Recombinant (RR643); Rabbit anti Human SOX10 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab128734-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 |
Recombinant (RR643); Rabbit anti Human SOX10 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
| Product Name | Recombinant SOX10 Antibody - Primary antibody, specific to SOX10, Rabbit IgG |
|---|---|
| Synonyms | DOMantibody; DOM antibody; Dominant megacolon mouse human homolog of antibody; MGC15649 antibody; PCWH antibody; SOX 10 antibody; SOX10 antibody; SOX10_HUMAN antibody; SRY (sex determining region Y) box 10antibody; SRY (sex determining region Y) box 10 an |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.273 mg/mL |
| Host species | Rabbit |
| Specificity | SOX10 |
| Immunogen | A synthetic peptide corresponding to SOX10 residues within aa400-500 of SOX10 was used as an immunogen. |
| Positive Control | WB: A375 and C6 cell lysates. IHC: Human melanoma tissue. Flow: A375 cells. ICC/IF: A375 cells. IP: A375 whole cell lysate |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human SOX10 Antibody, Primary antibody, could be used for WB, IHC, Flow, IF/ICC, IP and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.273 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C for 2 years. Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant SOX10 Antibody (Ab128734) - Western Blot
All lanes: Recombinant SOX10 Antibody (Ab128734) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 57, 70 kDa
Exposure time: 1.1 s
Recombinant SOX10 Antibody (Ab128734) - IF/ICC
Recombinant SOX10 Antibody (Ab128734) staining SOX10 in A375 cells by IF/ICC. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant SOX10 Antibody (Ab128734) (1/10000) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant SOX10 Antibody (
Ab128734
) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling SOX10 with Recombinant SOX10 Antibody (Ab128734) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant SOX10 Antibody (Ab128734) - IP
SOX10 was immunoprecipitated from 0.4 mg of A375 cell lysate with Recombinant SOX10 Antibody (Ab128734) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant SOX10 Antibody (Ab128734) IP in A375 whole cell lysate
Lane 2: Rabbit IgG instead of Recombinant SOX10 Antibody (Ab128734) in A375 whole cell lysate
Lane3: A375 whole cell lysate, 10 μg (input)
Exposure: 120.0 s.
Recombinant SOX10 Antibody (Ab128734) - Flow Cytometry
Overlay histogram showing A375 cells stained with Recombinant SOX10 Antibody (Ab128734)(blue). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant SOX10 Antibody (Ab128734) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (red) was used as a control.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB |
1/1000 - 1/2000
|
| IP |
1/50
|
| Flow |
1/200 - 1/1000
|
| IF/ICC |
1/200-1/1000
|
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1201988 | Certificate of Analysis | Dec 13, 2023 | Ab128734 |
| ZJ23F1201987 | Certificate of Analysis | Dec 13, 2023 | Ab128734 |
