Protein Function Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant TPX2 Antibody (Ab132114) - Western Blot All lanes: Recombinant TPX2 Antibody (Ab132114) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 50.5 s
Recombinant TPX2 Antibody (Ab132114) - IF/ICC Immunocytochemistry analysis of TPX2 (green) in HeLa using Recombinant TPX2 Antibody (Ab132114) and DAPI (blue).
Recombinant TPX2 Antibody (Ab132114) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant TPX2 Antibody (Ab132114). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Associated Targets(Human)
TPX2TbioTargeting protein for Xklp2 (0 Activities)