Recombinant TdT Antibody - Primary antibody, specific to TdT, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human TdT Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab130625
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab130625-10μl
10μl
In stock
$86.90
Ab130625-50μl
50μl
In stock
$259.90
Ab130625-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$409.90
Ab130625-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,809.90

Recombinant; Rabbit anti Human TdT Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant TdT Antibody - Primary antibody, specific to TdT, Rabbit IgG
SynonymsDeoxynucleotidyltransferase terminal antibody; DNA nucleotidylexotransferase antibody; DNTT antibody; Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody; TDT antibody; TDT_HUMAN antibody; Terminal addition enzyme antibody; Terminal deoxynu
Specifications & PurityExactAb™, Validated, Recombinant, 0.2 mg/mL
Host speciesRabbit
SpecificityTdT
ImmunogenA synthetic peptide derived from human TdT (AA 495-509).
Positive ControlWB: MOLT-4, Jurkat and RAW 264.7 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human TdT Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function:
Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.2 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant TdT Antibody (Ab130625) - Western Blot
All lanes: Recombinant TdT Antibody (Ab130625) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 59 kDa
Observed band size: 56 kDa
Exposure time: 15.2 s

Associated Targets(Human)

DNTT Tchem DNA nucleotidylexotransferase (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
DNTT Tchem Terminal deoxynucleotidyltransferase (76 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
WB

1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0102618Certificate of AnalysisJan 30, 2024 Ab130625
ZJ24F0102617Certificate of AnalysisJan 30, 2024 Ab130625

Related Documents

References

1. Riley, L K LK, Morrow, J K JK, Danton, M J MJ and Coleman, M S MS..  (1988)  Human terminal deoxyribonucleotidyltransferase: molecular cloning and structural analysis of the gene and 5' flanking region..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2833741]
2. Peterson, R C RC and 5 more authors..  (1985)  Expression of human terminal deoxynucleotidyl transferase in Escherichia coli..  The Journal of biological chemistry,    (5):   [PMID:2863268]
3. Koiwai, O O and Morita, A A..  (1988)  Isolation of putative promoter region for human terminal deoxynucleotidyltransferase gene..  Biochemical and biophysical research communications,    (15):   [PMID:3395350]
4. Peterson, R C RC, Cheung, L C LC, Mattaliano, R J RJ, Chang, L M LM and Bollum, F J FJ..  (1984)  Molecular cloning of human terminal deoxynucleotidyltransferase..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:6087320]
5. Yang, B B, Gathy, K N KN and Coleman, M S MS..  (1994)  Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase..  The Journal of biological chemistry,    (22):   [PMID:8163485]
6. Yamashita, N N and 11 more authors..  (2001)  Terminal deoxynucleotidyltransferase directly interacts with a novel nuclear protein that is homologous to p65..  Genes to cells : devoted to molecular & cellular mechanisms,      [PMID:11473582]
7. Ibe, S S and 12 more authors..  (2001)  Terminal deoxynucleotidyltransferase is negatively regulated by direct interaction with proliferating cell nuclear antigen..  Genes to cells : devoted to molecular & cellular mechanisms,      [PMID:11554927]
8. Fujita, Kiyoko K and 9 more authors..  (2003)  Terminal deoxynucleotidyltransferase forms a ternary complex with a novel chromatin remodeling protein with 82 kDa and core histone..  Genes to cells : devoted to molecular & cellular mechanisms,      [PMID:12786946]
9. Mahajan, Kiran N KN and Mitchell, Beverly S BS..  (2003)  Role of human Pso4 in mammalian DNA repair and association with terminal deoxynucleotidyl transferase..  Proceedings of the National Academy of Sciences of the United States of America,    (16):   [PMID:12960389]
10. Deloukas, P P and 135 more authors..  (2004)  The DNA sequence and comparative analysis of human chromosome 10..  Nature,    (27):   [PMID:15164054]
11. Fujisaki, Seiichiro S and 6 more authors..  (2006)  Direct binding of TReP-132 with TdT results in reduction of TdT activity..  Genes to cells : devoted to molecular & cellular mechanisms,      [PMID:16371131]

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