| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab130970-10μl | 10μl | In stock | $66.90 | |
| Ab130970-50μl | 50μl | In stock | $189.90 | |
| Ab130970-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab130970-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human Thioredoxin/TRX Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | Recombinant Thioredoxin/TRX Antibody - Primary antibody, specific to TXN, Rabbit IgG |
|---|---|
| Synonyms | Thioredoxin1 | Thioredoxin-1 | TRDX | TRX | TRX1 | Trx80 | TXN | TXN1 | ADF antibody | ATL derived factor antibody | ATL-derived factor antibody | DKFZp686B1993 antibody | MGC61975 antibody | SASP antibody | Surface associated sulphydryl protein antibody |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | TXN |
| Immunogen | A synthetic peptide derived from human Thioredoxin/TRX (AA 6-20) |
| Positive Control | WB: HeLa, Jurkat, MCF7 and Hep G2 cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description | abbit anti Human Thioredoxin/TRX Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Affinity Purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Thioredoxin/TRX Antibody (Ab130970) - Western Blot
All lanes: Recombinant Thioredoxin/TRX Antibody (Ab130970) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 10,13 kDa
Exposure time: 5.1 s
Recombinant Thioredoxin/TRX Antibody (Ab130970) - IF/ICC
Immunocytochemistry analysis of Thioredoxin (green) in HeLa using Recombinant Thioredoxin/TRX Antibody (Ab130970), and cells were counterstained with DAPI (blue).
Recombinant Thioredoxin/TRX Antibody (Ab130970) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant Thioredoxin/TRX Antibody (Ab130970). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB |
1/1000 - 1/10000
|
| IF/ICC |
1/100-1/500
|
| IHC | 1/50-1/200 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303535 | Certificate of Analysis | Mar 22, 2024 | Ab130970 |
| ZJ24F0303534 | Certificate of Analysis | Mar 22, 2024 | Ab130970 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] |
| 3. DiRaimondo TR, Plugis NM, Jin X, Khosla C.. (2013) Selective inhibition of extracellular thioredoxin by asymmetric disulfides.. J Med Chem, 56 (3): (1301-1310). [PMID:23327656] |
| 4. Zhang X, Bian J, Li X, Wu X, Dong Y, You Q.. (2017) 2-Substituted 3,7,8-trimethylnaphtho[1,2-b]furan-4,5-diones as specific L-shaped NQO1-mediated redox modulators for the treatment of non-small cell lung cancer.. Eur J Med Chem, 138 (616-629). [PMID:28710963] |
| 5. Chen G,Niu C,Yi J,Sun L,Cao H,Fang Y,Jin T,Li Y,Lou C,Kang J,Wei W,Zhu J. (2019) Novel Triapine Derivative Induces Copper-Dependent Cell Death in Hematopoietic Cancers.. J Med Chem, 62 (6.0): (3107-3121). [PMID:30835473] |
| 6. Tonissen, K F KF and Wells, J R JR.. (1991) Isolation and characterization of human thioredoxin-encoding genes.. Gene, (30): [PMID:1874447] |
| 7. Martin, H H and Dean, M M.. (1991) Identification of a thioredoxin-related protein associated with plasma membranes.. Biochemical and biophysical research communications, (28): [PMID:1998498] |
| 8. Forman-Kay, J D JD, Clore, G M GM, Wingfield, P T PT and Gronenborn, A M AM.. (1991) High-resolution three-dimensional structure of reduced recombinant human thioredoxin in solution.. Biochemistry, (12): [PMID:2001356] |
| 9. Jacquot, J P JP and 6 more authors.. (1990) Human thioredoxin reactivity-structure/function relationship.. Biochemical and biophysical research communications, (31): [PMID:2176490] |
| 10. Forman-Kay, J D JD and 5 more authors.. (1989) A proton nuclear magnetic resonance assignment and secondary structure determination of recombinant human thioredoxin.. Biochemistry, (22): [PMID:2684271] |
