Recombinant Topoisomerase I Antibody - Primary antibody, specific to Topoisomerase I, Rabbit IgG
Synonyms
DNA topoisomerase 1 antibody; DNA topoisomerase I antibody; NUP98 fusion gene antibody; TOP 1 antibody; TOP I antibody; TOP1 antibody; TOP1_HUMAN antibody; TOPI antibody; Topoisomerase (DNA) I antibody; Topoisomerase 1 antibody; Topoisomerase1 antibody; T
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species
Rabbit
Specificity
Topoisomerase I
Immunogen
A synthetic peptide derived from human Topoisomerase I (AA 2-15).
Positive Control
WB: HEK293, MCF7, Hep G2, Jurkat, K562, HeLa, C6 and NIH/3T3 cel lysates. IHC: Human colon cancer tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Topoisomerase I Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. Application: WB: 1/500 – 1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.2 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Topoisomerase I Antibody (Ab131976) - Western Blot All lanes: Recombinant Topoisomerase I Antibody (Ab131976) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 91 kDa Observed band size: 95 kDa Exposure time: 1.5 s
Recombinant Topoisomerase I Antibody (Ab131976) - IF/ICC Immunocytochemistry analysis of Topoisomerase I (green) in HeLa using Recombinant Topoisomerase I Antibody (Ab131976) and cells were counterstained with DAPI (blue).
Recombinant Topoisomerase I Antibody (Ab131976) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Topoisomerase I Antibody (Ab131976). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.