Recombinant Topoisomerase I Antibody - Primary antibody, specific to Topoisomerase I, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human Topoisomerase I Antibody; WB, IHC, ICC, IF; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • IHC
  • WB
Item Number
Ab131976
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab131976-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab131976-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$179.90
Ab131976-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$299.90
Ab131976-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90

Recombinant; Rabbit anti Human Topoisomerase I Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant Topoisomerase I Antibody - Primary antibody, specific to Topoisomerase I, Rabbit IgG
SynonymsDNA topoisomerase 1 antibody; DNA topoisomerase I antibody; NUP98 fusion gene antibody; TOP 1 antibody; TOP I antibody; TOP1 antibody; TOP1_HUMAN antibody; TOPI antibody; Topoisomerase (DNA) I antibody; Topoisomerase 1 antibody; Topoisomerase1 antibody; T
Specifications & PurityExactAb™, Validated, Recombinant, 0.2 mg/mL
Host speciesRabbit
SpecificityTopoisomerase I
ImmunogenA synthetic peptide derived from human Topoisomerase I (AA 2-15).
Positive ControlWB: HEK293, MCF7, Hep G2, Jurkat, K562, HeLa, C6 and NIH/3T3 cel lysates. IHC: Human colon cancer tissue. ICC/IF: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Topoisomerase I Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on.
Application:
WB: 1/500 – 1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.2 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Topoisomerase I Antibody (Ab131976) - Western Blot
All lanes: Recombinant Topoisomerase I Antibody (Ab131976) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 91 kDa
Observed band size: 95 kDa
Exposure time: 1.5 s

Recombinant Topoisomerase I Antibody (Ab131976) - IF/ICC
Immunocytochemistry analysis of Topoisomerase I (green) in HeLa using Recombinant Topoisomerase I Antibody (Ab131976) and cells were counterstained with DAPI (blue).

Recombinant Topoisomerase I Antibody (Ab131976) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Topoisomerase I Antibody (Ab131976). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

TOP1 Tclin DNA topoisomerase 1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
TOP1 Tclin DNA topoisomerase I (7553 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
TOP1 Tclin Topoisomerase I/II (8 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
WB

1/500-1/1000

ICC/IF

1/50-1/200

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001281Certificate of AnalysisOct 13, 2023 Ab131976
ZJ23F1001280Certificate of AnalysisOct 13, 2023 Ab131976

Related Documents

Solution Calculators