Basic Description

Product Name	Recombinant SNF5/SMARCB1 Antibody - Primary antibody, specific to SMARCB1, Rabbit IgG
Synonyms	BAF47 antibody \| BRG1-associated factor 47 antibody \| hSNF5 antibody \| INI1 antibody \| Integrase interactor 1 protein antibody \| Malignant rhabdoid tumor suppressor antibody \| RDT antibody \| RTPS1 antibody \| Sfh1p antibody \| SMARCB1 antibody \| SNF5 homolo
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.6 mg/mL
Host species	Rabbit
Specificity	SMARCB1
Immunogen	Recombinant full length protein (Human)
Positive Control	WB: MCF7, HeLa, K562, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human SMARCB1 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.6 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SNF5/SMARCB1 Antibody (Ab128494) - Western Blot
All lanes: Recombinant SNF5/SMARCB1 Antibody (Ab128494) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 42 kDa
Exposure time: 12.0 s

Recombinant SNF5/SMARCB1 Antibody (Ab128494) - IF/ICC
Immunocytochemistry analysis of SNF5 (green) in HeLa using Recombinant SNF5/SMARCB1 Antibody (Ab128494), and cells were counterstained with DAPI (blue).

Recombinant SNF5/SMARCB1 Antibody (Ab128494) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant SNF5/SMARCB1 Antibody (Ab128494). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

SMARCB1 Tbio SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 (0 Activities)

Discover Target: SMARCB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000
IF/ICC	1/50-1/200 "> 1/50-1/200
IHC	1/50 - 1/100 "> 1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0403954	Certificate of Analysis	Apr 01, 2024	Ab128494
ZJ24F0403955	Certificate of Analysis	Apr 01, 2024	Ab128494

