A synthetic peptide derived from human TdT (AA 495-509).
Positive Control
WB: MOLT-4, Jurkat and RAW 264.7 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human TdT Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function: Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.2 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant TdT Antibody (Ab130625) - Western Blot All lanes: Recombinant TdT Antibody (Ab130625) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 59 kDa Observed band size: 56 kDa Exposure time: 15.2 s
1.Riley, L K LK, Morrow, J K JK, Danton, M J MJ and Coleman, M S MS.. (1988) Human terminal deoxyribonucleotidyltransferase: molecular cloning and structural analysis of the gene and 5' flanking region.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2833741]
2.Peterson, R C RC and 5 more authors.. (1985) Expression of human terminal deoxynucleotidyl transferase in Escherichia coli.. The Journal of biological chemistry, (5):[PMID:2863268]
3.Koiwai, O O and Morita, A A.. (1988) Isolation of putative promoter region for human terminal deoxynucleotidyltransferase gene.. Biochemical and biophysical research communications, (15):[PMID:3395350]
4.Peterson, R C RC, Cheung, L C LC, Mattaliano, R J RJ, Chang, L M LM and Bollum, F J FJ.. (1984) Molecular cloning of human terminal deoxynucleotidyltransferase.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6087320]
5.Yang, B B, Gathy, K N KN and Coleman, M S MS.. (1994) Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase.. The Journal of biological chemistry, (22):[PMID:8163485]
6.Yamashita, N N and 11 more authors.. (2001) Terminal deoxynucleotidyltransferase directly interacts with a novel nuclear protein that is homologous to p65.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:11473582]
7.Ibe, S S and 12 more authors.. (2001) Terminal deoxynucleotidyltransferase is negatively regulated by direct interaction with proliferating cell nuclear antigen.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:11554927]
8.Fujita, Kiyoko K and 9 more authors.. (2003) Terminal deoxynucleotidyltransferase forms a ternary complex with a novel chromatin remodeling protein with 82 kDa and core histone.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:12786946]
9.Mahajan, Kiran N KN and Mitchell, Beverly S BS.. (2003) Role of human Pso4 in mammalian DNA repair and association with terminal deoxynucleotidyl transferase.. Proceedings of the National Academy of Sciences of the United States of America, (16):[PMID:12960389]
10.Deloukas, P P and 135 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 10.. Nature, (27):[PMID:15164054]
11.Fujisaki, Seiichiro S and 6 more authors.. (2006) Direct binding of TReP-132 with TdT results in reduction of TdT activity.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:16371131]
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