Basic Description

Product Name	Recombinant SOX10 Antibody - Primary antibody, specific to SOX10, Rabbit IgG
Synonyms	DOMantibody \| DOM antibody \| Dominant megacolon mouse human homolog of antibody \| MGC15649 antibody \| PCWH antibody \| SOX 10 antibody \| SOX10 antibody \| SOX10_HUMAN antibody \| SRY (sex determining region Y) box 10antibody \| SRY (sex determining region Y)
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.273 mg/mL
Host species	Rabbit
Specificity	SOX10
Immunogen	A synthetic peptide corresponding to SOX10 residues within aa400-500 of SOX10 was used as an immunogen.
Positive Control	WB: A375 and C6 cell lysates. IHC: Human melanoma tissue. Flow: A375 cells. ICC/IF: A375 cells. IP: A375 whole cell lysate
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human SOX10 Antibody, Primary antibody, could be used for WB, IHC, Flow, IF/ICC, IP and so on. Application: WB: 1/1000 - 1/2000 IHC: 1/1000 - 1/2000 Flow: 1/200 - 1/1000 IF/ICC: 1/2000 - 1/10000 IP: 1/50 Function Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	0.273 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C for 2 years. Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant SOX10 Antibody (Ab128734) - Western Blot
All lanes: Recombinant SOX10 Antibody (Ab128734) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 49 kDa
Observed band size: 57, 70 kDa
Exposure time: 1.1 s

Recombinant SOX10 Antibody (Ab128734) - IF/ICC
Recombinant SOX10 Antibody (Ab128734) staining SOX10 in A375 cells by IF/ICC. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant SOX10 Antibody (Ab128734) (1/10000) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1/500).

Recombinant SOX10 Antibody ( Ab128734 ) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling SOX10 with Recombinant SOX10 Antibody (Ab128734) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Recombinant SOX10 Antibody (Ab128734) - IP
SOX10 was immunoprecipitated from 0.4 mg of A375 cell lysate with Recombinant SOX10 Antibody (Ab128734) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant SOX10 Antibody (Ab128734) IP in A375 whole cell lysate
Lane 2: Rabbit IgG instead of Recombinant SOX10 Antibody (Ab128734) in A375 whole cell lysate
Lane3: A375 whole cell lysate, 10 μg (input)
Exposure: 120.0 s.

Recombinant SOX10 Antibody (Ab128734) - Flow Cytometry
Overlay histogram showing A375 cells stained with Recombinant SOX10 Antibody (Ab128734)(blue). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant SOX10 Antibody (Ab128734) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (red) was used as a control.