Recombinant; Rabbit anti Human Smad4 Antibody; WB, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab128223-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $86.90 | |
| Ab128223-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $249.90 | |
| Ab128223-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $349.90 | |
| Ab128223-1ml | 1ml | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $2,699.90 |
Recombinant; Rabbit anti Human Smad4 Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant Smad4 Antibody - Primary antibody, specific to SMAD4, Rabbit IgG |
|---|---|
| Synonyms | SMAD4; DPC4; MADH4; Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4; (Small) Mothers Against Decapentaplegic antibody; Deleted |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | SMAD4 |
| Immunogen | A synthetic peptide derived from human Smad4 (AA 316-552). |
| Positive Control | WB: NIH/3T3, C6, Ramos ,HUVEC and A549 cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Smad4 Antibody, Recombinant, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Smad4 Antibody (Ab128223) - Western Blot
All lanes: Recombinant Smad4 Antibody (Ab128223) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 63 kDa
Exposure time: 4.3 s
Recombinant Smad4 Antibody (Ab128223) - IF/ICC
Immunocytochemistry analysis of Smad4 (green) in HeLa cells using Recombinant Smad4 Antibody (Ab128223), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901183 | Certificate of Analysis | Oct 07, 2023 | Ab128223 |
| ZJ23F0901184 | Certificate of Analysis | Oct 07, 2023 | Ab128223 |
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| 37. Braun, Daniela A DA and 26 more authors.. (2016) Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause steroid-resistant nephrotic syndrome.. Nature genetics, [PMID:26878725] |
