Basic Description

Product Name	Recombinant Smad4 Antibody - Primary antibody, specific to SMAD4, Rabbit IgG
Synonyms	Deletion target in pancreatic carcinoma 4 \| hSMAD4 \| MAD homolog 4 \| Mothers against DPP homolog 4 \| SMAD 4 \| SMAD family member 4 \| Smad4
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.6 mg/mL
Host species	Rabbit
Specificity	SMAD4
Immunogen	A synthetic peptide derived from human Smad4 (AA 316-552).
Positive Control	WB: NIH/3T3, C6, Ramos ,HUVEC and A549 cell lysates. ICC/IF: HeLa cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Smad4 Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Immunogen affinity purified
Form	Liquid
Concentration	0.6 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Smad4 Antibody (Ab128223) - Western Blot
All lanes: Recombinant Smad4 Antibody (Ab128223) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 60 kDa
Observed band size: 63 kDa
Exposure time: 4.3 s

Recombinant Smad4 Antibody (Ab128223) - IF/ICC
Immunocytochemistry analysis of Smad4 (green) in HeLa cells using Recombinant Smad4 Antibody (Ab128223), and cells were counterstained with DAPI (blue).

Associated Targets(Human)

SMAD4 Tbio Mothers against decapentaplegic homolog 4 (0 Activities)

Discover Target: SMAD4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000
IF/ICC	1/50-1/200 "> 1/50-1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0901183	Certificate of Analysis	Oct 07, 2023	Ab128223
ZJ23F0901184	Certificate of Analysis	Oct 07, 2023	Ab128223

