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Ab128272-10μl

10μl

In stock

$69.90

Ab128272-50μl

50μl

In stock

$189.90

Ab128272-100μl

100μl

Available within 8-12 weeks(?)

$299.90

Ab128272-1ml

1ml

Available within 8-12 weeks(?)

$2,699.90

Basic Description

Product Name	Recombinant SMARCC1/BAF155 Antibody - Primary antibody, specific to SMARCC1, Rabbit IgG
Synonyms	BAF155 \| BRG1-associated factor 155 \| SWI/SNF complex 155 kDa subunit \| SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 \| AI115498antibody \| BAF 155 antibody \| BAF155 antibody \| BRG 1 associated factor 155 ant
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.4 mg/mL
Host species	Rabbit
Specificity	SMARCC1
Immunogen	A synthetic peptide derived from human SMARCC1 (AA 729-742)
Positive Control	WB: HeLa, Hep G2, NIH/3T3 and C6 cell lysates. ICC/IF: HT-1080 cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.4 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SMARCC1/BAF155 Antibody (Ab128272) - Western Blot
All lanes: Recombinant SMARCC1/BAF155 Antibody (Ab128272) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 123 kDa
Observed band size: 154 kDa
Exposure time: 38.9 s

Recombinant SMARCC1/BAF155 Antibody (Ab128272) - IF/ICC
Immunocytochemistry analysis of SMARCC1 (green) in HT-1080 using Recombinant SMARCC1/BAF155 Antibody (Ab128272), and cells were counterstained with DAPI (blue).

Associated Targets(Human)

SMARCC1 Tbio SWI/SNF complex subunit SMARCC1 (0 Activities)

Discover Target: SMARCC1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000
IF/ICC	1/50-1/200 "> 1/50-1/200

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0403950	Certificate of Analysis	Apr 01, 2024	Ab128272
ZJ24F0403951	Certificate of Analysis	Apr 01, 2024	Ab128272

FAQs and Articles

FAQ

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: Protocols

Tags:

Frozen section Immunofluorescence staining Tissue section Methanol permeability IF

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e]
2. Hsiao PW, Fryer CJ, Trotter KW, Wang W, Archer TK. (2003) BAF60a mediates critical interactions between nuclear receptors and the BRG1 chromatin-remodeling complex for transactivation.. Mol Cell Biol, 23 (17): (6210-20). [PMID:12917342] [10.1021/op500134e]
3. Kadoch C, Crabtree GR. (2015) Mammalian SWI/SNF chromatin remodeling complexes and cancer: Mechanistic insights gained from human genomics.. Sci Adv, 1 (5): (e1500447). [PMID:26601204] [10.1021/op500134e]
4. Wang, W W and 5 more authors.. (1996) Diversity and specialization of mammalian SWI/SNF complexes.. Genes & development, (1): [PMID:8804307]
5. Sif, S S, Stukenberg, P T PT, Kirschner, M W MW and Kingston, R E RE.. (1998) Mitotic inactivation of a human SWI/SNF chromatin remodeling complex.. Genes & development, (15): [PMID:9744861]
6. Shanahan, F F, Seghezzi, W W, Parry, D D, Mahony, D D and Lees, E E.. (1999) Cyclin E associates with BAF155 and BRG1, components of the mammalian SWI-SNF complex, and alters the ability of BRG1 to induce growth arrest.. Molecular and cellular biology, [PMID:9891079]
7. Phelan, M L ML, Sif, S S, Narlikar, G J GJ and Kingston, R E RE.. (1999) Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.. Molecular cell, [PMID:10078207]
8. Kadam, S S and 5 more authors.. (2000) Functional selectivity of recombinant mammalian SWI/SNF subunits.. Genes & development, (1): [PMID:11018012]
9. Sif, S S, Saurin, A J AJ, Imbalzano, A N AN and Kingston, R E RE.. (2001) Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes.. Genes & development, (1): [PMID:11238380]
10. Brill, Laurence M LM and 5 more authors.. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.. Analytical chemistry, (15): [PMID:15144186]
11. Lange, Martin M and 16 more authors.. (2008) Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex.. Genes & development, (1): [PMID:18765789]
12. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
13. Kowenz-Leutz, Elisabeth E, Pless, Ole O, Dittmar, Gunnar G, Knoblich, Maria M and Leutz, Achim A.. (2010) Crosstalk between C/EBPbeta phosphorylation, arginine methylation, and SWI/SNF/Mediator implies an indexing transcription factor code.. The EMBO journal, (17): [PMID:20111005]
14. Keppler, Brian R BR and Archer, Trevor K TK.. (2010) Ubiquitin-dependent and ubiquitin-independent control of subunit stoichiometry in the SWI/SNF complex.. The Journal of biological chemistry, (12): [PMID:20829358]
15. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
16. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
17. Euskirchen, Ghia G, Auerbach, Raymond K RK and Snyder, Michael M.. (2012) SWI/SNF chromatin-remodeling factors: multiscale analyses and diverse functions.. The Journal of biological chemistry, (7): [PMID:22952240]
18. Huber, Ferdinand M FM and 7 more authors.. (2017) Histone-binding of DPF2 mediates its repressive role in myeloid differentiation.. Proceedings of the National Academy of Sciences of the United States of America, (6): [PMID:28533407]
19. Scott, Charles Anthony CA and 8 more authors.. (2017) Nuclear/cytoplasmic transport defects in BBS6 underlie congenital heart disease through perturbation of a chromatin remodeling protein.. PLoS genetics, [PMID:28753627]
20. Alpsoy, Aktan A and Dykhuizen, Emily C EC.. (2018) Glioma tumor suppressor candidate region gene 1 (GLTSCR1) and its paralog GLTSCR1-like form SWI/SNF chromatin remodeling subcomplexes.. The Journal of biological chemistry, (16): [PMID:29374058]
21. Furey, Charuta Gavankar CG and 44 more authors.. (2018) De Novo Mutation in Genes Regulating Neural Stem Cell Fate in Human Congenital Hydrocephalus.. Neuron, (25): [PMID:29983323]

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Recombinant SMARCC1/BAF155 Antibody - Primary antibody, specific to SMARCC1, Rabbit IgG