| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab132136-10μl | 10μl | In stock | $66.90 | |
| Ab132136-50μl | 50μl | In stock | $199.90 | |
| Ab132136-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab132136-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human TRADD Antibody; WB; Unconjugated
| Product Name | Recombinant TRADD Antibody - Primary antibody, specific to TRADD, Rabbit IgG |
|---|---|
| Synonyms | MGC11078 | TNFRSF1A-associated via death domainTNFR1-associated DEATH domain protein | TRADD | tumor necrosis factor receptor type 1 associated death domain protein | tumor necrosis factor receptor type 1-associated DEATH domain protein | tumor necrosis f |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.8 mg/mL |
| Host species | Rabbit |
| Specificity | TRADD |
| Immunogen | A synthetic peptide derived from human TRADD (AA 210-312). |
| Positive Control | WB: HeLa, MCF7 and Jurkat cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human TRADD Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant TRADD Antibody (Ab132136) - Western Blot
All lanes: Recombinant TRADD Antibody (Ab132136) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 49.1 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0302999 | Certificate of Analysis | Mar 08, 2024 | Ab132136 |
| ZJ24F0302998 | Certificate of Analysis | Mar 04, 2024 | Ab132136 |
| 1. Yuefeng Huang,Liang Chen,Yi Zhou,Heng Liu,Jueqing Yang,Zhenggang Liu,Chen Wang. (2011-02-11) UXT-V1 protects cells against TNF-induced apoptosis through modulating complex II formation.. Molecular biology of the cell, 22 ((8)): (1389-1397). [PMID:21307340] |
| 2. Hsu, H H, Xiong, J J and Goeddel, D V DV.. (1995) The TNF receptor 1-associated protein TRADD signals cell death and NF-kappa B activation.. Cell, (19): [PMID:7758105] |
| 3. Hsu, H H, Huang, J J, Shu, H B HB, Baichwal, V V and Goeddel, D V DV.. (1996) TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex.. Immunity, [PMID:8612133] |
| 4. Sanz, L L, Sanchez, P P, Lallena, M J MJ, Diaz-Meco, M T MT and Moscat, J J.. (1999) The interaction of p62 with RIP links the atypical PKCs to NF-kappaB activation.. The EMBO journal, (1): [PMID:10356400] |
| 5. Park, Y C YC and 7 more authors.. (2000) A novel mechanism of TRAF signaling revealed by structural and functional analyses of the TRADD-TRAF2 interaction.. Cell, (23): [PMID:10892748] |
| 6. Tsao, D H DH and 6 more authors.. (2000) Solution structure of N-TRADD and characterization of the interaction of N-TRADD and C-TRAF2, a key step in the TNFR1 signaling pathway.. Molecular cell, [PMID:10911999] |
| 7. Li, X X, Wang, Y Y, Debatin, K M KM and Hug, H H.. (2000) The serine/threonine kinase HIPK2 interacts with TRADD, but not with CD95 or TNF-R1 in 293T cells.. Biochemical and biophysical research communications, (22): [PMID:11032752] |
| 8. Inada, H H and 7 more authors.. (2001) Keratin attenuates tumor necrosis factor-induced cytotoxicity through association with TRADD.. The Journal of cell biology, (29): [PMID:11684708] |
| 9. Soond, Surinder M SM, Terry, Jennifer L JL, Colbert, Jeff D JD and Riches, David W H DW.. (2003) TRUSS, a novel tumor necrosis factor receptor 1 scaffolding protein that mediates activation of the transcription factor NF-kappaB.. Molecular and cellular biology, [PMID:14585990] |
| 10. Zhang, Haifeng H and 5 more authors.. (2004) AIP1/DAB2IP, a novel member of the Ras-GAP family, transduces TRAF2-induced ASK1-JNK activation.. The Journal of biological chemistry, (22): [PMID:15310755] |
| 11. Martin, Joel J and 121 more authors.. (2004) The sequence and analysis of duplication-rich human chromosome 16.. Nature, (23): [PMID:15616553] |
| 12. Liu, Xin-Yi XY, Wei, Bo B, Shi, He-Xin HX, Shan, Yu-Fei YF and Wang, Chen C.. (2010) Tom70 mediates activation of interferon regulatory factor 3 on mitochondria.. Cell research, [PMID:20628368] |
| 13. Li, Shan S and 11 more authors.. (2013) Pathogen blocks host death receptor signalling by arginine GlcNAcylation of death domains.. Nature, (12): [PMID:23955153] |
| 14. Kim, Tae Woo TW and 14 more authors.. (2014) SH3RF2 functions as an oncogene by mediating PAK4 protein stability.. Carcinogenesis, [PMID:24130170] |
| 15. Günster, Regina A; Matthews, Sophie A; Holden, David W and Thurston, Teresa L M.. (2017) SseK1 and SseK3 Type III Secretion System Effectors Inhibit NF-κB Signaling and Necroptotic Cell Death in Salmonella-Infected Macrophages.. Infection and immunity, [PMID:28069818] |
| 16. Esposito, Diego and 6 more authors.. (2018) Structural basis for the glycosyltransferase activity of the Salmonella effector SseK3.. The Journal of biological chemistry, (6): [PMID:29449376] |
| 17. Newson, Joshua P M and 11 more authors.. (2019) Salmonella Effectors SseK1 and SseK3 Target Death Domain Proteins in the TNF and TRAIL Signaling Pathways.. Molecular & cellular proteomics : MCP, [PMID:30902834] |
| 18. Xue, Juan and 6 more authors.. (2020) Arg-GlcNAcylation on TRADD by NleB and SseK1 Is Crucial for Bacterial Pathogenesis.. Frontiers in cell and developmental biology, [PMID:32766249] |
