| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab129486-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab129486-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $179.90 | |
| Ab129486-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $299.90 | |
| Ab129486-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
Recombinant; Rabbit anti Human STAT6 Antibody; WB; Unconjugated
| Product Name | Recombinant STAT6 Antibody - Primary antibody, specific to STAT6, Rabbit IgG |
|---|---|
| Synonyms | 12S1644 antibody | D12S1644 antibody | IL 4 STAT antibody | IL-4 Stat antibody | IL4 STAT antibody | Interleukin 4 Induced antibody | Interleukin 4 Induced Transcription Factor IL4 STAT antibody | Signal transducer and activator of transcription 6 antibod |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | STAT6 |
| Immunogen | A synthetic peptide derived from human STAT6 (AA 637-647) |
| Positive Control | WB: NIH/3T3, Ramos and HeLa cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human STAT6 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant STAT6 Antibody (Ab129486) - Western Blot
All lanes: Recombinant STAT6 Antibody (Ab129486) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 96 kDa
Exposure time: 20.4 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 "> 1/500-1/2000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001282 | Certificate of Analysis | Oct 13, 2023 | Ab129486 |
| ZJ23F1001283 | Certificate of Analysis | Oct 13, 2023 | Ab129486 |
| 1. Hou J, Schindler U, Henzel WJ, Ho TC, Brasseur M, McKnight SL. (1994) An interleukin-4-induced transcription factor: IL-4 Stat.. Science, 265 (5179): (1701-6). [PMID:8085155] [10.1021/op500134e] |
| 2. Mandal PK, Morlacchi P, Knight JM, Link TM, Lee 4th GR, Nurieva R, Singh D, Dhanik A, Kavraki L, Corry DB et al.. (2015) Targeting the Src Homology 2 (SH2) Domain of Signal Transducer and Activator of Transcription 6 (STAT6) with Cell-Permeable, Phosphatase-Stable Phosphopeptide Mimics Potently Inhibits Tyr641 Phosphorylation and Transcriptional Activity.. J Med Chem, 58 (22): (8970-84). [PMID:26506089] [10.1021/op500134e] |
| 3. Shinya Nagashima,Hiroshi Nagata,Masahiro Iwata,Masaki Yokota,Hiroyuki Moritomo,Masaya Orita,Sadao Kuromitsu,Akiko Koakutsu,Keiko Ohga,Makoto Takeuchi,Mitsuaki Ohta,Shin-ichi Tsukamoto. (2008-06-07) Identification of 4-benzylamino-2-[(4-morpholin-4-ylphenyl)amino]pyrimidine-5-carboxamide derivatives as potent and orally bioavailable STAT6 inhibitors.. Bioorganic & medicinal chemistry, 16 ((13)): (6509-6521). [PMID:18534856] |
| 4. Nagashima S, Yokota M, Nakai E, Kuromitsu S, Ohga K, Takeuchi M, Tsukamoto S, Ohta M.. (2007) Synthesis and evaluation of 2-{[2-(4-hydroxyphenyl)-ethyl]amino}pyrimidine-5-carboxamide derivatives as novel STAT6 inhibitors.. Bioorg Med Chem, 15 (2): (1044-1055). [PMID:17071093] [10.1016/j.bmc.2006.10.015] |
| 5. Nagashima S, Hondo T, Nagata H, Ogiyama T, Maeda J, Hoshii H, Kontani T, Kuromitsu S, Ohga K, Orita M, Ohno K, Moritomo A, Shiozuka K, Furutani M, Takeuchi M, Ohta M, Tsukamoto S.. (2009) Novel 7H-pyrrolo[2,3-d]pyrimidine derivatives as potent and orally active STAT6 inhibitors.. Bioorg Med Chem, 17 (19): (6926-6936). [PMID:19747833] [10.1016/j.bmc.2009.08.021] |
