Recombinant; Rabbit anti Human STIP1/STI1 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab129574-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $79.90 | |
| Ab129574-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $229.90 | |
| Ab129574-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab129574-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human STIP1/STI1 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant STIP1/STI1 Antibody - Primary antibody, specific to STIP1, Rabbit IgG |
|---|---|
| Synonyms | Hop | Hsc70/Hsp90-organizing protein | Renal carcinoma antigen NY-REN-11 | STI1 | Transformation-sensitive protein IEF SSP 3521 | Epididymis secretory sperm binding protein Li 94n antibody | HEL S 94n antibody | Hop antibody | Hsc70/Hsp90 organizing prote |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | STIP1 |
| Immunogen | A synthetic peptide derived from human STIP1 (AA 67-80) |
| Positive Control | WB: K562, A431, HEK293, HeLa, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human STIP1/STI1 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant STIP1/STI1 Antibody (Ab129574) - Western Blot
All lanes: Recombinant STIP1/STI1 Antibody (Ab129574) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 64 kDa
Exposure time: 18.7 s
Recombinant STIP1/STI1 Antibody (Ab129574) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant STIP1/STI1 Antibody (Ab129574). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0506169 | Certificate of Analysis | May 28, 2024 | Ab129574 |
| ZJ24F0506168 | Certificate of Analysis | May 28, 2024 | Ab129574 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 3. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211] |
| 4. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 5. Rasmussen, H H HH and 5 more authors.. (1992) Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes.. Electrophoresis, [PMID:1286667] |
| 6. Honoré, B B and 5 more authors.. (1992) Molecular cloning and expression of a transformation-sensitive human protein containing the TPR motif and sharing identity to the stress-inducible yeast protein STI1.. The Journal of biological chemistry, (25): [PMID:1569099] |
| 7. Silverstein, A M AM and 5 more authors.. (1997) Protein phosphatase 5 is a major component of glucocorticoid receptor.hsp90 complexes with properties of an FK506-binding immunophilin.. The Journal of biological chemistry, (27): [PMID:9195923] |
| 8. Scanlan, M J MJ and 11 more authors.. (1999) Antigens recognized by autologous antibody in patients with renal-cell carcinoma.. International journal of cancer, (12): [PMID:10508479] |
| 9. Scheufler, C C and 7 more authors.. (2000) Structure of TPR domain-peptide complexes: critical elements in the assembly of the Hsp70-Hsp90 multichaperone machine.. Cell, (14): [PMID:10786835] |
| 10. Imai, Yuzuru Y and 5 more authors.. (2003) A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death.. The Journal of biological chemistry, (19): [PMID:14532270] |
| 11. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455] |
| 12. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 13. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 14. Cloutier, Philippe P, Lavallée-Adam, Mathieu M, Faubert, Denis D, Blanchette, Mathieu M and Coulombe, Benoit B.. (2013) A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.. PLoS genetics, [PMID:23349634] |
| 15. Hamamoto, Ryuji R, Toyokawa, Gouji G, Nakakido, Makoto M, Ueda, Koji K and Nakamura, Yusuke Y.. (2014) SMYD2-dependent HSP90 methylation promotes cancer cell proliferation by regulating the chaperone complex formation.. Cancer letters, (28): [PMID:24880080] |
| 16. Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V and Vertegaal, Alfred C O.. (2015) SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage.. Cell reports, (17): [PMID:25772364] |
| 17. Woodford, Mark R MR and 16 more authors.. (2016) The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding.. Nature communications, (29): [PMID:27353360] |
| 18. Woodford, Mark R MR and 15 more authors.. (2017) Tumor suppressor Tsc1 is a new Hsp90 co-chaperone that facilitates folding of kinase and non-kinase clients.. The EMBO journal, (15): [PMID:29127155] |
