Click Here for 5% Off Your First Aladdin Purchase!

Recombinant Thioredoxin/TRX Antibody - Primary antibody, specific to TXN, Rabbit IgG

  • ExactAb™
  • High performance
  • Recombinant
  • Validated
  • 0.5 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human Thioredoxin/TRX Antibody; WB, IHC, IF/ICC; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • IHC
  • WB
Item Number
Ab130970
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab130970-10μl
10μl
In stock
$66.90
Ab130970-50μl
50μl
In stock
$189.90
Ab130970-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab130970-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Recombinant; Rabbit anti Human Thioredoxin/TRX Antibody; WB, IHC, IF/ICC; Unconjugated

Basic Description

Product NameRecombinant Thioredoxin/TRX Antibody - Primary antibody, specific to TXN, Rabbit IgG
SynonymsThioredoxin1; Thioredoxin-1; TRDX;TRX;TRX1;Trx80;TXN; Trx1; TXN; TXN1; ADF antibody; ATL derived factor antibody; ATL-derived factor antibody; DKFZp686B1993 antibody; MGC61975 antibody; SASP antibody; Surface associated sulphydryl protein antibody; Surfac
Specifications & PurityExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
Host speciesRabbit
SpecificityTXN
ImmunogenA synthetic peptide derived from human Thioredoxin/TRX (AA 6-20)
Positive ControlWB: HeLa, Jurkat, MCF7 and Hep G2 cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, High performance, Recombinant, Validated
Product Description

abbit anti Human Thioredoxin/TRX Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Application:
WB: 1/1000-1/10000
IHC: 1/50-1/200
IF/ICC: 1/100-1/500
Protein Function:
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAffinity Purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Thioredoxin/TRX Antibody (Ab130970) - Western Blot
All lanes: Recombinant Thioredoxin/TRX Antibody (Ab130970) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 12 kDa
Observed band size: 10,13 kDa
Exposure time: 5.1 s


Recombinant Thioredoxin/TRX Antibody (Ab130970) - IF/ICC
Immunocytochemistry analysis of Thioredoxin (green) in HeLa using Recombinant Thioredoxin/TRX Antibody (Ab130970), and cells were counterstained with DAPI (blue).

Recombinant Thioredoxin/TRX Antibody (Ab130970) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant Thioredoxin/TRX Antibody (Ab130970). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets

TXN Tchem Thioredoxin 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/1000 - 1/10000

IF/ICC

1/100-1/500

IHC

1/50-1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303535Certificate of AnalysisMar 22, 2024 Ab130970
ZJ24F0303534Certificate of AnalysisMar 22, 2024 Ab130970

