| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab130625-10μl | 10μl | In stock | $86.90 | |
| Ab130625-50μl | 50μl | In stock | $259.90 | |
| Ab130625-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $409.90 | |
| Ab130625-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,809.90 |
Recombinant; Rabbit anti Human TdT Antibody; WB; Unconjugated
| Product Name | Recombinant TdT Antibody - Primary antibody, specific to TdT, Rabbit IgG |
|---|---|
| Synonyms | Deoxynucleotidyltransferase terminal antibody | DNA nucleotidylexotransferase antibody | DNTT antibody | Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody | TDT antibody | TDT_HUMAN antibody | Terminal addition enzyme antibody | Terminal |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | TdT |
| Immunogen | A synthetic peptide derived from human TdT (AA 495-509). |
| Positive Control | WB: MOLT-4, Jurkat and RAW 264.7 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human TdT Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant TdT Antibody (Ab130625) - Western Blot
All lanes: Recombinant TdT Antibody (Ab130625) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 56 kDa
Exposure time: 15.2 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102618 | Certificate of Analysis | Jan 30, 2024 | Ab130625 |
| ZJ24F0102617 | Certificate of Analysis | Jan 30, 2024 | Ab130625 |
| 1. Riley, L K LK, Morrow, J K JK, Danton, M J MJ and Coleman, M S MS.. (1988) Human terminal deoxyribonucleotidyltransferase: molecular cloning and structural analysis of the gene and 5' flanking region.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2833741] |
| 2. Peterson, R C RC and 5 more authors.. (1985) Expression of human terminal deoxynucleotidyl transferase in Escherichia coli.. The Journal of biological chemistry, (5): [PMID:2863268] |
| 3. Koiwai, O O and Morita, A A.. (1988) Isolation of putative promoter region for human terminal deoxynucleotidyltransferase gene.. Biochemical and biophysical research communications, (15): [PMID:3395350] |
| 4. Peterson, R C RC, Cheung, L C LC, Mattaliano, R J RJ, Chang, L M LM and Bollum, F J FJ.. (1984) Molecular cloning of human terminal deoxynucleotidyltransferase.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6087320] |
| 5. Yang, B B, Gathy, K N KN and Coleman, M S MS.. (1994) Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase.. The Journal of biological chemistry, (22): [PMID:8163485] |
| 6. Yamashita, N N and 11 more authors.. (2001) Terminal deoxynucleotidyltransferase directly interacts with a novel nuclear protein that is homologous to p65.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:11473582] |
| 7. Ibe, S S and 12 more authors.. (2001) Terminal deoxynucleotidyltransferase is negatively regulated by direct interaction with proliferating cell nuclear antigen.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:11554927] |
| 8. Fujita, Kiyoko K and 9 more authors.. (2003) Terminal deoxynucleotidyltransferase forms a ternary complex with a novel chromatin remodeling protein with 82 kDa and core histone.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:12786946] |
| 9. Mahajan, Kiran N KN and Mitchell, Beverly S BS.. (2003) Role of human Pso4 in mammalian DNA repair and association with terminal deoxynucleotidyl transferase.. Proceedings of the National Academy of Sciences of the United States of America, (16): [PMID:12960389] |
| 10. Deloukas, P P and 135 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 10.. Nature, (27): [PMID:15164054] |
| 11. Fujisaki, Seiichiro S and 6 more authors.. (2006) Direct binding of TReP-132 with TdT results in reduction of TdT activity.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:16371131] |