| 11. Tagaya, Y Y and 9 more authors.. (1989) ATL-derived factor (ADF), an IL-2 receptor/Tac inducer homologous to thioredoxin; possible involvement of dithiol-reduction in the IL-2 receptor induction.. The EMBO journal, [PMID:2785919] |
| 12. Wollman, E E EE and 9 more authors.. (1988) Cloning and expression of a cDNA for human thioredoxin.. The Journal of biological chemistry, (25): [PMID:3170595] |
| 13. Qin, J J, Clore, G M GM, Kennedy, W M WM, Huth, J R JR and Gronenborn, A M AM.. (1995) Solution structure of human thioredoxin in a mixed disulfide intermediate complex with its target peptide from the transcription factor NF kappa B.. Structure (London, England : 1993), (15): [PMID:7788295] |
| 14. Dean, M F MF, Martin, H H and Sansom, P A PA.. (1994) Characterization of a thioredoxin-related surface protein.. The Biochemical journal, (15): [PMID:7818492] |
| 15. Qin, J J, Clore, G M GM and Gronenborn, A M AM.. (1994) The high-resolution three-dimensional solution structures of the oxidized and reduced states of human thioredoxin.. Structure (London, England : 1993), (15): [PMID:7922028] |
| 16. Qin, J J, Clore, G M GM, Kennedy, W P WP, Kuszewski, J J and Gronenborn, A M AM.. (1996) The solution structure of human thioredoxin complexed with its target from Ref-1 reveals peptide chain reversal.. Structure (London, England : 1993), (15): [PMID:8736558] |
| 17. Weichsel, A A, Gasdaska, J R JR, Powis, G G and Montfort, W R WR.. (1996) Crystal structures of reduced, oxidized, and mutated human thioredoxins: evidence for a regulatory homodimer.. Structure (London, England : 1993), (15): [PMID:8805557] |
| 18. Hirota, K K and 5 more authors.. (1997) AP-1 transcriptional activity is regulated by a direct association between thioredoxin and Ref-1.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:9108029] |
| 19. Andersen, J F JF and 5 more authors.. (1997) Human thioredoxin homodimers: regulation by pH, role of aspartate 60, and crystal structure of the aspartate 60 --> asparagine mutant.. Biochemistry, (18): [PMID:9369469] |
| 20. Wei, S J SJ and 9 more authors.. (2000) Thioredoxin nuclear translocation and interaction with redox factor-1 activates the activator protein-1 transcription factor in response to ionizing radiation.. Cancer research, (1): [PMID:11118054] |
| 21. Humphray, S J SJ and 147 more authors.. (2004) DNA sequence and analysis of human chromosome 9.. Nature, (27): [PMID:15164053] |
| 22. Yasinska, Inna M IM, Kozhukhar, Anna V AV and Sumbayev, Vadim V VV.. (2004) S-nitrosation of thioredoxin in the nitrogen monoxide/superoxide system activates apoptosis signal-regulating kinase 1.. Archives of biochemistry and biophysics, (15): [PMID:15246877] |
| 23. Mitchell, Douglas A DA, Morton, Sarah U SU, Fernhoff, Nathaniel B NB and Marletta, Michael A MA.. (2007) Thioredoxin is required for S-nitrosation of procaspase-3 and the inhibition of apoptosis in Jurkat cells.. Proceedings of the National Academy of Sciences of the United States of America, (10): [PMID:17606900] |
| 24. Bernal-Bayard, Joaquín J and Ramos-Morales, Francisco F.. (2009) Salmonella type III secretion effector SlrP is an E3 ubiquitin ligase for mammalian thioredoxin.. The Journal of biological chemistry, (2): [PMID:19690162] |
| 25. Balaji, Hari H and 6 more authors.. (2011) Malassezia sympodialis thioredoxin-specific T cells are highly cross-reactive to human thioredoxin in atopic dermatitis.. The Journal of allergy and clinical immunology, [PMID:21489611] |
| 26. Mi, Lixin and 8 more authors.. (2011) Identification of potential protein targets of isothiocyanates by proteomics.. Chemical research in toxicology, (17): [PMID:21838287] |