Related Documents

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
2. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al..  (2017)  Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors..  Bioorg Med Chem,  25  (4): (1320-1328).  [PMID:28038940]
3. DiRaimondo TR, Plugis NM, Jin X, Khosla C..  (2013)  Selective inhibition of extracellular thioredoxin by asymmetric disulfides..  J Med Chem,  56  (3): (1301-1310).  [PMID:23327656]
4. Zhang X, Bian J, Li X, Wu X, Dong Y, You Q..  (2017)  2-Substituted 3,7,8-trimethylnaphtho[1,2-b]furan-4,5-diones as specific L-shaped NQO1-mediated redox modulators for the treatment of non-small cell lung cancer..  Eur J Med Chem,  138  (616-629).  [PMID:28710963]
5. Chen G,Niu C,Yi J,Sun L,Cao H,Fang Y,Jin T,Li Y,Lou C,Kang J,Wei W,Zhu J.  (2019)  Novel Triapine Derivative Induces Copper-Dependent Cell Death in Hematopoietic Cancers..  J Med Chem,  62  (6.0): (3107-3121).  [PMID:30835473]
6. Tonissen, K F KF and Wells, J R JR..  (1991)  Isolation and characterization of human thioredoxin-encoding genes..  Gene,    (30):   [PMID:1874447]
7. Martin, H H and Dean, M M..  (1991)  Identification of a thioredoxin-related protein associated with plasma membranes..  Biochemical and biophysical research communications,    (28):   [PMID:1998498]
8. Forman-Kay, J D JD, Clore, G M GM, Wingfield, P T PT and Gronenborn, A M AM..  (1991)  High-resolution three-dimensional structure of reduced recombinant human thioredoxin in solution..  Biochemistry,    (12):   [PMID:2001356]
9. Jacquot, J P JP and 6 more authors..  (1990)  Human thioredoxin reactivity-structure/function relationship..  Biochemical and biophysical research communications,    (31):   [PMID:2176490]
10. Forman-Kay, J D JD and 5 more authors..  (1989)  A proton nuclear magnetic resonance assignment and secondary structure determination of recombinant human thioredoxin..  Biochemistry,    (22):   [PMID:2684271]
11. Tagaya, Y Y and 9 more authors..  (1989)  ATL-derived factor (ADF), an IL-2 receptor/Tac inducer homologous to thioredoxin; possible involvement of dithiol-reduction in the IL-2 receptor induction..  The EMBO journal,      [PMID:2785919]
12. Wollman, E E EE and 9 more authors..  (1988)  Cloning and expression of a cDNA for human thioredoxin..  The Journal of biological chemistry,    (25):   [PMID:3170595]
13. Qin, J J, Clore, G M GM, Kennedy, W M WM, Huth, J R JR and Gronenborn, A M AM..  (1995)  Solution structure of human thioredoxin in a mixed disulfide intermediate complex with its target peptide from the transcription factor NF kappa B..  Structure (London, England : 1993),    (15):   [PMID:7788295]
14. Dean, M F MF, Martin, H H and Sansom, P A PA..  (1994)  Characterization of a thioredoxin-related surface protein..  The Biochemical journal,    (15):   [PMID:7818492]
15. Qin, J J, Clore, G M GM and Gronenborn, A M AM..  (1994)  The high-resolution three-dimensional solution structures of the oxidized and reduced states of human thioredoxin..  Structure (London, England : 1993),    (15):   [PMID:7922028]
16. Qin, J J, Clore, G M GM, Kennedy, W P WP, Kuszewski, J J and Gronenborn, A M AM..  (1996)  The solution structure of human thioredoxin complexed with its target from Ref-1 reveals peptide chain reversal..  Structure (London, England : 1993),    (15):   [PMID:8736558]
17. Weichsel, A A, Gasdaska, J R JR, Powis, G G and Montfort, W R WR..  (1996)  Crystal structures of reduced, oxidized, and mutated human thioredoxins: evidence for a regulatory homodimer..  Structure (London, England : 1993),    (15):   [PMID:8805557]
18. Hirota, K K and 5 more authors..  (1997)  AP-1 transcriptional activity is regulated by a direct association between thioredoxin and Ref-1..  Proceedings of the National Academy of Sciences of the United States of America,    (15):   [PMID:9108029]
19. Andersen, J F JF and 5 more authors..  (1997)  Human thioredoxin homodimers: regulation by pH, role of aspartate 60, and crystal structure of the aspartate 60 --> asparagine mutant..  Biochemistry,    (18):   [PMID:9369469]
20. Wei, S J SJ and 9 more authors..  (2000)  Thioredoxin nuclear translocation and interaction with redox factor-1 activates the activator protein-1 transcription factor in response to ionizing radiation..  Cancer research,    (1):   [PMID:11118054]
21. Humphray, S J SJ and 147 more authors..  (2004)  DNA sequence and analysis of human chromosome 9..  Nature,    (27):   [PMID:15164053]
22. Yasinska, Inna M IM, Kozhukhar, Anna V AV and Sumbayev, Vadim V VV..  (2004)  S-nitrosation of thioredoxin in the nitrogen monoxide/superoxide system activates apoptosis signal-regulating kinase 1..  Archives of biochemistry and biophysics,    (15):   [PMID:15246877]
23. Mitchell, Douglas A DA, Morton, Sarah U SU, Fernhoff, Nathaniel B NB and Marletta, Michael A MA..  (2007)  Thioredoxin is required for S-nitrosation of procaspase-3 and the inhibition of apoptosis in Jurkat cells..  Proceedings of the National Academy of Sciences of the United States of America,    (10):   [PMID:17606900]
24. Bernal-Bayard, Joaquín J and Ramos-Morales, Francisco F..  (2009)  Salmonella type III secretion effector SlrP is an E3 ubiquitin ligase for mammalian thioredoxin..  The Journal of biological chemistry,    (2):   [PMID:19690162]
25. Balaji, Hari H and 6 more authors..  (2011)  Malassezia sympodialis thioredoxin-specific T cells are highly cross-reactive to human thioredoxin in atopic dermatitis..  The Journal of allergy and clinical immunology,      [PMID:21489611]
26. Mi, Lixin and 8 more authors..  (2011)  Identification of potential protein targets of isothiocyanates by proteomics..  Chemical research in toxicology,    (17):   [PMID:21838287]

Solution Calculators