] |
| 15. Pan, Deng D and 6 more authors.. (2005) The integral inner nuclear membrane protein MAN1 physically interacts with the R-Smad proteins to repress signaling by the transforming growth factor-{beta} superfamily of cytokines.. The Journal of biological chemistry, (22): [PMID:15647271] |
| 16. Arndt, Stephanie S, Poser, Ina I, Moser, Markus M and Bosserhoff, Anja-Katrin AK.. (2007) Fussel-15, a novel Ski/Sno homolog protein, antagonizes BMP signaling.. Molecular and cellular neurosciences, [PMID:17292623] |
| 17. Kaneko, Satoshi S and 9 more authors.. (2011) Smad inhibition by the Ste20 kinase Misshapen.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:21690388] |
| 18. Nasim, Md Talat MT and 21 more authors.. (2011) Molecular genetic characterization of SMAD signaling molecules in pulmonary arterial hypertension.. Human mutation, [PMID:21898662] |
| 19. Inui, Masafumi M and 11 more authors.. (2011) USP15 is a deubiquitylating enzyme for receptor-activated SMADs.. Nature cell biology, (25): [PMID:21947082] |
| 20. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 21. Vogt, Janis J and 7 more authors.. (2014) Protein associated with SMAD1 (PAWS1/FAM83G) is a substrate for type I bone morphogenetic protein receptors and modulates bone morphogenetic protein signalling.. Open biology, (19): [PMID:24554596] |
| 22. Chen, Fenfang F and 9 more authors.. (2015) Nuclear Export of Smads by RanBP3L Regulates Bone Morphogenetic Protein Signaling and Mesenchymal Stem Cell Differentiation.. Molecular and cellular biology, [PMID:25755279] |