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e]
2. Hahn, S A SA and 10 more authors.. (1996) DPC4, a candidate tumor suppressor gene at human chromosome 18q21.1.. Science (New York, N.Y.), (19): [PMID:8553070]
3. Zhang, Y Y, Feng, X X, We, R R and Derynck, R R.. (1996) Receptor-associated Mad homologues synergize as effectors of the TGF-beta response.. Nature, (12): [PMID:8774881]
4. Moskaluk, C A CA and 11 more authors.. (1997) Genomic sequencing of DPC4 in the analysis of familial pancreatic carcinoma.. Diagnostic molecular pathology : the American journal of surgical pathology, part B, [PMID:9098646]
5. Shi, Y Y, Hata, A A, Lo, R S RS, Massagué, J J and Pavletich, N P NP.. (1997) A structural basis for mutational inactivation of the tumour suppressor Smad4.. Nature, (3): [PMID:9214508]
6. Liu, F F, Pouponnot, C C and Massagué, J J.. (1997) Dual role of the Smad4/DPC4 tumor suppressor in TGFbeta-inducible transcriptional complexes.. Genes & development, (1): [PMID:9389648]
7. Shioda, T T and 8 more authors.. (1998) Transcriptional activating activity of Smad4: roles of SMAD hetero-oligomerization and enhancement by an associating transactivator.. Proceedings of the National Academy of Sciences of the United States of America, (18): [PMID:9707553]
8. Houlston, R R and 18 more authors.. (1998) Mutations in DPC4 (SMAD4) cause juvenile polyposis syndrome, but only account for a minority of cases.. Human molecular genetics, [PMID:9811934]
9. de Caestecker, M P MP and 8 more authors.. (2000) The Smad4 activation domain (SAD) is a proline-rich, p300-dependent transcriptional activation domain.. The Journal of biological chemistry, (21): [PMID:10636916]
10. Qin, B B, Lam, S S SS and Lin, K K.. (1999) Crystal structure of a transcriptionally active Smad4 fragment.. Structure (London, England : 1993), (15): [PMID:10647180]
11. Hata, A A and 5 more authors.. (2000) OAZ uses distinct DNA- and protein-binding zinc fingers in separate BMP-Smad and Olf signaling pathways.. Cell, (21): [PMID:10660046]
12. Chacko, B M BM and 5 more authors.. (2001) The L3 loop and C-terminal phosphorylation jointly define Smad protein trimerization.. Nature structural biology, [PMID:11224571]
13. Smith, D P DP and 5 more authors.. (2001) LIP1, a cytoplasmic protein functionally linked to the Peutz-Jeghers syndrome kinase LKB1.. Human molecular genetics, (1): [PMID:11741830]
14. Wan, Mei M and 7 more authors.. (2002) Jab1 antagonizes TGF-beta signaling by inducing Smad4 degradation.. EMBO reports, [PMID:11818334]
15. Jiao, Kai K, Zhou, Yingna Y and Hogan, Brigid L M BL.. (2002) Identification of mZnf8, a mouse Krüppel-like transcriptional repressor, as a novel nuclear interaction partner of Smad1.. Molecular and cellular biology, [PMID:12370310]
16. Sayed, M G MG and 11 more authors.. (2002) Germline SMAD4 or BMPR1A mutations and phenotype of juvenile polyposis.. Annals of surgical oncology, [PMID:12417513]
17. Felici, Angelina A and 7 more authors.. (2003) TLP, a novel modulator of TGF-beta signaling, has opposite effects on Smad2- and Smad3-dependent signaling.. The EMBO journal, (1): [PMID:12941698]
18. Wu, Kongming K and 11 more authors.. (2003) DACH1 inhibits transforming growth factor-beta signaling through binding Smad4.. The Journal of biological chemistry, (19): [PMID:14525983]
19. Bond, Heather M HM and 13 more authors.. (2004) Early hematopoietic zinc finger protein (EHZF), the human homolog to mouse Evi3, is highly expressed in primitive human hematopoietic cells.. Blood, (15): [PMID:14630787]
20. Chiba, Shigeru S and 9 more authors.. (2003) Homeoprotein DLX-1 interacts with Smad4 and blocks a signaling pathway from activin A in hematopoietic cells.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:14671321]
21. Gallione, Carol J CJ and 9 more authors.. (2004) A combined syndrome of juvenile polyposis and hereditary haemorrhagic telangiectasia associated with mutations in MADH4 (SMAD4).. Lancet (London, England), (13): [PMID:15031030]
22. Chacko, Benoy M BM and 7 more authors.. (2004) Structural basis of heteromeric smad protein assembly in TGF-beta signaling.. Molecular cell, (10): [PMID:15350224]
23. Chen, Hong Bing HB, Rud, Jonathan G JG, Lin, Kai K and Xu, Lan L.. (2005) Nuclear targeting of transforming growth factor-beta-activated Smad complexes.. The Journal of biological chemistry, (3): [PMID:15799969]
24. Choi, So-Jung SJ and 5 more authors.. (2005) Tsc-22 enhances TGF-beta signaling by associating with Smad4 and induces erythroid cell differentiation.. Molecular and cellular biochemistry, [PMID:15881652]
25. Li, Xiaomeng X, Thyssen, Gregory G, Beliakoff, Jason J and Sun, Zijie Z.. (2006) The novel PIAS-like protein hZimp10 enhances Smad transcriptional activity.. The Journal of biological chemistry, (18): [PMID:16777850]
26. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974]
27. Sun, Yan Y and 9 more authors.. (2006) Potentiation of Smad-mediated transcriptional activation by the RNA-binding protein RBPMS.. Nucleic acids research, [PMID:17099224]
28. Seong, Hyun-A HA, Jung, Haiyoung H, Kim, Kyong-Tai KT and Ha, Hyunjung H.. (2007) 3-Phosphoinositide-dependent PDK1 negatively regulates transforming growth factor-beta-induced signaling in a kinase-dependent manner through physical interaction with Smad proteins.. The Journal of biological chemistry, (20): [PMID:17327236]
29. Varelas, Xaralabos X and 8 more authors.. (2008) TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal.. Nature cell biology, [PMID:18568018]
30. Dupont, Sirio S and 15 more authors.. (2009) FAM/USP9x, a deubiquitinating enzyme essential for TGFbeta signaling, controls Smad4 monoubiquitination.. Cell, (9): [PMID:19135894]
31. Nasim, Md Talat MT and 21 more authors.. (2011) Molecular genetic characterization of SMAD signaling molecules in pulmonary arterial hypertension.. Human mutation, [PMID:21898662]
32. Le Goff, Carine C and 16 more authors.. (2011) Mutations at a single codon in Mad homology 2 domain of SMAD4 cause Myhre syndrome.. Nature genetics, (11): [PMID:22158539]
33. Caputo, Viviana V and 18 more authors.. (2012) A restricted spectrum of mutations in the SMAD4 tumor-suppressor gene underlies Myhre syndrome.. American journal of human genetics, (13): [PMID:22243968]
34. Feng, Yili Y and 6 more authors.. (2014) Zinc finger protein 451 is a novel Smad corepressor in transforming growth factor-β signaling.. The Journal of biological chemistry, (24): [PMID:24324267]
35. Chen, Qiuyue Q, Lee, Ching-En CE, Denard, Bray B and Ye, Jin J.. (2014) Sustained induction of collagen synthesis by TGF-β requires regulated intramembrane proteolysis of CREB3L1.. PloS one, [PMID:25310401]
36. Yang, Yutao Y and 10 more authors.. (2015) Pokemon (FBI-1) interacts with Smad4 to repress TGF-β-induced transcriptional responses.. Biochimica et biophysica acta, [PMID:25514493]
37. Braun, Daniela A DA and 26 more authors.. (2016) Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause steroid-resistant nephrotic syndrome.. Nature genetics, [PMID:26878725]

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SKU	Size	Availability	Price
Ab128223-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$86.90
Ab128223-50μl	50μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$249.90
Ab128223-100μl	100μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$349.90
Ab128223-1ml	1ml	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$2,699.90

Recombinant Smad4 Antibody - Primary antibody, specific to SMAD4, Rabbit IgG