Recombinant; Rabbit anti Human Topoisomerase I Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab131976-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab131976-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $179.90 | |
| Ab131976-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $299.90 | |
| Ab131976-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
Recombinant; Rabbit anti Human Topoisomerase I Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant Topoisomerase I Antibody - Primary antibody, specific to Topoisomerase I, Rabbit IgG |
|---|---|
| Synonyms | DNA topoisomerase 1 antibody; DNA topoisomerase I antibody; NUP98 fusion gene antibody; TOP 1 antibody; TOP I antibody; TOP1 antibody; TOP1_HUMAN antibody; TOPI antibody; Topoisomerase (DNA) I antibody; Topoisomerase 1 antibody; Topoisomerase1 antibody; T |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | Topoisomerase I |
| Immunogen | A synthetic peptide derived from human Topoisomerase I (AA 2-15). |
| Positive Control | WB: HEK293, MCF7, Hep G2, Jurkat, K562, HeLa, C6 and NIH/3T3 cel lysates. IHC: Human colon cancer tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Topoisomerase I Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Topoisomerase I Antibody (Ab131976) - Western Blot
All lanes: Recombinant Topoisomerase I Antibody (Ab131976) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 91 kDa
Observed band size: 95 kDa
Exposure time: 1.5 s
Recombinant Topoisomerase I Antibody (Ab131976) - IF/ICC
Immunocytochemistry analysis of Topoisomerase I (green) in HeLa using Recombinant Topoisomerase I Antibody (Ab131976) and cells were counterstained with DAPI (blue).
Recombinant Topoisomerase I Antibody (Ab131976) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Topoisomerase I Antibody (Ab131976). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001281 | Certificate of Analysis | Oct 13, 2023 | Ab131976 |
| ZJ23F1001280 | Certificate of Analysis | Oct 13, 2023 | Ab131976 |