| 6. Kumar A, Shah BA, Singh S, Hamid A, Singh SK, Sethi VK, Saxena AK, Singh J, Taneja SC.. (2012) Acyl derivatives of boswellic acids as inhibitors of NF-κB and STATs.. Bioorg Med Chem Lett, 22 (1): (431-435). [PMID:22123322] [10.1016/j.bmcl.2011.10.112] |
| 7. Zhou L, Kawate T, Liu X, Kim YB, Zhao Y, Feng G, Banerji J, Nash H, Whitehurst C, Jindal S, Siddiqui A, Seed B, Wolfe JL.. (2012) STAT6 phosphorylation inhibitors block eotaxin-3 secretion in bronchial epithelial cells.. Bioorg Med Chem, 20 (2): (750-758). [PMID:22217933] [10.1016/j.bmc.2011.12.006] |
| 8. Chung HS, Kim Y, Oh SJ, Kim H, Choi SI, Zhang Y, Jeong JH, Bae H.. (2013) A synthetic compound, 4-acetyl-3-methyl-6-(3,4,5-trimethoxyphenyl)pyrano[3,4-c]pyran-1,8-dione, ameliorates ovalbumin-induced asthma.. Bioorg Med Chem, 21 (21): (6359-6365). [PMID:24054491] [10.1016/j.bmc.2013.08.045] |
| 9. Morlacchi P, Mandal PK, McMurray JS.. (2014) Synthesis and in Vitro Evaluation of a Peptidomimetic Inhibitor Targeting the Src Homology 2 (SH2) Domain of STAT6.. ACS Med Chem Lett, 5 (1): (69-72). [PMID:24900775] [10.1021/ml4003919] |
| 10. Zhou H, Bai L, Xu R, Zhao Y, Chen J, McEachern D, Chinnaswamy K, Wen B, Dai L, Kumar P, Yang CY, Liu Z, Wang M, Liu L, Meagher JL, Yi H, Sun D, Stuckey JA, Wang S.. (2019) Structure-Based Discovery of SD-36 as a Potent, Selective, and Efficacious PROTAC Degrader of STAT3 Protein.. J Med Chem, 62 (24): (11280-11300). [PMID:31747516] [10.1021/acs.jmedchem.9b01530] |
| 11. Huang MR, Hsu YL, Lin TC, Cheng TJ, Li LW, Tseng YW, Chou YS, Liu JH, Pan SH, Fang JM, Wong CH.. (2019) Structure-guided development of purine amide, hydroxamate, and amidoxime for the inhibition of non-small cell lung cancer.. Eur J Med Chem, 181 (111551-111551). [PMID:31376567] [10.1016/j.ejmech.2019.07.054] |
| 12. Kaoud TS,Mohassab AM,Hassan HA,Yan C,Van Ravenstein SX,Abdelhamid D,Dalby KN,Abdel-Aziz M. (2020) NO-releasing STAT3 inhibitors suppress BRAF-mutant melanoma growth.. Eur J Med Chem, 186 (111885-111885). [PMID:31784187] [10.1016/j.ejmech.2019.111885] |
| 13. Patel, B K BK, Keck, C L CL, O'Leary, R S RS, Popescu, N C NC and LaRochelle, W J WJ.. (1998) Localization of the human stat6 gene to chromosome 12q13.3-q14.1, a region implicated in multiple solid tumors.. Genomics, (1): [PMID:9782085] |
| 14. Litterst, Claudia M CM and Pfitzner, Edith E.. (2002) An LXXLL motif in the transactivation domain of STAT6 mediates recruitment of NCoA-1/SRC-1.. The Journal of biological chemistry, (27): [PMID:12138096] |
| 15. Razeto, Adelia A and 10 more authors.. (2004) Structure of the NCoA-1/SRC-1 PAS-B domain bound to the LXXLL motif of the STAT6 transactivation domain.. Journal of molecular biology, (13): [PMID:14757047] |
| 16. Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16): [PMID:16541075] |
| 17. Lu, Xiaoqing X and 6 more authors.. (2007) T-cell protein tyrosine phosphatase, distinctively expressed in activated-B-cell-like diffuse large B-cell lymphomas, is the nuclear phosphatase of STAT6.. Molecular and cellular biology, [PMID:17210636] |
| 18. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 19. Iwata, Hiroshi H and 18 more authors.. (2016) PARP9 and PARP14 cross-regulate macrophage activation via STAT1 ADP-ribosylation.. Nature communications, (31): [PMID:27796300] |
