Recombinant Synaptophysin Antibody - Primary antibody, specific to Synaptophysin, Mouse IgG1
Synonyms
Major synaptic vesicle protein p38 antibody | MRX96 antibody | MRXSYP antibody | Syn p38 antibody | Synaptophysin antibody | Syp antibody | SYPH antibody | SYPH_HUMAN antibody | SypI antibody | Major synaptic vesicle protein p38 | MRX | MRXSYP | Synaptoph
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.48 mg/mL
Host species
Mouse
Specificity
Synaptophysin
Positive Control
IHC: Human Pancreas.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Mouse anti Human Synaptophysin Antibody, Recombinant (MyM1-Syn), could be used for IHC and so on. Application IHC: 1/100-1/200 Protein Function Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
Product Properties
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
28.2 kDa (Light Chain) & 51.1 kDa (Heavy Chain), under reducing conditions; 173.2 kDa, under non-reducing conditions.
Purification Method
Protein A purified
Form
Liquid
Concentration
0.48 mg/mL
Storage Temp
Store at 2-8°C,Avoid repeated freezing and thawing
Shipped In
Wet ice
Stability And Storage
Store at 2-8°C long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Synaptophysin Antibody (Ab129952) - IHC Immunohistochemistry was performed on human pancreatic ductal carcinoma and human appendix tissue. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Following antigen retrieval, tissues were blocked in a 10% goat serum in wash buffer solution for 30 minutes at room temperature and endogenous peroxidase activity quenched with Peroxidase Suppressor. Tissue was then probed with a Recombinant Synaptophysin Antibody (Ab129952) at a dilution of 1:100 in 10% goat serum for overnight at 4℃. Using a Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) as secondary antibody, and detection was performed using a Streptavidin (HRP) (np156148) followed by colorimetric detection using DAB .Tissues were then counterstained with hematoxylin and prepped for mounting and imaging.
Recombinant Synaptophysin Antibody (Ab129952) - SDS-PAGE 3 μg/lane of Recombinant Synaptophysin Antibody (Ab129952) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 27.0 kDa (Light Chain) & 53.2 kDa (Heavy Chain) under reducing conditions and 173.2 kDa under non-reducing conditions.
ZJ23F0901014