FAQs and Articles

References

1. Kadoch C, Crabtree GR. (2015) Mammalian SWI/SNF chromatin remodeling complexes and cancer: Mechanistic insights gained from human genomics.. Sci Adv, 1 (5): (e1500447). [PMID:26601204] [10.1021/op500134e]
2. Kalpana, G V GV, Marmon, S S, Wang, W W, Crabtree, G R GR and Goff, S P SP.. (1994) Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5.. Science (New York, N.Y.), (23): [PMID:7801128]
3. Wu, D Y DY, Kalpana, G V GV, Goff, S P SP and Schubach, W H WH.. (1996) Epstein-Barr virus nuclear protein 2 (EBNA2) binds to a component of the human SNF-SWI complex, hSNF5/Ini1.. Journal of virology, [PMID:8709224]
4. Wang, W W and 11 more authors.. (1996) Purification and biochemical heterogeneity of the mammalian SWI-SNF complex.. The EMBO journal, (1): [PMID:8895581]
5. Morozov, A A, Yung, E E and Kalpana, G V GV.. (1998) Structure-function analysis of integrase interactor 1/hSNF5L1 reveals differential properties of two repeat motifs present in the highly conserved region.. Proceedings of the National Academy of Sciences of the United States of America, (3): [PMID:9448295]
6. Versteege, I I and 7 more authors.. (1998) Truncating mutations of hSNF5/INI1 in aggressive paediatric cancer.. Nature, (9): [PMID:9671307]
7. Biegel, J A JA and 5 more authors.. (1999) Germ-line and acquired mutations of INI1 in atypical teratoid and rhabdoid tumors.. Cancer research, (1): [PMID:9892189]
8. Phelan, M L ML, Sif, S S, Narlikar, G J GJ and Kingston, R E RE.. (1999) Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.. Molecular cell, [PMID:10078207]
9. Bruder, C E CE, Dumanski, J P JP and Kedra, D D.. (1999) The mouse ortholog of the human SMARCB1 gene encodes two splice forms.. Biochemical and biophysical research communications, (21): [PMID:10208879]
10. Cheng, S W SW and 5 more authors.. (1999) c-MYC interacts with INI1/hSNF5 and requires the SWI/SNF complex for transactivation function.. Nature genetics, [PMID:10319872]
11. Lee, D D, Sohn, H H, Kalpana, G V GV and Choe, J J.. (1999) Interaction of E1 and hSNF5 proteins stimulates replication of human papillomavirus DNA.. Nature, (3): [PMID:10365963]
12. Adler, H T HT and 6 more authors.. (1999) Leukemic HRX fusion proteins inhibit GADD34-induced apoptosis and associate with the GADD34 and hSNF5/INI1 proteins.. Molecular and cellular biology, [PMID:10490642]
13. Wu, Daniel Y DY, Tkachuck, Douglas C DC, Roberson, Rachel S RS and Schubach, William H WH.. (2002) The human SNF5/INI1 protein facilitates the function of the growth arrest and DNA damage-inducible protein (GADD34) and modulates GADD34-bound protein phosphatase-1 activity.. The Journal of biological chemistry, (2): [PMID:12016208]
14. Versteege, Isabella I, Medjkane, Souhila S, Rouillard, Danny D and Delattre, Olivier O.. (2002) A key role of the hSNF5/INI1 tumour suppressor in the control of the G1-S transition of the cell cycle.. Oncogene, (19): [PMID:12226744]
15. Oruetxebarria, Igor I and 8 more authors.. (2004) P16INK4a is required for hSNF5 chromatin remodeler-induced cellular senescence in malignant rhabdoid tumor cells.. The Journal of biological chemistry, (30): [PMID:14604992]
16. Collins, John E JE and 12 more authors.. (2004) A genome annotation-driven approach to cloning the human ORFeome.. Genome biology, [PMID:15461802]
17. Modena, Piergiorgio P and 6 more authors.. (2005) SMARCB1/INI1 tumor suppressor gene is frequently inactivated in epithelioid sarcomas.. Cancer research, (15): [PMID:15899790]
18. Pan, Xuefang X, Song, Zhaoxia Z, Zhai, Lei L, Li, Xiaoyun X and Zeng, Xianlu X.. (2005) Chromatin-remodeling factor INI1/hSNF5/BAF47 is involved in activation of the colony stimulating factor 1 promoter.. Molecules and cells, (31): [PMID:16267391]
19. Ulyanova, Natalia P NP and Schnitzler, Gavin R GR.. (2005) Human SWI/SNF generates abundant, structurally altered dinucleosomes on polynucleosomal templates.. Molecular and cellular biology, [PMID:16314535]
20. Angrand, Pierre-Olivier PO and 12 more authors.. (2006) Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.. Molecular & cellular proteomics : MCP, [PMID:16959763]
21. Hulsebos, Theo J M TJ and 5 more authors.. (2007) Germline mutation of INI1/SMARCB1 in familial schwannomatosis.. American journal of human genetics, [PMID:17357086]
22. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332]
23. Sestini, Roberta R, Bacci, Costanza C, Provenzano, Aldesia A, Genuardi, Maurizio M and Papi, Laura L.. (2008) Evidence of a four-hit mechanism involving SMARCB1 and NF2 in schwannomatosis-associated schwannomas.. Human mutation, [PMID:18072270]
24. Lange, Martin M and 16 more authors.. (2008) Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex.. Genes & development, (1): [PMID:18765789]
25. Kowenz-Leutz, Elisabeth E, Pless, Ole O, Dittmar, Gunnar G, Knoblich, Maria M and Leutz, Achim A.. (2010) Crosstalk between C/EBPbeta phosphorylation, arginine methylation, and SWI/SNF/Mediator implies an indexing transcription factor code.. The EMBO journal, (17): [PMID:20111005]
26. Tando, Toshio T and 11 more authors.. (2010) Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway.. The Journal of biological chemistry, (16): [PMID:20460684]
27. Zhai, Niu N and 8 more authors.. (2012) Human PIH1 associates with histone H4 to mediate the glucose-dependent enhancement of pre-rRNA synthesis.. Journal of molecular cell biology, [PMID:22368283]
28. Tsurusaki, Yoshinori Y and 30 more authors.. (2012) Mutations affecting components of the SWI/SNF complex cause Coffin-Siris syndrome.. Nature genetics, (18): [PMID:22426308]
29. Kleefstra, Tjitske T and 21 more authors.. (2012) Disruption of an EHMT1-associated chromatin-modification module causes intellectual disability.. American journal of human genetics, (13): [PMID:22726846]
30. Euskirchen, Ghia G, Auerbach, Raymond K RK and Snyder, Michael M.. (2012) SWI/SNF chromatin-remodeling factors: multiscale analyses and diverse functions.. The Journal of biological chemistry, (7): [PMID:22952240]
31. Wieczorek, Dagmar D and 55 more authors.. (2013) A comprehensive molecular study on Coffin-Siris and Nicolaides-Baraitser syndromes identifies a broad molecular and clinical spectrum converging on altered chromatin remodeling.. Human molecular genetics, (20): [PMID:23906836]
32. Ciaffardini, F F and 8 more authors.. (2014) The cockayne syndrome B protein is essential for neuronal differentiation and neuritogenesis.. Cell death & disease, (29): [PMID:24874740]
33. Allen, Mark D MD, Freund, Stefan M V SM, Zinzalla, Giovanna G and Bycroft, Mark M.. (2015) The SWI/SNF Subunit INI1 Contains an N-Terminal Winged Helix DNA Binding Domain that Is a Target for Mutations in Schwannomatosis.. Structure (London, England : 1993), (7): [PMID:26073604]

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SKU	Size	Availability	Price
Ab128494-10μl	10μl	In stock	$69.90
Ab128494-50μl	50μl	In stock	$189.90
Ab128494-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab128494-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,699.90

Recombinant SNF5/SMARCB1 Antibody - Primary antibody, specific to SMARCB1, Rabbit IgG